##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062484_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1526249
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.995268137767823	32.0	32.0	32.0	32.0	32.0
2	30.551369403026637	32.0	32.0	32.0	32.0	32.0
3	30.608772225239786	32.0	32.0	32.0	32.0	32.0
4	30.445647466435688	32.0	32.0	32.0	27.0	32.0
5	30.44708432241397	32.0	32.0	32.0	27.0	32.0
6	33.78362835946166	36.0	36.0	36.0	32.0	36.0
7	33.76568240175751	36.0	36.0	36.0	32.0	36.0
8	33.72864847085895	36.0	36.0	36.0	27.0	36.0
9	33.925288730737904	36.0	36.0	36.0	32.0	36.0
10	33.5063675717396	36.0	36.0	36.0	27.0	36.0
11	33.96940800616413	36.0	36.0	36.0	32.0	36.0
12	33.67329118643157	36.0	36.0	36.0	27.0	36.0
13	33.85468622747664	36.0	36.0	36.0	32.0	36.0
14	33.67954245997868	36.0	36.0	36.0	32.0	36.0
15	33.59471488597208	36.0	36.0	36.0	27.0	36.0
16	33.61789327953696	36.0	36.0	36.0	27.0	36.0
17	33.51032957269751	36.0	36.0	36.0	27.0	36.0
18	33.56839349280491	36.0	36.0	36.0	27.0	36.0
19	33.503255366588284	36.0	36.0	36.0	27.0	36.0
20	33.48214216684171	36.0	36.0	36.0	27.0	36.0
21	33.43652772254069	36.0	36.0	36.0	27.0	36.0
22	33.39451098739459	36.0	36.0	36.0	27.0	36.0
23	33.36713799648681	36.0	36.0	36.0	27.0	36.0
24	33.34010571014297	36.0	36.0	36.0	27.0	36.0
25	32.61256190831247	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	59.0
5	148.0
6	290.0
7	68.0
8	239.0
9	258.0
10	151.0
11	66.0
12	99.0
13	81.0
14	581.0
15	1004.0
16	1382.0
17	1776.0
18	2135.0
19	2563.0
20	3386.0
21	4295.0
22	5817.0
23	7911.0
24	10449.0
25	14581.0
26	20065.0
27	26956.0
28	36930.0
29	50456.0
30	68673.0
31	98161.0
32	146790.0
33	212370.0
34	404393.0
35	404109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79466809598633	17.028845435590846	10.856451661518582	26.320034806904243
2	17.132901497708687	19.303000003931338	36.72125110896489	26.842847389395086
3	19.26342991858114	22.81647929277111	28.171934933055166	29.748155855592586
4	12.54266832239526	14.570826630144525	35.38661153396264	37.499893513497575
5	14.73651506948441	36.13995461654124	33.68279820091685	15.440732113057509
6	35.545364501701386	34.67569440210918	16.217934725251244	13.561006370938191
7	30.968218240040702	29.968010396129515	20.373948568794695	18.689822795035088
8	28.494178364039964	32.886488755110946	19.017912473810018	19.60142040703908
9	26.905702548281994	14.183245977156227	18.349125651236363	40.56192582332541
10	15.603320704022838	26.380718611265625	31.643638290949927	26.37232239376161
11	38.105930455055024	20.72750029026805	21.703916093182276	19.462653161494647
12	24.387454854994736	23.42587034321685	28.55191552594546	23.634759275842953
13	29.4162021819031	18.798398634523032	25.315376244138044	26.47002293943582
14	23.24954374713817	19.435781507687746	24.542664726635326	32.77201001853876
15	25.289318105938012	27.148772771727476	21.90336319384362	25.658545928490888
16	26.285098453100066	25.523750551808323	23.425665433283505	24.765485561808106
17	24.150704764003933	25.983412609853907	25.061548361957502	24.804334264184657
18	25.40059608040722	24.369624960063398	25.98125948391744	24.24851947561194
19	25.60987850649389	25.225368846516943	24.721917706666563	24.442834940322605
20	25.444333509575458	24.258963092800006	24.84220207318465	25.45450132443988
21	26.50168309745039	24.346754157555768	23.995242322077477	25.156320422916366
22	25.54681973813545	24.42555548118956	24.634545805298448	25.393078975376543
23	24.156363075691615	24.05912823255005	25.29778990844047	26.48671878331786
24	24.66013965093172	25.100034181215552	24.805262201414617	25.434563966438112
25	24.93559334487525	24.511070334625952	24.74685084750433	25.806485472994474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	120.0
2	326.0
3	532.0
4	532.0
5	532.0
6	1273.0
7	2014.0
8	2014.0
9	2014.0
10	2301.0
11	2588.0
12	2588.0
13	2588.0
14	3293.5
15	3999.0
16	3999.0
17	3999.0
18	6271.5
19	8544.0
20	8544.0
21	8544.0
22	14168.5
23	19793.0
24	19793.0
25	19793.0
26	29740.0
27	39687.0
28	39687.0
29	39687.0
30	49706.5
31	59726.0
32	59726.0
33	59726.0
34	75747.0
35	91768.0
36	91768.0
