FastQCFastQC Report
Thu 2 Feb 2017
SRR4062483_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062483_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379086
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37690.27329695174920204No Hit
TATCAACGCAGAGTACTTTTTTTTT24530.1778714307882177No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23670.1716354165004938No Hit
GTACATGGAAGCAGTGGTATCAACG23200.16822736218045864No Hit
TCCATGTACTCTGCGTTGATACCAC20600.14937429572920036No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19500.14139799838443723No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14990.10869517927090842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT250.006026813319.00268714
TAGCGAA400.00527756114.25046310
AAGACGG2600.012.4221285
CGCAAGA2600.012.4144652
GTCTTAA1001.4564102E-712.3405441
CGGACCA2550.012.29451759
AACCGCG853.9409606E-612.2945177
ATACTTC853.9409606E-612.2945176
AGAACCG1405.2750693E-1112.2133525
GTCCTAA4550.012.1006171
GCGCAAG2700.011.9538031
TCCAACG1209.95351E-911.87926818
CGGTTCT1850.011.81205212
GGCGTCC1459.640644E-1111.7947715
TACCGTC1701.8189894E-1211.7356757
GGTTCTA1950.011.6939613
CGTCGTA1552.5465852E-1111.6455410
CGAACGA907.4319523E-611.614861516
CCAACGA907.4319523E-611.614861519
TCCGATA907.4560285E-611.6114878
Produced by FastQC (version 0.11.5)