FastQCFastQC Report
Thu 2 Feb 2017
SRR4062484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526249
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC23270.15246529236055192No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18030.11813275553333696No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG17880.11714995390660371No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17340.113611868050364No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16780.10994274197722652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16740.109680661543431No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA15730.10306313059009375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA250.002353071535.19973842
TTAGGAC3200.022.6880743
TAACGCA500.002580365922.0005574
TTAACGG2200.021.99983835
CGTCTTA2900.021.2412215
TAACGGC2500.020.2398536
TTGCGAT550.00448433419.99985311
TACCGTA550.00448433419.9998537
CGCAATA6100.019.47526636
TACCGTC4000.019.2498577
CTAGCGG6400.018.90610929
GTATACG708.1161223E-418.8600921
ATTTCGT1051.7934435E-618.85700242
AGCGAAA5850.018.8032817
TATACCG951.5972093E-518.5267855
TAGCGGC6450.018.41846730
ACCGTTA600.00741328818.3331998
GGCGTTA4450.018.29242
GCGAAAG6100.018.03265418
ACCGTCG3550.017.9716978
Produced by FastQC (version 0.11.5)