##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062482_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1932630 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.1054180055158 32.0 32.0 32.0 32.0 32.0 2 30.676885901595234 32.0 32.0 32.0 32.0 32.0 3 30.662478073919996 32.0 32.0 32.0 32.0 32.0 4 30.75249168231891 32.0 32.0 32.0 32.0 32.0 5 30.504459208436174 32.0 32.0 32.0 32.0 32.0 6 34.15902992295473 36.0 36.0 36.0 32.0 36.0 7 34.020480381656085 36.0 36.0 36.0 32.0 36.0 8 34.01071958936786 36.0 36.0 36.0 32.0 36.0 9 34.21761537386877 36.0 36.0 36.0 32.0 36.0 10 33.80814485959547 36.0 36.0 36.0 32.0 36.0 11 34.197855254239045 36.0 36.0 36.0 32.0 36.0 12 33.928555388253315 36.0 36.0 36.0 32.0 36.0 13 34.08750769676555 36.0 36.0 36.0 32.0 36.0 14 33.97382840999053 36.0 36.0 36.0 32.0 36.0 15 33.89123163771648 36.0 36.0 36.0 32.0 36.0 16 33.89575707714358 36.0 36.0 36.0 32.0 36.0 17 33.818308212125444 36.0 36.0 36.0 32.0 36.0 18 33.830914867305175 36.0 36.0 36.0 32.0 36.0 19 33.82251750205678 36.0 36.0 36.0 32.0 36.0 20 33.81146054857888 36.0 36.0 36.0 32.0 36.0 21 33.79829092997625 36.0 36.0 36.0 32.0 36.0 22 33.74268949566135 36.0 36.0 36.0 32.0 36.0 23 33.70621950399197 36.0 36.0 36.0 27.0 36.0 24 33.67649420737544 36.0 36.0 36.0 27.0 36.0 25 33.21449837785815 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 90.0 5 188.0 6 334.0 7 96.0 8 322.0 9 338.0 10 238.0 11 74.0 12 135.0 13 107.0 14 266.0 15 357.0 16 570.0 17 777.0 18 1090.0 19 1679.0 20 2612.0 21 4063.0 22 6402.0 23 9814.0 24 13713.0 25 19389.0 26 26952.0 27 34724.0 28 46376.0 29 60897.0 30 77508.0 31 104552.0 32 146878.0 33 207149.0 34 437814.0 35 727116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.457692654003665 17.52202122275614 10.919690714500836 26.100595408739352 2 16.995167567282273 20.003290959640726 36.84234492258224 26.159196550494755 3 19.28950243023017 23.585677450268637 28.305596851386237 28.819223268114957 4 12.634170298275821 15.072976325582625 35.87760739704576 36.4152459790958 5 14.651293399070875 36.48314504962667 33.55031898236216 15.315242568940304 6 34.2940045193236 35.30435547870922 16.761513792219827 13.640126209747347 7 30.324342796783498 30.479472269830012 20.67395367862931 18.52223125475718 8 28.15635901855335 32.81567215505598 19.620957250225807 19.40701157616487 9 27.364864689883994 14.19138939863149 18.838998856898982 39.60474705458553 10 15.933002778340446 26.70812505277707 31.66215007182862 25.696722097053858 11 37.461093566088095 21.283580759602792 22.13895243779115 19.116373236517962 12 24.456520331168065 23.93357156957685 28.753425487962026 22.85648261129306 13 29.421196461644065 19.465309347113756 25.300384660216807 25.813109531025376 14 23.199023515313787 19.98099667959977 25.2019879918495 31.61799181323694 15 25.13858763644426 27.391993335982225 22.35611155806124 25.113307469512275 16 25.567798014960317 25.8936822693272 24.049439506019354 24.489080209693125 17 23.76736930506201 26.011487922760374 25.470248953347348 24.750893818830267 18 24.631201646380514 24.859744711541452 26.5599061579688 23.949147484109236 19 25.352716186654867 25.17530888248942 25.52856910439363 23.943405826462083 20 25.3363188043748 24.62272767540981 25.35103202352872 24.689921496686676 21 26.450335998176232 24.3813954934277 24.648069759127907 24.52019874926816 22 25.441755808976176 24.808492158827914 25.058170085703065 24.69158194649285 23 24.241639143451994 24.49004759866645 25.661308491796923 25.60700476608463 24 24.42621285979573 25.33259343082637 25.538346431887522 24.702847277490385 25 24.53350852777602 24.950856093585333 25.56793983148588 24.94769554715276 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 658.5 3 1064.0 4 1064.0 5 1064.0 6 2727.5 7 4391.0 8 4391.0 9 4391.0 10 4723.5 11 5056.0 12 5056.0 13 5056.0 14 5765.5 15 6475.0 16 6475.0 17 6475.0 18 10423.5 19 14372.0 20 14372.0 21 14372.0 22 22553.5 23 30735.0 24 30735.0 25 30735.0 26 44586.0 27 58437.0 28 58437.0 29 58437.0 30 72805.0 31 87173.0 32 87173.0 33 87173.0 34 105896.0 35 124619.0 36 