##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1526249
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.087589901778806	32.0	32.0	32.0	32.0	32.0
2	30.91625285258172	32.0	32.0	32.0	32.0	32.0
3	31.219247318098162	32.0	32.0	32.0	32.0	32.0
4	31.437317239847495	32.0	32.0	32.0	32.0	32.0
5	31.253731206375893	32.0	32.0	32.0	32.0	32.0
6	34.86864659698385	36.0	36.0	36.0	32.0	36.0
7	34.89238191147054	36.0	36.0	36.0	32.0	36.0
8	34.8000876659051	36.0	36.0	36.0	32.0	36.0
9	35.02552139264301	36.0	36.0	36.0	36.0	36.0
10	34.793164156045314	36.0	36.0	36.0	32.0	36.0
11	35.03943327726996	36.0	36.0	36.0	36.0	36.0
12	34.89124513758895	36.0	36.0	36.0	32.0	36.0
13	34.97910563741565	36.0	36.0	36.0	32.0	36.0
14	34.92666399781425	36.0	36.0	36.0	32.0	36.0
15	34.85925559983987	36.0	36.0	36.0	32.0	36.0
16	34.87398648582243	36.0	36.0	36.0	32.0	36.0
17	34.834211848787454	36.0	36.0	36.0	32.0	36.0
18	34.86524217214885	36.0	36.0	36.0	32.0	36.0
19	34.85964544448514	36.0	36.0	36.0	32.0	36.0
20	34.85045428367193	36.0	36.0	36.0	32.0	36.0
21	34.850440524449155	36.0	36.0	36.0	32.0	36.0
22	34.824792022795755	36.0	36.0	36.0	32.0	36.0
23	34.76859870178457	36.0	36.0	36.0	32.0	36.0
24	34.74258852913253	36.0	36.0	36.0	32.0	36.0
25	34.71131709177205	36.0	36.0	36.0	32.0	36.0
26	34.64426053678004	36.0	36.0	36.0	32.0	36.0
27	34.6297904208291	36.0	36.0	36.0	32.0	36.0
28	34.620728989830624	36.0	36.0	36.0	32.0	36.0
29	34.58682626491483	36.0	36.0	36.0	32.0	36.0
30	34.56536187738698	36.0	36.0	36.0	32.0	36.0
31	34.569547301914696	36.0	36.0	36.0	32.0	36.0
32	34.52950534283725	36.0	36.0	36.0	32.0	36.0
33	34.52044325663768	36.0	36.0	36.0	32.0	36.0
34	34.49640065284236	36.0	36.0	36.0	32.0	36.0
35	34.47216083352061	36.0	36.0	36.0	32.0	36.0
36	34.434837303742704	36.0	36.0	36.0	32.0	36.0
37	34.42689561139762	36.0	36.0	36.0	32.0	36.0
38	34.38030688308395	36.0	36.0	36.0	32.0	36.0
39	34.36011948246977	36.0	36.0	36.0	32.0	36.0
40	34.338075897183224	36.0	36.0	36.0	32.0	36.0
41	34.307920922470714	36.0	36.0	36.0	32.0	36.0
42	34.27695546401669	36.0	36.0	36.0	32.0	36.0
43	34.269146122290664	36.0	36.0	36.0	32.0	36.0
44	34.22607975500721	36.0	36.0	36.0	32.0	36.0
45	34.16339601205308	36.0	36.0	36.0	32.0	36.0
46	34.19207612912441	36.0	36.0	36.0	32.0	36.0
47	34.17098848222014	36.0	36.0	36.0	32.0	36.0
48	34.12767379372566	36.0	36.0	36.0	32.0	36.0
49	34.12826740590821	36.0	36.0	36.0	32.0	36.0
50	33.50410319679161	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	6.0
21	26.0
22	121.0
23	356.0
24	1034.0
25	2334.0
26	5039.0
27	9589.0
28	16504.0
29	26942.0
30	40862.0
31	62427.0
32	98515.0
33	167807.0
34	349927.0
35	744757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.2284996844814	17.635556685554096	11.53421984340055	26.60172378656395
2	16.71806887568397	19.66519438159768	36.154656109720946	27.4620806329974
3	18.882576365624608	22.93579724853775	28.612416240402084	29.569210145435555
4	12.615905147787254	15.06590016825575	35.75192236124142	36.56627232271558
