##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062483_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1379086
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10273688515437	32.0	32.0	32.0	32.0	32.0
2	30.82065150396712	32.0	32.0	32.0	32.0	32.0
3	30.79804450193824	32.0	32.0	32.0	32.0	32.0
4	30.862292851932366	32.0	32.0	32.0	32.0	32.0
5	30.69233100763839	32.0	32.0	32.0	32.0	32.0
6	34.30663714953237	36.0	36.0	36.0	32.0	36.0
7	34.1800141543022	36.0	36.0	36.0	32.0	36.0
8	34.171327241375806	36.0	36.0	36.0	32.0	36.0
9	34.31794898940313	36.0	36.0	36.0	32.0	36.0
10	34.02178544340237	36.0	36.0	36.0	32.0	36.0
11	34.30714473209068	36.0	36.0	36.0	32.0	36.0
12	34.11403784825602	36.0	36.0	36.0	32.0	36.0
13	34.20891590517198	36.0	36.0	36.0	32.0	36.0
14	34.13321431730871	36.0	36.0	36.0	32.0	36.0
15	34.086365897413216	36.0	36.0	36.0	32.0	36.0
16	34.08747677809796	36.0	36.0	36.0	32.0	36.0
17	34.02177456663326	36.0	36.0	36.0	32.0	36.0
18	34.037849706254725	36.0	36.0	36.0	32.0	36.0
19	34.00868981339815	36.0	36.0	36.0	32.0	36.0
20	33.988642477698995	36.0	36.0	36.0	32.0	36.0
21	33.95861026796008	36.0	36.0	36.0	32.0	36.0
22	33.92189682151802	36.0	36.0	36.0	32.0	36.0
23	33.88754290885412	36.0	36.0	36.0	32.0	36.0
24	33.86830407965856	36.0	36.0	36.0	32.0	36.0
25	33.41373199350875	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	54.0
5	139.0
6	221.0
7	63.0
8	214.0
9	232.0
10	157.0
11	50.0
12	110.0
13	83.0
14	205.0
15	263.0
16	387.0
17	598.0
18	832.0
19	1279.0
20	1890.0
21	2915.0
22	4233.0
23	6454.0
24	8899.0
25	12226.0
26	16698.0
27	21645.0
28	28922.0
29	38295.0
30	49864.0
31	67965.0
32	97597.0
33	140795.0
34	311047.0
35	564743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19678427118172	17.746045604986904	11.111538160310724	25.94563196352065
2	15.9842006376886	20.578328594539837	38.25080146159329	25.18666930617827
3	19.723906783551012	24.41337261706986	28.332150131940853	27.530570467438274
4	12.400633854655823	15.788347662751612	36.483919745587855	35.32709873700471
5	14.05054834575051	37.18037343654908	33.94390400123024	14.825174216470174
6	33.05210590497801	35.96183044960359	17.480327984208703	13.505735661209695
7	28.9970127117568	30.91288249007926	21.34427245345831	18.745832344705633
8	27.175879659566686	33.94550076892875	20.18809905863381	18.690520512870755
9	27.37895209761203	14.252410216137399	18.98785075422001	39.38078693203057
10	15.316160984778362	27.63271472514006	32.57439306194721	24.47673122813437
11	37.06627250809286	21.385546760714917	22.510307096669276	19.03787363452295
12	24.02141949537121	24.14986385913959	30.03405336721728	21.79466327827192
13	29.77560107244523	20.275327733947627	25.572141398404106	24.376929795203036
14	22.766517252344673	20.31700811251523	25.920698602707464	30.995776032432637
15	24.659584801260827	28.32809077835426	22.992227274014184	24.02009714637073
16	24.576169064878766	26.302997922404494	25.334760016667225	23.78607299604952
17	22.930150873068712	26.41879564362273	26.576268183940122	24.07478529936844
18	23.607502936960984	25.73932274914069	28.021947702490575	22.63122661140775
19	24.925032419394423	25.411940502242857	25.998756954014556	23.664270124348164
20	24.96344556910929	25.17696410187905	26.464246354358	23.39534397465366
21	25.679067562239844	24.91292702424248	24.98219333141169	24.425812082105985
22	24.600101381725437	25.80048715516778	25.979285011917437	23.620126451189346
23	23.625590438258584	25.474765004665418	26.22253849421452	24.67710606286148
24	23.88297574955364	25.831492107840752	26.231273411638966	24.05425873096664
25	24.183227678302394	25.520137139770316	26.265300980700378	24.031334201226908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	153.0
2	530.5
3	908.0
4	908.0
5	908.0
6	2275.0
7	3642.0
8	3642.0
9	3642.0
10	4038.5
11	4435.0
12	4435.0
13	4435.0
14	4690.5
15	4946.0
16	4946.0
17	4946.0
18	8309.0
19	11672.0
20	11672.0
21	11672.0
22	18667.0
23	25662.0
24	25662.0
25	25662.0
26	38197.0
27	50732.0
28	50732.0
29	50732.0
30	65138.5
31	79545.0
32	79545.0
33	79545.0
