##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1932630
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294007647609735	32.0	32.0	32.0	32.0	32.0
2	31.414361259009745	32.0	32.0	32.0	32.0	32.0
3	31.49210557633898	32.0	32.0	32.0	32.0	32.0
4	31.590178151017007	32.0	32.0	32.0	32.0	32.0
5	31.53284022290868	32.0	32.0	32.0	32.0	32.0
6	35.11442438542297	36.0	36.0	36.0	36.0	36.0
7	35.12469588074282	36.0	36.0	36.0	36.0	36.0
8	35.06610577296223	36.0	36.0	36.0	36.0	36.0
9	35.20022145987592	36.0	36.0	36.0	36.0	36.0
10	35.07219333240196	36.0	36.0	36.0	36.0	36.0
11	35.20095983193886	36.0	36.0	36.0	36.0	36.0
12	35.110077976643225	36.0	36.0	36.0	36.0	36.0
13	35.15047370681403	36.0	36.0	36.0	36.0	36.0
14	35.117175041265014	36.0	36.0	36.0	36.0	36.0
15	35.076343635357	36.0	36.0	36.0	36.0	36.0
16	35.09331066991612	36.0	36.0	36.0	36.0	36.0
17	35.06785985936263	36.0	36.0	36.0	36.0	36.0
18	35.072157629758415	36.0	36.0	36.0	36.0	36.0
19	35.057729104898506	36.0	36.0	36.0	36.0	36.0
20	35.04884897781779	36.0	36.0	36.0	36.0	36.0
21	35.047301863264046	36.0	36.0	36.0	36.0	36.0
22	35.02892069356266	36.0	36.0	36.0	36.0	36.0
23	34.990155384113876	36.0	36.0	36.0	36.0	36.0
24	34.969387829020555	36.0	36.0	36.0	36.0	36.0
25	34.94826686949908	36.0	36.0	36.0	32.0	36.0
26	34.90149692388093	36.0	36.0	36.0	32.0	36.0
27	34.886035092076604	36.0	36.0	36.0	32.0	36.0
28	34.8752425451328	36.0	36.0	36.0	32.0	36.0
29	34.846717685226864	36.0	36.0	36.0	32.0	36.0
30	34.838512803795865	36.0	36.0	36.0	32.0	36.0
31	34.83496478891459	36.0	36.0	36.0	32.0	36.0
32	34.801090224202255	36.0	36.0	36.0	32.0	36.0
33	34.78510941049244	36.0	36.0	36.0	32.0	36.0
34	34.76833330746185	36.0	36.0	36.0	32.0	36.0
35	34.74859854188334	36.0	36.0	36.0	32.0	36.0
36	34.708312506791266	36.0	36.0	36.0	32.0	36.0
37	34.706095838313594	36.0	36.0	36.0	32.0	36.0
38	34.67300983633701	36.0	36.0	36.0	32.0	36.0
39	34.66194460398524	36.0	36.0	36.0	32.0	36.0
40	34.65665129900705	36.0	36.0	36.0	32.0	36.0
41	34.62034740224461	36.0	36.0	36.0	32.0	36.0
42	34.60371462721783	36.0	36.0	36.0	32.0	36.0
43	34.58729399833388	36.0	36.0	36.0	32.0	36.0
44	34.54828860154298	36.0	36.0	36.0	32.0	36.0
45	34.515377490776814	36.0	36.0	36.0	32.0	36.0
46	34.50737181974822	36.0	36.0	36.0	32.0	36.0
47	34.490180738165094	36.0	36.0	36.0	32.0	36.0
48	34.44286904373833	36.0	36.0	36.0	32.0	36.0
49	34.437647661476845	36.0	36.0	36.0	32.0	36.0
50	33.92253302494528	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	16.0
21	55.0
22	153.0
23	500.0
24	1265.0
25	2905.0
26	6073.0
27	11375.0
28	19197.0
29	29653.0
30	43421.0
31	62239.0
32	92105.0
33	151622.0
34	332445.0
35	1179602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94627606254234	18.13687463224316	11.522970021057557	26.393879284156945
2	16.310670267422903	20.51365108506502	36.5433982024851	26.63228044502698
3	18.6066354585518	23.915757752312018	28.73290465726008	28.744702131876103
4	12.311545178190284	15.61244421447513	36.204592199728346	35.871418407606235
5	14.323494059128784	36.68727934849368	33.55341351081868	15.43581308155885
6	34.490448727169614	35.487901928054214	16.55890287909379	13.462746465682374
7	30.21335186422226	30.57808818971463	20.67986147367136	18.528698472391753
8	28.070660188447867	32.99379601889653	19.49090100019145	19.444642792464155
9	27.34651743996523	13.998385619596093	18.759721208922556	39.89537573151612
10	15.848972643496168	26.827276819670605	31.69209833232435	25.63165220450888
11	37.75078519944324	21.06155860149123	22.083171636578133	19.104484562487386
12	24.5150908347692	23.86752766954875	28.656390514480268	22.960990981201782
13	29.517807340256542	19.411268582191106	25.376404174622145	25.694519902930203
14	23.265136109860656	19.902930203919013	25.090627797353864	31.741305888866467
15	25.12043174327212	27.38584209082959	22.326104841588922	25.16762132430936
