##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1379086
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23752543351176	32.0	32.0	32.0	32.0	32.0
2	31.35176413943728	32.0	32.0	32.0	32.0	32.0
3	31.45463444629269	32.0	32.0	32.0	32.0	32.0
4	31.56540781358088	32.0	32.0	32.0	32.0	32.0
5	31.49246674971684	32.0	32.0	32.0	32.0	32.0
6	35.070577179378226	36.0	36.0	36.0	36.0	36.0
7	35.08299627434402	36.0	36.0	36.0	36.0	36.0
8	35.02111035859983	36.0	36.0	36.0	36.0	36.0
9	35.158974132142596	36.0	36.0	36.0	36.0	36.0
10	35.025986776749235	36.0	36.0	36.0	36.0	36.0
11	35.16689242005212	36.0	36.0	36.0	36.0	36.0
12	35.07387066506367	36.0	36.0	36.0	36.0	36.0
13	35.11319453609129	36.0	36.0	36.0	36.0	36.0
14	35.07441740399076	36.0	36.0	36.0	36.0	36.0
15	35.03756691025795	36.0	36.0	36.0	36.0	36.0
16	35.060299357690525	36.0	36.0	36.0	36.0	36.0
17	35.030870446078055	36.0	36.0	36.0	36.0	36.0
18	35.04120482696511	36.0	36.0	36.0	36.0	36.0
19	35.02404998673034	36.0	36.0	36.0	36.0	36.0
20	34.99911680634855	36.0	36.0	36.0	36.0	36.0
21	34.98318959078694	36.0	36.0	36.0	36.0	36.0
22	34.975455482834285	36.0	36.0	36.0	36.0	36.0
23	34.934080253153176	36.0	36.0	36.0	32.0	36.0
24	34.920376249196934	36.0	36.0	36.0	32.0	36.0
25	34.89366943033284	36.0	36.0	36.0	32.0	36.0
26	34.85526283349987	36.0	36.0	36.0	32.0	36.0
27	34.83475142231884	36.0	36.0	36.0	32.0	36.0
28	34.80995456410985	36.0	36.0	36.0	32.0	36.0
29	34.78131023010893	36.0	36.0	36.0	32.0	36.0
30	34.774204799410626	36.0	36.0	36.0	32.0	36.0
31	34.75679906836847	36.0	36.0	36.0	32.0	36.0
32	34.72441167555903	36.0	36.0	36.0	32.0	36.0
33	34.69501974496152	36.0	36.0	36.0	32.0	36.0
34	34.675526399368856	36.0	36.0	36.0	32.0	36.0
35	34.64872749052633	36.0	36.0	36.0	32.0	36.0
36	34.61222577852288	36.0	36.0	36.0	32.0	36.0
37	34.59609770529177	36.0	36.0	36.0	32.0	36.0
38	34.55744166788728	36.0	36.0	36.0	32.0	36.0
39	34.520840614725984	36.0	36.0	36.0	32.0	36.0
40	34.512163853450765	36.0	36.0	36.0	32.0	36.0
41	34.4769622779145	36.0	36.0	36.0	32.0	36.0
42	34.44470250586258	36.0	36.0	36.0	32.0	36.0
43	34.43493516720495	36.0	36.0	36.0	32.0	36.0
44	34.39047311045142	36.0	36.0	36.0	32.0	36.0
45	34.33005483341866	36.0	36.0	36.0	32.0	36.0
46	34.325880329435584	36.0	36.0	36.0	32.0	36.0
47	34.28467187688077	36.0	36.0	36.0	32.0	36.0
48	34.23197683103157	36.0	36.0	36.0	32.0	36.0
49	34.21224854722621	36.0	36.0	36.0	32.0	36.0
50	33.6710778008043	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	2.0
21	26.0
22	133.0
23	367.0
24	967.0
25	2297.0
26	4614.0
27	8660.0
28	14996.0
29	23266.0
30	33980.0
31	49044.0
32	72653.0
33	117338.0
34	249078.0
35	801663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95728719747274	18.200080644927347	11.68357889423621	26.159053263363706
2	15.605722535765848	21.06691688922503	37.93562038492022	25.391740190088896
3	18.945325104653175	24.861737434911273	28.824092020834197	27.36884543960136
4	12.310055080038634	16.22033175644123	36.71001911413663	34.7595940493835
5	13.799004412345866	37.37565124702248	33.83903095168173	14.98631338894992
6	33.07772796192241	36.30618695520707	17.198289583120328	13.417795499750195
7	29.138232958809645	30.909044765188515	21.230888596424442	18.7218336795774
8	27.321646365781394	34.0247091189382	19.940960897289944	18.712683617990468
9	27.54302487299559	14.033715083758375	18.728926259856166	39.694333783389865
10	15.41288940646196	27.66926790642498	32.42060321111229	24.49723947600077
11	37.23502377661727	21.29809163460437	22.544279327032545	18.922605261745822
12	24.150995659444007	24.04404076323014	29.97586807494239	21.829095502383463
13	30.072888855372327	20.178654558163885	25.390222219644027	24.358234366819765
14	22.84230280055051	20.280751164176852	25.848931828761952	31.02801420651069
