FastQCFastQC Report
Thu 2 Feb 2017
SRR4062482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932630
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39780.20583350149795876No Hit
TATCAACGCAGAGTACTTTTTTTTT25660.13277243962889948No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23500.12159595990955331No Hit
GCGCAAGACGGACCAGAGCGAAAGC22550.11668037855150754No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20520.10617655733378868No Hit
GAATAACGCCGCCGCATCGCCAGTC19620.10151969078406109No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19560.10120923301407926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT350.002167984816.2884913
GCGTTAT1205.2750693E-1113.4494741
CGTTATT1205.2750693E-1113.4494742
AAGACGG3850.013.3234945
CAAGACG4100.013.20546154
CGCATCG3650.012.75559913
ATCGCCA3600.012.40585716
TGCGCAC701.09048335E-412.21510210
AAGGCGT802.8652408E-511.8757946
TCGTTAA2250.011.82454412
TCAATAC907.5006537E-611.6058723
GCATCGC3850.011.5993814
TCTAGAC1406.85759E-1011.530513
GTTATAT1657.2759576E-1211.5075721
AAACCGA1251.8257197E-811.4028316
CGTAAAC752.0830236E-411.39485553
CGCCAGT3700.011.30010218
CGTCGTA3300.011.22802310
CCGCATC4200.011.08551112
CATCGCC3950.011.06517715
Produced by FastQC (version 0.11.5)