16	25.615404914546495	25.910081081220927	23.905248288601545	24.56926571563103
17	23.861939429689077	26.04337095046646	25.25423904213429	24.840450577710165
18	24.705194475921413	24.828032266910892	26.487480790425483	23.979292466742212
19	25.361812659433003	25.13579940288622	25.531684802574727	23.970703135106046
20	25.51636888592229	24.486373491045878	25.281404097007705	24.715853526024123
21	26.556764616093098	24.29658030766365	24.64631098554819	24.500344090695066
22	25.58094410207851	24.66421405028381	25.127986215675012	24.626855631962663
23	24.247993666661493	24.37600575381734	25.69063918080543	25.685361398715738
24	24.55814097887335	25.162291799258007	25.44822340541128	24.831343816457366
25	24.706850250694647	24.692620936237148	25.569715879397503	25.0308129336707
26	24.2290557426926	25.541153764559176	25.926431857106635	24.303358635641587
27	25.06051339366563	25.080537919829453	25.104753625888037	24.75419506061688
28	24.250477328821347	24.856076952132586	25.9446971225739	24.948748596472164
29	24.28235099320615	25.030398989977385	25.73296492344629	24.954285093370174
30	24.245199546731655	25.21284467280338	26.07959102363101	24.462364756833953
31	24.75647175093008	24.98564132813834	25.02843275743417	25.22945416349741
32	24.5139524896126	25.08121057833108	25.141025442014246	25.263811490042066
33	24.147922778804013	24.726512576126833	25.690846152652085	25.43471849241707
34	24.888933732789	24.86564940004036	25.9020091792014	24.343407687969243
35	25.411434159668435	24.761542561173115	25.639206676911776	24.187816602246677
36	24.12215478389552	25.333043572748014	25.49085960582212	25.053942037534345
37	25.226763529490903	25.033193109907227	25.132022166684777	24.6080211939171
38	24.20546095217398	24.946213191350648	25.727997599126578	25.120328257348795
39	25.051251403527836	24.696242943553603	25.122708433585323	25.129797219333238
40	25.170725902009178	24.774064357895718	25.81492577472149	24.24028396537361
41	24.067048529723746	25.109772693169415	26.257483325830606	24.56569545127624
42	25.229505906459078	25.432545288027196	25.5509331843136	23.787015621200126
43	24.420918644541377	24.417193151301593	25.81073459482674	25.35115360933029
44	23.95419713033535	25.04918168506129	25.651935445481026	25.344685739122337
45	24.0792081257147	25.22593564210428	25.750454044488595	24.94440218769242
46	24.006999795097663	24.881198037076974	25.87792373907446	25.233878428750906
47	24.324016663311994	24.62676488865685	26.15100984203383	24.898208605997326
48	24.75647175093008	25.44967220833786	25.263811490042066	24.530044550689993
49	23.92723904730859	25.286940593905715	25.615715372316483	25.170104986469216
50	23.694368497196564	25.728903855268577	25.47117189650569	25.10555575102917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	41.0
2	44.0
3	174.5
4	305.0
5	341.5
6	378.0
7	337.0
8	296.0
9	413.0
10	530.0
11	771.0
12	1012.0
13	1745.5
14	2479.0
15	3385.5
16	4292.0
17	4955.0
18	5618.0
19	6112.0
20	6606.0
21	7850.0
22	9094.0
23	10023.0
24	10952.0
25	12935.5
26	14919.0
27	18226.0
28	21533.0
29	24914.0
30	28295.0
31	31256.5
32	34218.0
33	39039.5
34	43861.0
35	50883.5
36	57906.0
37	67194.0
38	76482.0
39	81757.0
40	87032.0
41	94237.0
42	101442.0
43	103351.0
44	105260.0
45	115309.5
46	125359.0
47	132700.5
48	140042.0
49	143517.0
50	146992.0
51	141570.5
52	136149.0
53	132676.5
54	129204.0
55	130836.5
56	132469.0
57	128422.0
58	124375.0
59	114043.5
60	103712.0
61	91205.0
62	78698.0
63	68793.5
64	58889.0
65	50623.0
66	42357.0
67	36897.5
68	31438.0
69	28484.5
70	25531.0
71	20105.5
72	14680.0
73	13254.0
74	11828.0
75	8575.0
76	5322.0
77	4865.5
78	4409.0
79	3887.0
80	3365.0
81	2711.0
82	2057.0
83	1809.5
84	1562.0
85	1247.0
86	932.0
87	693.0
88	454.0
89	303.5
90	153.0
91	93.0
92	33.0
93	21.5
94	10.0
95	10.0
96	10.0
97	7.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01588508923073739
2	0.0037772362014456985