37	91768.0
38	105627.5
39	119487.0
40	119487.0
41	119487.0
42	137332.0
43	155177.0
44	155177.0
45	155177.0
46	179463.5
47	203750.0
48	203750.0
49	203750.0
50	204693.0
51	205636.0
52	205636.0
53	205636.0
54	192042.0
55	178448.0
56	178448.0
57	178448.0
58	165355.5
59	152263.0
60	152263.0
61	152263.0
62	135253.0
63	118243.0
64	118243.0
65	118243.0
66	98066.5
67	77890.0
68	77890.0
69	77890.0
70	58649.5
71	39409.0
72	39409.0
73	39409.0
74	30847.5
75	22286.0
76	22286.0
77	22286.0
78	18600.5
79	14915.0
80	14915.0
81	14915.0
82	10321.5
83	5728.0
84	5728.0
85	5728.0
86	4169.5
87	2611.0
88	2611.0
89	2611.0
90	1757.0
91	903.0
92	903.0
93	903.0
94	501.5
95	100.0
96	100.0
97	100.0
98	366.0
99	632.0
100	632.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007534812471621603
2	0.0033415255308930593
3	0.0043898472660751945
4	0.01533170537703874
5	0.03819822322569908
6	0.047698638950787194
7	0.06984443560650982
8	0.08740382467081059
9	0.09546279801002326
10	0.11485675011089279
11	0.11767411477419479
12	0.1306470962470737
13	0.11793619520799031
14	0.12285020334165658
15	0.11243250609828409
16	0.11243250609828409
17	0.12042595932904789
18	0.1282883723429139
19	0.1290746136443005
20	0.1199673185699057
21	0.1294677342949938
22	0.1477478445522323
23	0.1378543081764509
24	0.13235061906674467
25	0.12776421147532283
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1526249.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.85974831055217
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96386367581663	42.797294561535246
2	11.249971109891902	11.893412827397386
3	3.1974547569403664	5.070499610587366
4	1.4199646139082869	3.0023588840432973
5	0.7727867656824399	2.0424656965849715
6	0.47483116390248725	1.5059673488335212
7	0.3247210231360323	1.2015270087880958
8	0.24033107867386608	1.016307226392326
9	0.1877646905003491	0.8932674853311461
>10	0.9754177083045179	9.328106321064498
>50	0.08686526843505656	3.2235658586104163
>100	0.08914318749565264	10.175385418664657
>500	0.012415409788495445	4.443772236253342
>1k	0.00446954752385836	3.4060695159136998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2761	0.18090101942736736	No Hit
TCCATGTACTCTGCGTTGATACCAC	2369	0.155217136915405	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2041	0.13372654134417122	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2020	0.13235061906674467	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2009	0.13162989787380697	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1878	0.12304676366700323	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1792	0.11741203434039924	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1764	0.1155774713038305	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1682	0.11020482241102206	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1673	0.1096151414349821	No Hit
GAACTACGACGGTATCTGATCGTCT	1643	0.10764953818151558	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1541	0.10096648711972948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.552010844888351E-5	0.0
8	0.0	0.0	0.0	6.552010844888351E-5	0.0
9	0.0	0.0	0.0	6.552010844888351E-5	0.0
10	0.0	0.0	0.0	6.552010844888351E-5	0.0
11	0.0	0.0	0.0	1.965603253466505E-4	0.0
12	0.0	0.0	0.0	1.965603253466505E-4	1.965603253466505E-4
13	0.0	0.0	0.0	1.965603253466505E-4	1.965603253466505E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTTT	40	2.7610004E-4	16.625057	11
CGAACGA	85	1.0168151E-9	15.650194	16
ATGTACG	55	1.12579055E-5	15.549077	17
GGCGTAC	55	1.1338685E-5	15.537353	1
GTTTTCG	185	0.0	14.893897	15
TTTTCGG	205	0.0	13.905224	16
GCGTTAT	110	1.8735591E-10	13.81098	1
CGTTATT	105	1.380613E-9	13.5648	2
TTTCGGA	205	0.0	13.442158	17
GGTTCTA	400	0.0	13.063831	13
ACGAACG	105	1.9894287E-8	12.668372	15
ACGATCA	115	5.316906E-9	12.39216	9
GCGGTAA	85	3.9314655E-6	12.297387	19
CGGTTCT	395	0.0	12.266268	12
TTTACGA	55	0.0030668136	12.091743	10
CGCAAGA	325	0.0	11.978763	2
AGGCCCG	370	0.0	11.8116255	10
CGCGGTA	145	9.640644E-11	11.795854	18
GAACCGC	145	9.640644E-11	11.793531	6
GTATTAG	350	0.0	11.665346	1
>>END_MODULE