FastQCFastQC Report
Thu 2 Feb 2017
SRR4062481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454973
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15770.34661397489521356No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14990.3294701004235416No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA6630.14572293300921152No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC6450.14176665428497953No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5580.12264464045119161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATA250.002351255635.27
GCGAACA250.002351255635.244
TGGTATA1100.028.044
CGCGCAA407.0253457E-427.50000221
AATCGAG651.8101682E-523.69230822
TAGGACG1600.023.3750024
CGAGCCG852.3459506E-723.29411915
TTAGGAC1600.022.0000023
AGGACGT1700.022.05
GCGCAAA500.002577987722.022
TAGGACA1351.1277734E-1021.1851844
CGCAATA755.4630276E-520.53333336
GTATTAT654.925663E-420.3076931
GTAGGAC2600.020.3076933
GCGCGCA550.004479389620.020
CGCAAAT550.004479389620.023
TGTAGGA2800.019.6428572
TATGGCG3050.019.47540916
TAGGATG808.9728805E-519.2500025
CTGTAGG2750.019.21
Produced by FastQC (version 0.11.5)