FastQCFastQC Report
Thu 2 Feb 2017
SRR4062482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932630
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22370.11574900524156202No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21770.11264442754174364No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20170.10436555367556127No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19610.10146794782239746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40300.018.7819941
GGCGTTA5500.016.79992942
TACCGTC5500.016.7999297
GTCCTAC6300.016.7644371
AACGCAG46300.016.3455056
ATACCGT5550.016.2521846
CGCAATA6300.016.06342536
TAGGACG5900.016.0338334
ACCGTCG5250.015.9237458
CGTCGTA5350.015.62610510
TAACGGC2403.6379788E-1215.5832736
CTACAGT9500.015.5157254
TACGACG5700.015.4385335
GATATAC3500.015.0879951
ATACGAA6600.014.99993940
GTGTATA4550.014.9912761
AGGACGT6200.014.9031645
GTATATC3700.014.8648043
TCTAGAT5350.014.8040622
CTAGCGG6400.014.78118929
Produced by FastQC (version 0.11.5)