15	24.75023312541785	28.365526152828757	22.859488095738772	24.02475262601462
16	24.701360176232665	26.3473779010156	25.258758337043517	23.692503585708216
17	23.149317736529845	26.408070272629843	26.400819093225515	24.041792897614798
18	23.81722387146269	25.769531414284536	27.79340809782711	22.619836616425662
19	25.086035243632377	25.44707146617397	25.899472549210127	23.567420740983522
20	25.22359011693252	25.15281860594626	26.215261412268703	23.40832986485252
21	25.85531286663776	24.85457759704616	24.891341076626112	24.39876845968997
22	24.869442514825035	25.692451377216504	25.838997712978017	23.599108394980444
23	23.953691067852187	25.3856539766193	26.01418620738663	24.646468748141885
24	24.133375293491486	25.80905034203813	26.11229466472722	23.945279699743164
25	24.473093048584353	25.394717950874714	26.159934913413668	23.972254087127272
26	23.320010499707777	26.269862793183314	26.69710228368644	23.713024423422468
27	24.3058808515205	25.87336830335454	26.17777281474832	23.64297803037664
28	23.410650242261905	26.033474344602148	26.195755739671057	24.36011967346489
29	23.54370938433136	25.720585953305303	26.03637481636388	24.69932984599945
30	23.651896981043965	26.090758661896356	26.341576957492137	23.91576739956754
31	24.318425391889992	25.61479124579613	25.619069441644683	24.447713920669198
32	23.80243146547786	26.0859728834895	25.906651216820414	24.204944434212226
33	23.630578513595236	25.60579978333476	26.398063645051867	24.365558058018134
34	23.915259817009236	26.104753438146716	26.585796679829976	23.394190065014076
35	24.404206844243216	25.80919536562622	26.337661320613797	23.448936469516767
36	23.450749264367847	26.394293031761617	26.2196121199113	23.93534558395923
37	24.319150509830422	25.93681612314243	25.639300232182766	24.104733134844384
38	23.752688374764155	26.107726421702488	25.893236534922405	24.24634866861095
39	24.401306372481486	25.308501427757225	25.993955416848554	24.296236782912743
40	24.98785427449775	25.653657567403336	25.975247373985376	23.383240784113536
41	23.772049023773718	25.64778411208583	26.87163817194867	23.708528692191784
42	24.422189769165957	26.400238998873167	26.214536294328273	22.963034937632607
43	23.55161316988208	25.565483225846684	26.513574932962847	24.369328671308388
44	23.161354694341036	26.04014542965413	26.314602570108026	24.483897305896804
45	23.408547400234646	26.138398910582804	26.187344371562034	24.26570931762051
46	23.492533092594	25.336871911448533	26.25175388029019	24.918841115667274
47	23.896334238763934	25.36904877578338	26.60892794212979	24.125689043322897
48	23.72636659352644	26.05464778846279	25.740961767431475	24.478023850579298
49	23.481494265042212	25.80854275947983	26.42221007246829	24.287752903009675
50	23.212620532729648	26.650114641146384	25.541191774842176	24.596073051281792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	13.0
2	19.0
3	63.5
4	108.0
5	133.0
6	158.0
7	160.0
8	162.0
9	239.0
10	316.0
11	471.5
12	627.0
13	1168.0
14	1709.0
15	2486.5
16	3264.0
17	4100.5
18	4937.0
19	5699.5
20	6462.0
21	7314.5
22	8167.0
23	9176.0
24	10185.0
25	11373.0
26	12561.0
27	15915.5
28	19270.0
29	22966.0
30	26662.0
31	30633.0
32	34604.0
33	39930.0
34	45256.0
35	51791.0
36	58326.0
37	62867.5
38	67409.0
39	68376.0
40	69343.0
41	72662.0
42	75981.0
43	76416.0
44	76851.0
45	82069.5
46	87288.0
47	92085.0
48	96882.0
49	97482.5
50	98083.0
51	92601.5
52	87120.0
53	83983.0
54	80846.0
55	80266.0
56	79686.0
57	77212.5
58	74739.0
59	68122.5
60	61506.0
61	54707.5
62	47909.0
63	42817.0
64	37725.0
65	32739.5
66	27754.0
67	25408.5
68	23063.0
69	20908.0
70	18753.0
71	15213.0
72	11673.0
73	10291.0
74	8909.0
75	6924.5
76	4940.0
77	4286.0
78	3632.0
79	3117.0
80	2602.0
81	1996.0
82	1390.0
83	1207.0
84	1024.0
85	843.0
86	662.0
87	502.0
88	342.0
89	229.5
