##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062481_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454973
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1012235890921	32.0	32.0	32.0	32.0	32.0
2	30.690421189828847	32.0	32.0	32.0	32.0	32.0
3	30.690175021374895	32.0	32.0	32.0	32.0	32.0
4	30.760220936187423	32.0	32.0	32.0	32.0	32.0
5	30.490371956138056	32.0	32.0	32.0	32.0	32.0
6	34.14910115545318	36.0	36.0	36.0	32.0	36.0
7	34.017761493539176	36.0	36.0	36.0	32.0	36.0
8	34.00262433155374	36.0	36.0	36.0	32.0	36.0
9	34.20836181487692	36.0	36.0	36.0	32.0	36.0
10	33.794807603967705	36.0	36.0	36.0	32.0	36.0
11	34.22845091906553	36.0	36.0	36.0	32.0	36.0
12	33.946935312644925	36.0	36.0	36.0	32.0	36.0
13	34.089464649550635	36.0	36.0	36.0	32.0	36.0
14	33.95637543326747	36.0	36.0	36.0	32.0	36.0
15	33.90924076813349	36.0	36.0	36.0	32.0	36.0
16	33.89398052192108	36.0	36.0	36.0	32.0	36.0
17	33.80047827013911	36.0	36.0	36.0	32.0	36.0
18	33.835478149252815	36.0	36.0	36.0	32.0	36.0
19	33.82317192448782	36.0	36.0	36.0	32.0	36.0
20	33.800832137291664	36.0	36.0	36.0	32.0	36.0
21	33.76930279379216	36.0	36.0	36.0	32.0	36.0
22	33.71138067533678	36.0	36.0	36.0	27.0	36.0
23	33.66121725904614	36.0	36.0	36.0	27.0	36.0
24	33.61948291437074	36.0	36.0	36.0	27.0	36.0
25	33.08925804388392	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	11.0
5	35.0
6	81.0
7	22.0
8	57.0
9	80.0
10	42.0
11	15.0
12	42.0
13	19.0
14	66.0
15	105.0
16	149.0
17	205.0
18	275.0
19	442.0
20	655.0
21	980.0
22	1523.0
23	2209.0
24	3231.0
25	4387.0
26	6260.0
27	8094.0
28	10826.0
29	14280.0
30	18460.0
31	25385.0
32	35689.0
33	50148.0
34	101774.0
35	169425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.6220597577247	16.88981111670407	10.748521223817804	25.73960790175343
2	15.900588625763257	19.799629855942747	37.559071386809336	26.74071013148466
3	20.062467167371125	24.092940242966947	27.762281875949263	28.082310713712666
4	12.412537837071428	14.938306613821165	35.72061348263726	36.92854206647014
5	13.912200355736527	37.21074421048697	34.17291257631956	14.704142857456945
6	34.6790241542707	34.521147355343736	16.979451828886177	13.82037666149939
7	29.848510840448135	30.36930980940693	21.075203547063246	18.706975803081686
8	28.24200963463188	33.56745342161413	19.758914234178746	18.43162270957524
9	27.073278042985677	14.51283630683943	18.356688738808106	40.05719691136679
10	15.344570763466104	27.64355006567758	32.708314539497856	24.30356463135846
11	37.1843053728046	21.2894467010214	21.493418765429926	20.032829160744072
12	23.35157640855686	24.013300500199154	30.02515299756392	22.60997009368006
13	30.31169825602119	19.81085993161385	24.993068971435363	24.884372840929593
14	23.17749935087511	19.73163636683698	25.144457793679504	31.94640648860841
15	25.409132713455286	27.222782058446903	23.2656689358369	24.102416292260912
16	25.474367436743673	26.02574257425743	24.77931793179318	23.72057205720572
17	23.569459205520385	26.148998483918312	26.420748975159587	23.860793335401716
18	24.682354908700415	24.874900426460815	27.527847583168665	22.91489708167011
19	24.890191625516035	26.60465464275969	25.16856355682308	23.336590174901197
20	24.5467847516365	24.609714505748393	27.145387535067933	23.69811320754717
21	25.337514441326952	25.255652747978214	24.43329482312813	24.973537987566704
22	24.197272189049578	25.889553809009215	25.339765866928428	24.573408135012777
23	23.2309761019743	25.58820746100808	25.55299534099637	25.627821096021254
24	23.649411438493175	26.434102897584356	25.403102464058026	24.513383199864442
25	24.67018905126676	25.246739300261652	25.03504413250093	25.04802751597066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	67.0
2	177.5
3	288.0
4	288.0
5	288.0
6	683.0
7	1078.0
8	1078.0
9	1078.0
10	1160.0
11	1242.0
12	1242.0
13	1242.0
14	1305.0
15	1368.0
16	1368.0
17	1368.0
18	2216.5
19	3065.0
20	3065.0
21	3065.0
22	4724.0
23	6383.0
24	6383.0
25	6383.0
26	9514.0
27	12645.0
28	12645.0
29	12645.0
30	16342.0
31	20039.0
32	20039.0
33	20039.0
34	24015.5
35	27992.0
36	27992.0
37	27992.0
38	32543.0
39	37094.0
40	37094.0
41	37094.0
42	43272.0
43	49450.0
44	49450.0
45	49450.0
46	60714.0