3	6.726585016273161E-4
4	2.587148083181985E-4
5	5.17429616636397E-5
6	4.139436933091176E-4
7	5.17429616636397E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.034859233272794E-4
47	5.17429616636397E-5
48	0.0
49	0.0
50	1.034859233272794E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1932630.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.26181394882689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73090619278278	45.65536316382498
2	12.702211884157938	14.547033872984597
3	3.603861727186042	6.190909791582753
4	1.4063605900274883	3.2212303380446583
5	0.7071368594004825	2.0245969639674097
6	0.4060809484651153	1.3951759031503554
7	0.26746652854919223	1.0720933016725709
8	0.18384538564819125	0.8421856214669646
9	0.13748171845020885	0.7085207324934774
>10	0.7100358179927905	7.251515566282429
>50	0.06496298589098712	2.6407752720306554
>100	0.06711738613032169	8.330896438394918
>500	0.009353575781155634	3.565138079450303
>1k	0.0031783995372858947	2.5545649546539613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2237	0.11574900524156202	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2177	0.11264442754174364	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2017	0.10436555367556127	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1961	0.10146794782239746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0348592332727941E-4
7	0.0	0.0	0.0	5.1742961663639705E-5	1.0348592332727941E-4
8	0.0	0.0	0.0	1.0348592332727941E-4	1.0348592332727941E-4
9	0.0	0.0	0.0	1.0348592332727941E-4	1.552288849909191E-4
10	0.0	0.0	0.0	1.0348592332727941E-4	1.552288849909191E-4
11	0.0	0.0	0.0	1.552288849909191E-4	1.552288849909191E-4
12	0.0	0.0	0.0	1.552288849909191E-4	2.0697184665455882E-4
13	0.0	0.0	0.0	1.552288849909191E-4	3.104577699818382E-4
14	0.0	0.0	0.0	2.0697184665455882E-4	3.104577699818382E-4
15	0.0	0.0	0.0	2.0697184665455882E-4	3.104577699818382E-4
16	0.0	0.0	0.0	2.0697184665455882E-4	3.104577699818382E-4
17	0.0	0.0	0.0	3.104577699818382E-4	3.6220073164547795E-4
18	0.0	0.0	0.0	3.6220073164547795E-4	3.6220073164547795E-4
19	0.0	0.0	0.0	4.6568665497275733E-4	3.6220073164547795E-4
20	0.0	0.0	0.0	7.244014632909559E-4	4.1394369330911764E-4
21	0.0	0.0	0.0	8.278873866182353E-4	4.6568665497275733E-4
22	0.0	0.0	0.0	0.0015005458882455514	4.6568665497275733E-4
23	0.0	0.0	0.0	0.002224947351536507	6.209155399636764E-4
24	0.0	0.0	0.0	0.003311549546472941	6.209155399636764E-4
25	0.0	0.0	0.0	0.004449894703073014	6.209155399636764E-4
26	0.0	0.0	0.0	0.005795211706327647	7.244014632909559E-4
27	0.0	0.0	0.0	0.008123644981191433	7.244014632909559E-4
28	0.0	0.0	0.0	0.014798487035800955	7.244014632909559E-4
29	0.0	0.0	0.0	0.031252748844838384	7.244014632909559E-4
30	0.0	0.0	0.0	0.06317815619130408	7.244014632909559E-4
31	0.0	0.0	0.0	0.12428659391606257	7.244014632909559E-4
32	0.0	0.0	0.0	0.19719242690013092	7.761444249545956E-4
33	0.0	0.0	0.0	0.2696325732292265	7.761444249545956E-4
34	0.0	0.0	0.0	0.35216259708273184	8.278873866182353E-4
35	0.0	0.0	0.0	0.4461278154639015	8.278873866182353E-4
36	0.0	0.0	0.0	0.5780723677061828	8.278873866182353E-4
37	0.0	0.0	0.0	0.7695213258616497	8.278873866182353E-4
38	0.0	0.0	0.0	1.0171631403838293	8.278873866182353E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4030	0.0	18.781994	1
GGCGTTA	550	0.0	16.799929	42
TACCGTC	550	0.0	16.799929	7
GTCCTAC	630	0.0	16.764437	1
AACGCAG	4630	0.0	16.345505	6
ATACCGT	555	0.0	16.252184	6
CGCAATA	630	0.0	16.063425	36
TAGGACG	590	0.0	16.033833	4
ACCGTCG	525	0.0	15.923745	8
CGTCGTA	535	0.0	15.626105	10
TAACGGC	240	3.6379788E-12	15.58327	36
CTACAGT	950	0.0	15.515725	4
TACGACG	570	0.0	15.438533	5
GATATAC	350	0.0	15.087995	1
ATACGAA	660	0.0	14.999939	40
GTGTATA	455	0.0	14.991276	1
AGGACGT	620	0.0	14.903164	5
GTATATC	370	0.0	14.864804	3
TCTAGAT	535	0.0	14.804062	2
CTAGCGG	640	0.0	14.781189	29
>>END_MODULE