90	117.0
91	73.0
92	29.0
93	19.0
94	9.0
95	9.0
96	9.0
97	6.5
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01464738239674683
2	0.003408054320035154
3	5.07582558303108E-4
4	1.450235880866023E-4
5	7.251179404330115E-5
6	5.07582558303108E-4
7	7.251179404330115E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	7.251179404330115E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1379086.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.78411472667885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61567117807027	47.785839762026825
2	13.686139448910476	16.63799740855799
3	3.892382972933865	7.0978515956095
4	1.4451541352570973	3.51369659020807
5	0.6860048193424249	2.0849097820982276
6	0.39755007614913895	1.4498837662949509
7	0.24947723813022868	1.0614977144941962
8	0.15840788195010794	0.7702946296052449
9	0.12798851442980722	0.7001701690318733
>10	0.6130198301713129	6.984115912020478
>50	0.06449114663667178	2.7614875247255735
>100	0.05904212355898589	7.1118849466989165
>500	0.0043113548858507615	1.8034953680479118
>1k	3.5927957382089683E-4	0.2368748305803392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	7.251179404330115E-5	0.0
4	0.0	0.0	0.0	7.251179404330115E-5	0.0
5	0.0	0.0	0.0	1.450235880866023E-4	0.0
6	0.0	0.0	0.0	1.450235880866023E-4	0.0
7	0.0	0.0	0.0	1.450235880866023E-4	0.0
8	0.0	0.0	0.0	1.450235880866023E-4	0.0
9	0.0	0.0	0.0	2.1753538212990344E-4	7.251179404330115E-5
10	0.0	0.0	0.0	2.1753538212990344E-4	7.251179404330115E-5
11	0.0	0.0	0.0	2.1753538212990344E-4	7.251179404330115E-5
12	0.0	0.0	0.0	2.1753538212990344E-4	1.450235880866023E-4
13	0.0	0.0	0.0	2.1753538212990344E-4	1.450235880866023E-4
14	0.0	0.0	0.0	2.1753538212990344E-4	1.450235880866023E-4
15	0.0	0.0	0.0	2.1753538212990344E-4	2.1753538212990344E-4
16	0.0	0.0	0.0	2.1753538212990344E-4	2.1753538212990344E-4
17	0.0	0.0	0.0	2.900471761732046E-4	2.1753538212990344E-4
18	0.0	0.0	0.0	3.6255897021650574E-4	2.1753538212990344E-4
19	0.0	0.0	0.0	3.6255897021650574E-4	2.1753538212990344E-4
20	0.0	0.0	0.0	5.800943523464092E-4	2.1753538212990344E-4
21	0.0	0.0	0.0	8.701415285196138E-4	3.6255897021650574E-4
22	0.0	0.0	0.0	0.001450235880866023	3.6255897021650574E-4
23	0.0	0.0	0.0	0.002392889203428938	4.350707642598069E-4
24	0.0	0.0	0.0	0.0037706132902516594	4.350707642598069E-4
25	0.0	0.0	0.0	0.00442321943664137	5.07582558303108E-4
26	0.0	0.0	0.0	0.005800943523464092	5.800943523464092E-4
27	0.0	0.0	0.0	0.007831273756676523	5.800943523464092E-4
28	0.0	0.0	0.0	0.013559705486097313	5.800943523464092E-4
29	0.0	0.0	0.0	0.02661182841389152	5.800943523464092E-4
30	0.0	0.0	0.0	0.052933609651609835	5.800943523464092E-4
31	0.0	0.0	0.0	0.10456200701044024	5.800943523464092E-4
32	0.0	0.0	0.0	0.1706927631779309	5.800943523464092E-4
33	0.0	0.0	0.0	0.23580835422881533	5.800943523464092E-4
34	0.0	0.0	0.0	0.3207921768475643	5.800943523464092E-4
35	0.0	0.0	0.0	0.4129546670766	5.800943523464092E-4
36	0.0	0.0	0.0	0.5378924882132079	5.800943523464092E-4
37	0.0	0.0	0.0	0.7156914072073823	5.800943523464092E-4
38	0.0	0.0	0.0	0.9512822260540676	6.526061463897103E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	70	3.207154E-5	22.007654	1
ATACGAA	270	0.0	20.37007	40
CGTAACA	55	0.0044824295	20.001154	2
CAATACG	265	0.0	19.094057	38
TAGGACA	405	0.0	19.013443	4
GTCCTAC	1030	0.0	18.802654	1
TAAACGC	235	0.0	18.723127	28
CGAATGC	285	0.0	18.526041	43
TATACCG	60	0.0074117016	18.333725	5
TCCTACA	1060	0.0	18.265203	2
CGCAATA	280	0.0	18.071161	36
TGTAGGA	1040	0.0	17.981808	2
CCATTAG	125	4.836038E-7	17.606123	1
TTAGGAC	625	0.0	17.601015	3
AATACGA	300	0.0	17.59974	39
GATTTCG	415	0.0	17.493715	41
GTAGGAC	1090	0.0	17.156954	3
CCTACAG	1180	0.0	16.967081	3
GTCCTAA	665	0.0	16.878048	1
TACGAAT	300	0.0	16.866417	41
>>END_MODULE