5	15.071656066605122	35.873962898583386	33.12342874589926	15.930952288912229
6	35.91354718744001	35.02686007405112	15.69055517371742	13.369037564791453
7	31.194647793381026	29.96784928278413	20.043649496248648	18.793853427586193
8	28.603608325776676	32.99738967716911	18.6778295532574	19.721172443796814
9	26.937806347457066	13.886135224331023	18.086039696012904	41.090018732199006
10	15.584350915217634	26.371712610458715	31.441527562016418	26.60240891230723
11	38.596651005176746	20.404665293801994	21.538425250401474	19.46025845061979
12	24.637460859925213	23.210891538667674	28.334039858502774	23.817607742904336
13	29.667570625762902	18.680569160078072	25.139934571619705	26.511925642539325
14	23.444863845938638	19.151658739825546	24.443783419350314	32.959693994885505
15	25.543276359231033	27.05738054537628	21.648761113029394	25.750581982363297
16	26.48624176002736	25.447420440570312	23.169679390453325	24.896658408948998
17	24.468550020343994	25.841720453215693	24.728599330777612	24.9611301956627
18	25.718280568898	24.276641622697216	25.69803485538729	24.307042953017497
19	25.85194159013372	25.092825613644955	24.590417422058916	24.464815374162406
20	25.72162209442889	24.07103952238462	24.676248764126953	25.53108961905954
21	26.733449129205	24.181244344795637	23.894135229572633	25.191171296426727
22	25.770696655657105	24.364635128344066	24.4977719887122	25.366896227286635
23	24.350482784919105	23.845977949862704	25.22537279303705	26.57816647218115
24	24.954250584275567	24.96296475869927	24.589172539998387	25.493612117026775
25	25.304455563934848	24.236936436977192	24.706912174881033	25.751695824206926
26	24.869729644376505	24.98517607546344	25.103570911430573	25.04152336872948
27	25.76755169045156	24.54180150158985	24.388418927711008	25.302227880247585
28	24.850597772709435	24.174823374167648	25.278837201531335	25.69574165159158
29	24.797526484865838	24.69655999774611	25.189074652956368	25.31683886443169
30	24.951498739720716	24.659868737014733	25.51444751151352	24.87418501175103
31	25.429795531397563	24.497182307736157	24.344258374616462	25.72876378624982
32	25.09027032941545	24.478115956177533	24.37262858157483	26.05898513283219
33	24.598869516048826	24.087550589713736	25.125978788520094	26.187601105717352
34	25.368272149564064	24.28843524221801	25.339246741521205	25.004045866696718
35	26.164669067760244	24.18805843607432	24.97639638093129	24.670876115234147
36	24.76568371215968	24.924635495256673	24.78448798328451	25.525192809299135
37	26.14173702980313	24.425503309093077	24.54501198690384	24.88774767419995
38	24.9785585445101	24.30796023453578	24.996314493899753	25.71716672705437
39	25.555397579294077	24.24447124944881	24.495609825133382	25.704521346123734
40	25.894464140517044	24.459442725269597	24.826486372800243	24.819606761413112
41	24.573447713970655	24.69629791731231	25.5566424613546	25.17361190736243
42	26.294726483031273	24.892334081791372	24.63025364799584	24.182685787181516
43	25.260753651599444	23.784913208788343	25.034381676908552	25.91995146270366