124619.0 37 124619.0 38 141129.0 39 157639.0 40 157639.0 41 157639.0 42 177966.5 43 198294.0 44 198294.0 45 198294.0 46 224038.0 47 249782.0 48 249782.0 49 249782.0 50 252280.0 51 254778.0 52 254778.0 53 254778.0 54 236231.0 55 217684.0 56 217684.0 57 217684.0 58 200032.0 59 182380.0 60 182380.0 61 182380.0 62 161922.5 63 141465.0 64 141465.0 65 141465.0 66 117230.0 67 92995.0 68 92995.0 69 92995.0 70 70363.0 71 47731.0 72 47731.0 73 47731.0 74 37432.5 75 27134.0 76 27134.0 77 27134.0 78 22281.0 79 17428.0 80 17428.0 81 17428.0 82 12160.5 83 6893.0 84 6893.0 85 6893.0 86 5200.5 87 3508.0 88 3508.0 89 3508.0 90 2379.0 91 1250.0 92 1250.0 93 1250.0 94 765.0 95 280.0 96 280.0 97 280.0 98 547.0 99 814.0 100 814.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00910676125280059 2 0.0032598065848093013 3 0.005019067281373051 4 0.015057201844119154 5 0.036478787972865995 6 0.04786223953886673 7 0.0693355686292772 8 0.09137807029798772 9 0.09919125750919731 10 0.12113027325458055 11 0.12200990360286242 12 0.13520435882709056 13 0.1218546747178715 14 0.1263045694209445 15 0.11709432224481664 16 0.11331708604337094 17 0.12568365388098082 18 0.13339335516886314 19 0.13494564401877235 20 0.12382090726108981 21 0.13246198185891764 22 0.1523312791377553 23 0.14198268680502735 24 0.13691187656199066 25 0.13370381293884498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1932630.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.31645306584612 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24348886177145 43.04225243534308 2 12.849405675240536 13.958682805664385 3 3.686345261744322 6.00687598182119 4 1.4365182906145952 3.1210631324158857 5 0.7443846914387436 2.021616807773343 6 0.4536728774096337 1.4785140931840637 7 0.27220441196178435 1.034962471664695 8 0.20836595218914275 0.9054159570081526 9 0.15626719310430254 0.7639091693983224 >10 0.7786622381676982 7.648332138839775 >50 0.07314865665989594 2.783619249753717 >100 0.08185492440361997 9.506616247729237 >500 0.011187030118480195 4.033377937282251 >1k 0.004493935175799737 3.694761572121937 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3978 0.20583350149795876 No Hit TATCAACGCAGAGTACTTTTTTTTT 2566 0.13277243962889948 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2350 0.12159595990955331 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2255 0.11668037855150754 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 2052 0.10617655733378868 No Hit GAATAACGCCGCCGCATCGCCAGTC 1962 0.10151969078406109 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1956 0.10120923301407926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1742961663639705E-5 2 0.0 0.0 0.0 0.0 5.1742961663639705E-5 3 0.0 0.0 0.0 0.0 1.0348592332727941E-4 4 0.0 0.0 0.0 0.0 1.0348592332727941E-4 5 0.0 0.0 0.0 0.0 2.0697184665455882E-4 6 0.0 0.0 0.0 0.0 2.0697184665455882E-4 7 0.0 0.0 0.0 0.0 2.0697184665455882E-4 8 0.0 0.0 0.0 1.0348592332727941E-4 2.0697184665455882E-4 9 0.0 0.0 0.0 1.552288849909191E-4 2.0697184665455882E-4 10 5.1742961663639705E-5 0.0 0.0 2.0697184665455882E-4 2.0697184665455882E-4 11 5.1742961663639705E-5 0.0 0.0 2.0697184665455882E-4 2.0697184665455882E-4 12 5.1742961663639705E-5 0.0 0.0 2.0697184665455882E-4 4.1394369330911764E-4 13 5.1742961663639705E-5 0.0 0.0 2.0697184665455882E-4 4.6568665497275733E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGGTT 35 0.0021679848 16.28849 13 GCGTTAT 120 5.2750693E-11 13.449474 1 CGTTATT 120 5.2750693E-11 13.449474 2 AAGACGG 385 0.0 13.323494 5 CAAGACG 410 0.0 13.2054615 4 CGCATCG 365 0.0 12.755599 13 ATCGCCA 360 0.0 12.405857 16 TGCGCAC 70 1.09048335E-4 12.215102 10 AAGGCGT 80 2.8652408E-5 11.875794 6 TCGTTAA 225 0.0 11.824544 12 TCAATAC 90 7.5006537E-6 11.605872 3 GCATCGC 385 0.0 11.59938 14 TCTAGAC 140 6.85759E-10 11.53051 3 GTTATAT 165 7.2759576E-12 11.507572 1 AAACCGA 125 1.8257197E-8 11.40283 16 CGTAAAC 75 2.0830236E-4 11.3948555 3 CGCCAGT 370 0.0 11.300102 18 CGTCGTA 330 0.0 11.228023 10 CCGCATC 420 0.0 11.085511 12 CATCGCC 395 0.0 11.065177 15 >>END_MODULE