FastQCFastQC Report
Thu 2 Feb 2017
SRR4062481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454973
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG33220.7301532178832589No Hit
TCCATGTACTCTGCGTTGATACCAC26690.5866282174986208No Hit
GAGTACATGGAAGCAGTGGTATCAA15710.34529521532046953No Hit
CATGTACTCTGCGTTGATACCACTG12000.2637519149487991No Hit
GCTTCCATGTACTCTGCGTTGATAC10610.23320065146722993No Hit
CCCATGTACTCTGCGTTGATACCAC8770.19275869117508074No Hit
GTATCAACGCAGAGTACTTTTTTTT8390.18440654720170208No Hit
GTACATGGGAAGCAGTGGTATCAAC8140.17891171564026878No Hit
CATGGAAGCAGTGGTATCAACGCAG7990.17561481670340875No Hit
GCGTTGATACCACTGCTTCCATGTA7510.16506474010545683No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6850.15055838478327285No Hit
ACGCAGAGTACATGGAAGCAGTGGT6460.14198644754743686No Hit
GTATCAACGCAGAGTACATGGAAGC6090.13385409683651556No Hit
TATCAACGCAGAGTACTTTTTTTTT6080.13363430357405823No Hit
CAGTGGTATCAACGCAGAGTACATG5130.11275394364061164No Hit
ACTCTGCGTTGATACCACTGCTTCC5030.11055601101603832No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4680.10286324683003167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGA307.701935E-419.00341214
CCCTTAT350.00217718916.2743111
AGAACCG508.7035136E-515.1977125
TGGACCG456.757026E-414.7755535
TAAGCTG456.757026E-414.7755535
AATAGAA456.7796063E-414.7690552
TAGAACC653.3647084E-614.6131864
AACCGCG400.005269072514.2509897
CGACCAT400.005269072514.25098910
ATAGAAC801.289136E-714.2431553
GTATATA400.005296060314.2400231
CGCGCGT551.9542003E-413.8191427
CTGCGGT801.9865329E-613.06484514
TGCGGTA751.4687605E-512.67033515
GCGCGTG604.0797403E-412.6675478
GCGCGCG604.0797403E-412.6675476
TAGCGCG604.0873224E-412.6647614
GTAGGAC2200.012.5167123
AAGAATT853.91331E-612.29767716
TAAAGGT701.0891595E-412.2124464
Produced by FastQC (version 0.11.5)