44	25.003128585178438	24.417902976513005	24.91251427519363	25.66645416311493
45	25.01308764166266	24.807813141892314	24.86298107320627	25.316118143238754
46	24.825323276426897	24.302079347524124	25.178870013261278	25.693727362787698
47	25.211941170805023	24.06920495934805	25.456527735644706	25.26232613420222
48	25.984292209200465	25.081556154991745	24.12666609445772	24.80748554135007
49	24.785994945778832	24.79805064573343	24.903669060553028	25.51228534793471
50	24.82329215173419	25.10850137068157	24.573267254076665	25.494939223507586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	11.0
2	13.0
3	42.5
4	72.0
5	100.0
6	128.0
7	127.0
8	126.0
9	168.0
10	210.0
11	331.0
12	452.0
13	798.5
14	1145.0
15	1498.5
16	1852.0
17	2246.0
18	2640.0
19	2907.0
20	3174.0
21	3912.5
22	4651.0
23	5160.5
24	5670.0
25	7370.5
26	9071.0
27	11369.5
28	13668.0
29	15813.5
30	17959.0
31	19604.5
32	21250.0
33	24776.0
34	28302.0
35	32840.0
36	37378.0
37	45721.5
38	54065.0
39	58396.5
40	62728.0
41	69362.5
42	75997.0
43	77819.5
44	79642.0
45	89471.5
46	99301.0
47	107120.5
48	114940.0
49	117387.5
50	119835.0
51	115154.0
52	110473.0
53	109075.5
54	107678.0
55	109013.5
56	110349.0
57	108154.5
58	105960.0
59	97420.0
60	88880.0
61	78750.5
62	68621.0
63	59872.5
64	51124.0
65	44113.5
66	37103.0
67	32599.0
68	28095.0
69	26054.5
70	24014.0
71	18628.5
72	13243.0
73	11871.0
74	10499.0
75	7783.0
76	5067.0
77	4472.5
78	3878.0
79	3372.5
80	2867.0
81	2269.0
82	1671.0
83	1420.5
84	1170.0
85	936.5
86	703.0
87	520.0
88	337.0
89	240.0
90	143.0
91	102.0
92	61.0
93	39.0
94	17.0
95	11.5
96	6.0
97	6.0
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012448820605287866
2	0.0032760054224441754
3	3.9312065069330106E-4
4	6.55201084488835E-5
5	0.0
6	3.9312065069330106E-4
7	0.0
8	6.55201084488835E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.55201084488835E-5
47	0.0
48	0.0
49	0.0
50	6.55201084488835E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1526249.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.59871805008634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.06289614803129	46.44654721455939
2	10.889736037169287	12.32690199215218
3	2.984452885265718	5.067486221607631
4	1.2236650729909089	2.770314978158031
5	0.6919931724612667	1.9582963230360004
6	0.4411571558659552	1.4981357688381114
7	0.3083050716871232	1.2214770278081801
8	0.20530930883927617	0.9296194947241833
9	0.16138658320335433	0.8220846347814106
>10	0.8677353354544072	8.741820620384832
>50	0.07563643635031438	3.0172309056880353
>100	0.07482931396493699	9.020567498720466
>500	0.009760254163686564	3.7538767425823476
>1k	0.0031372245526135384	2.4256405769591862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2327	0.15246529236055192	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1803	0.11813275553333696	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1788	0.11714995390660371	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1734	0.113611868050364	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1678	0.10994274197722652	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1674	0.109680661543431	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1573	0.10306313059009375	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3104021689776702E-4