34	90818.0
35	102091.0
36	102091.0
37	102091.0
38	112603.5
39	123116.0
40	123116.0
41	123116.0
42	136746.0
43	150376.0
44	150376.0
45	150376.0
46	165188.5
47	180001.0
48	180001.0
49	180001.0
50	176392.5
51	172784.0
52	172784.0
53	172784.0
54	155535.5
55	138287.0
56	138287.0
57	138287.0
58	125541.5
59	112796.0
60	112796.0
61	112796.0
62	100029.5
63	87263.0
64	87263.0
65	87263.0
66	73558.5
67	59854.0
68	59854.0
69	59854.0
70	45779.0
71	31704.0
72	31704.0
73	31704.0
74	25344.5
75	18985.0
76	18985.0
77	18985.0
78	15194.0
79	11403.0
80	11403.0
81	11403.0
82	8207.5
83	5012.0
84	5012.0
85	5012.0
86	3619.0
87	2226.0
88	2226.0
89	2226.0
90	1482.5
91	739.0
92	739.0
93	739.0
94	462.0
95	185.0
96	185.0
97	185.0
98	377.0
99	569.0
100	569.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00826634452093633
2	0.0031180071438619487
3	0.0042781958485547675
4	0.016025106483569553
5	0.035168220111001056
6	0.04386963539619719
7	0.06620326796153393
8	0.08665159388174487
9	0.094627891226508
10	0.11884683043697057
11	0.11870180684888398
12	0.1331316538635009
13	0.12131223143444282
14	0.12385014422595834
15	0.1143510992062859
16	0.11108806847433736
17	0.12348758525574184
18	0.13139137080646168
19	0.1322615123349813
20	0.12239990834509235
21	0.13001364671963894
22	0.15314490901945202
23	0.14002027429761452
24	0.132914118481371
25	0.13088378824815855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1379086.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.223034165302046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97410899052143	43.839409937830716
2	13.745697053352993	15.45649590113114
3	4.011829458504954	6.766716741326675
4	1.6054241111248222	3.610472585982822
5	0.7387787819895639	2.076819235019974
6	0.4313314922355004	1.4550459134716351
7	0.2597602679612168	1.0223157294260055
8	0.17761133685978947	0.7988678608330332
9	0.1353892102011722	0.6850792971678388
>10	0.7541739100372237	7.981306322494232
>50	0.07953583323550222	3.1733321906006124
>100	0.0778398154055834	8.692668162002661
>500	0.006583434349834726	2.5899685082459047
>1k	0.0019363042205396252	1.8515016144667735
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3769	0.27329695174920204	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2453	0.1778714307882177	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2367	0.1716354165004938	No Hit
GTACATGGAAGCAGTGGTATCAACG	2320	0.16822736218045864	No Hit
TCCATGTACTCTGCGTTGATACCAC	2060	0.14937429572920036	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1950	0.14139799838443723	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1499	0.10869517927090842	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.251179404330115E-5	0.0
3	0.0	0.0	0.0	1.450235880866023E-4	0.0
4	0.0	0.0	0.0	1.450235880866023E-4	0.0
5	0.0	0.0	0.0	1.450235880866023E-4	0.0
6	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
7	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
8	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
9	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
10	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
11	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
12	0.0	0.0	0.0	1.450235880866023E-4	7.251179404330115E-5
13	0.0	0.0	0.0	1.450235880866023E-4	2.1753538212990344E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAT	25	0.0060268133	19.002687	14
TAGCGAA	40	0.005277561	14.250463	10
AAGACGG	260	0.0	12.422128	5
CGCAAGA	260	0.0	12.414465	2
GTCTTAA	100	1.4564102E-7	12.340544	1
CGGACCA	255	0.0	12.2945175	9
AACCGCG	85	3.9409606E-6	12.294517	7
ATACTTC	85	3.9409606E-6	12.294517	6
AGAACCG	140	5.2750693E-11	12.213352	5
GTCCTAA	455	0.0	12.100617	1
GCGCAAG	270	0.0	11.953803	1
TCCAACG	120	9.95351E-9	11.879268	18
CGGTTCT	185	0.0	11.812052	12
GGCGTCC	145	9.640644E-11	11.79477	15
TACCGTC	170	1.8189894E-12	11.735675	7
GGTTCTA	195	0.0	11.69396	13
CGTCGTA	155	2.5465852E-11	11.64554	10
CGAACGA	90	7.4319523E-6	11.6148615	16
CCAACGA	90	7.4319523E-6	11.6148615	19
TCCGATA	90	7.4560285E-6	11.611487	8
>>END_MODULE