47	71978.0
48	71978.0
49	71978.0
50	67746.5
51	63515.0
52	63515.0
53	63515.0
54	56901.5
55	50288.0
56	50288.0
57	50288.0
58	45712.0
59	41136.0
60	41136.0
61	41136.0
62	35668.5
63	30201.0
64	30201.0
65	30201.0
66	24403.0
67	18605.0
68	18605.0
69	18605.0
70	14047.0
71	9489.0
72	9489.0
73	9489.0
74	7037.5
75	4586.0
76	4586.0
77	4586.0
78	3571.0
79	2556.0
80	2556.0
81	2556.0
82	1782.5
83	1009.0
84	1009.0
85	1009.0
86	734.5
87	460.0
88	460.0
89	460.0
90	309.0
91	158.0
92	158.0
93	158.0
94	96.0
95	34.0
96	34.0
97	34.0
98	140.5
99	247.0
100	247.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007912557448463975
2	0.0032968989368599897
3	0.0030771056744026567
4	0.013627182272354623
5	0.031210643268941234
6	0.04132113334197853
7	0.06308066632525446
8	0.07978495427201174
9	0.08857668477030504
10	0.10550076597951967
11	0.10967683796620899
12	0.12176546740136228
13	0.10879766491637965
14	0.1131935301655263
15	0.104401799667233
16	0.10396221314231834
17	0.11253415037815431
18	0.11758939541467296
19	0.11978732803924629
20	0.10945704470375166
21	0.12044670782661829
22	0.13539264967371692
23	0.12835926527508226
24	0.12308422697610627
25	0.12000712130170361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	454973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.64621581615133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.12822751363578	55.883459954065664
2	9.803507838442577	12.871263826354653
3	2.2532339668141814	4.437488498092996
4	0.857797321588326	2.2524459239801526
5	0.47644934572738323	1.5638548287541951
6	0.28730998962705984	1.1316488151116502
7	0.19559772726067964	0.8988175431832297
8	0.1345375068398971	0.7065502567503056
9	0.10273165337803779	0.6069549859924249
>10	0.6503932176560833	8.355864878075645
>50	0.0661955551100616	2.964857872720136
>100	0.03864207519694017	4.473867065520959
>500	0.0036961984970986256	1.686137454335973
>1k	0.0016800902259539205	2.166788097062044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3322	0.7301532178832589	No Hit
TCCATGTACTCTGCGTTGATACCAC	2669	0.5866282174986208	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1571	0.34529521532046953	No Hit
CATGTACTCTGCGTTGATACCACTG	1200	0.2637519149487991	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1061	0.23320065146722993	No Hit
CCCATGTACTCTGCGTTGATACCAC	877	0.19275869117508074	No Hit
GTATCAACGCAGAGTACTTTTTTTT	839	0.18440654720170208	No Hit
GTACATGGGAAGCAGTGGTATCAAC	814	0.17891171564026878	No Hit
CATGGAAGCAGTGGTATCAACGCAG	799	0.17561481670340875	No Hit
GCGTTGATACCACTGCTTCCATGTA	751	0.16506474010545683	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	685	0.15055838478327285	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	646	0.14198644754743686	No Hit
GTATCAACGCAGAGTACATGGAAGC	609	0.13385409683651556	No Hit
TATCAACGCAGAGTACTTTTTTTTT	608	0.13363430357405823	No Hit
CAGTGGTATCAACGCAGAGTACATG	513	0.11275394364061164	No Hit
ACTCTGCGTTGATACCACTGCTTCC	503	0.11055601101603832	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	468	0.10286324683003167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.1979326245733264E-4	0.0
8	0.0	0.0	0.0	2.1979326245733264E-4	0.0
9	0.0	0.0	0.0	4.395865249146653E-4	0.0
10	0.0	0.0	0.0	6.593797873719979E-4	0.0
11	0.0	0.0	0.0	6.593797873719979E-4	0.0
12	0.0	0.0	0.0	8.791730498293306E-4	2.1979326245733264E-4
13	0.0	0.0	0.0	8.791730498293306E-4	2.1979326245733264E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATGA	30	7.701935E-4	19.003412	14
CCCTTAT	35	0.002177189	16.274311	1
AGAACCG	50	8.7035136E-5	15.197712	5
TGGACCG	45	6.757026E-4	14.775553	5
TAAGCTG	45	6.757026E-4	14.775553	5
AATAGAA	45	6.7796063E-4	14.769055	2
TAGAACC	65	3.3647084E-6	14.613186	4
AACCGCG	40	0.0052690725	14.250989	7
CGACCAT	40	0.0052690725	14.250989	10
ATAGAAC	80	1.289136E-7	14.243155	3
GTATATA	40	0.0052960603	14.240023	1
CGCGCGT	55	1.9542003E-4	13.819142	7
CTGCGGT	80	1.9865329E-6	13.064845	14
TGCGGTA	75	1.4687605E-5	12.670335	15
GCGCGTG	60	4.0797403E-4	12.667547	8
GCGCGCG	60	4.0797403E-4	12.667547	6
TAGCGCG	60	4.0873224E-4	12.664761	4
GTAGGAC	220	0.0	12.516712	3
AAGAATT	85	3.91331E-6	12.297677	16
TAAAGGT	70	1.0891595E-4	12.212446	4
>>END_MODULE