2	0.0	0.0	0.0	0.0	1.3104021689776702E-4
3	0.0	0.0	0.0	0.0	1.3104021689776702E-4
4	0.0	0.0	0.0	0.0	1.965603253466505E-4
5	0.0	0.0	0.0	0.0	1.965603253466505E-4
6	0.0	0.0	0.0	0.0	1.965603253466505E-4
7	0.0	0.0	0.0	6.552010844888351E-5	1.965603253466505E-4
8	0.0	0.0	0.0	6.552010844888351E-5	1.965603253466505E-4
9	0.0	0.0	0.0	6.552010844888351E-5	1.965603253466505E-4
10	0.0	0.0	0.0	6.552010844888351E-5	1.965603253466505E-4
11	0.0	0.0	0.0	1.965603253466505E-4	1.965603253466505E-4
12	0.0	0.0	0.0	1.965603253466505E-4	3.93120650693301E-4
13	0.0	0.0	0.0	1.965603253466505E-4	4.586407591421845E-4
14	0.0	0.0	0.0	1.965603253466505E-4	4.586407591421845E-4
15	0.0	0.0	0.0	1.965603253466505E-4	5.241608675910681E-4
16	0.0	0.0	0.0	1.965603253466505E-4	5.241608675910681E-4
17	0.0	0.0	0.0	2.6208043379553404E-4	5.241608675910681E-4
18	0.0	0.0	0.0	3.2760054224441755E-4	5.241608675910681E-4
19	0.0	0.0	0.0	4.586407591421845E-4	5.241608675910681E-4
20	0.0	0.0	0.0	7.86241301386602E-4	6.552010844888351E-4
21	0.0	0.0	0.0	8.517614098354856E-4	7.86241301386602E-4
22	0.0	0.0	0.0	0.0012448820605287865	8.517614098354856E-4
23	0.0	0.0	0.0	0.001572482602773204	8.517614098354856E-4
24	0.0	0.0	0.0	0.0020966434703642723	8.517614098354856E-4
25	0.0	0.0	0.0	0.002489764121057573	8.517614098354856E-4
26	0.0	0.0	0.0	0.003538085856239709	8.517614098354856E-4
27	0.0	0.0	0.0	0.0038001662900352434	9.17281518284369E-4
28	0.0	0.0	0.0	0.005503689109706215	9.17281518284369E-4
29	0.0	0.0	0.0	0.010417697243372477	9.17281518284369E-4
30	0.0	0.0	0.0	0.020049153185358354	9.17281518284369E-4
31	0.0	0.0	0.0	0.0440950329860986	9.17281518284369E-4
32	0.0	0.0	0.0	0.07023755625720311	9.828016267332525E-4
33	0.0	0.0	0.0	0.10149064798732055	9.828016267332525E-4
34	0.0	0.0	0.0	0.1370025467666154	0.0011138418436310196
35	0.0	0.0	0.0	0.17631461183594552	0.001179361952079903
36	0.0	0.0	0.0	0.23718279258495828	0.001179361952079903
37	0.0	0.0	0.0	0.31606900315741404	0.001179361952079903
38	0.0	0.0	0.0	0.4328913565217733	0.0012448820605287865
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATA	25	0.0023530715	35.199738	42
TTAGGAC	320	0.0	22.688074	3
TAACGCA	50	0.0025803659	22.000557	4
TTAACGG	220	0.0	21.999838	35
CGTCTTA	290	0.0	21.24122	15
TAACGGC	250	0.0	20.23985	36
TTGCGAT	55	0.004484334	19.999853	11
TACCGTA	55	0.004484334	19.999853	7
CGCAATA	610	0.0	19.475266	36
TACCGTC	400	0.0	19.249857	7
CTAGCGG	640	0.0	18.906109	29
GTATACG	70	8.1161223E-4	18.860092	1
ATTTCGT	105	1.7934435E-6	18.857002	42
AGCGAAA	585	0.0	18.80328	17
TATACCG	95	1.5972093E-5	18.526785	5
TAGCGGC	645	0.0	18.418467	30
ACCGTTA	60	0.007413288	18.333199	8
GGCGTTA	445	0.0	18.292	42
GCGAAAG	610	0.0	18.032654	18
ACCGTCG	355	0.0	17.971697	8
>>END_MODULE