##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	454973
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19560721185653	32.0	32.0	32.0	32.0	32.0
2	31.312385570132733	32.0	32.0	32.0	32.0	32.0
3	31.45381813865878	32.0	32.0	32.0	32.0	32.0
4	31.582535666951664	32.0	32.0	32.0	32.0	32.0
5	31.506421260162693	32.0	32.0	32.0	32.0	32.0
6	35.090091060348634	36.0	36.0	36.0	36.0	36.0
7	35.11318034256978	36.0	36.0	36.0	36.0	36.0
8	35.04438724935326	36.0	36.0	36.0	36.0	36.0
9	35.196470999377986	36.0	36.0	36.0	36.0	36.0
10	35.05416365366736	36.0	36.0	36.0	36.0	36.0
11	35.20844533631666	36.0	36.0	36.0	36.0	36.0
12	35.114158422587714	36.0	36.0	36.0	36.0	36.0
13	35.15485314513169	36.0	36.0	36.0	36.0	36.0
14	35.11834768217015	36.0	36.0	36.0	36.0	36.0
15	35.069593140691865	36.0	36.0	36.0	36.0	36.0
16	35.08040916713739	36.0	36.0	36.0	36.0	36.0
17	35.06454448945322	36.0	36.0	36.0	36.0	36.0
18	35.06253557903436	36.0	36.0	36.0	36.0	36.0
19	35.05402518391201	36.0	36.0	36.0	36.0	36.0
20	35.029151180399715	36.0	36.0	36.0	36.0	36.0
21	35.01553278985786	36.0	36.0	36.0	36.0	36.0
22	34.98964334147301	36.0	36.0	36.0	36.0	36.0
23	34.923667997881196	36.0	36.0	36.0	32.0	36.0
24	34.91520156141134	36.0	36.0	36.0	32.0	36.0
25	34.87949834385777	36.0	36.0	36.0	32.0	36.0
26	34.82403790994191	36.0	36.0	36.0	32.0	36.0
27	34.81803755387682	36.0	36.0	36.0	32.0	36.0
28	34.76343431368455	36.0	36.0	36.0	32.0	36.0
29	34.736023895923495	36.0	36.0	36.0	32.0	36.0
30	34.7019823154341	36.0	36.0	36.0	32.0	36.0
31	34.69494233723759	36.0	36.0	36.0	32.0	36.0
32	34.64453495042563	36.0	36.0	36.0	32.0	36.0
33	34.624683222960485	36.0	36.0	36.0	32.0	36.0
34	34.592685719811946	36.0	36.0	36.0	32.0	36.0
35	34.5500172537711	36.0	36.0	36.0	32.0	36.0
36	34.51243700175615	36.0	36.0	36.0	32.0	36.0
37	34.46223182474564	36.0	36.0	36.0	32.0	36.0
38	34.383862339083855	36.0	36.0	36.0	32.0	36.0
39	34.30039145180044	36.0	36.0	36.0	32.0	36.0
40	34.23869328509604	36.0	36.0	36.0	32.0	36.0
41	34.24040547461058	36.0	36.0	36.0	32.0	36.0
42	34.12664048196267	36.0	36.0	36.0	32.0	36.0
43	34.142327127104245	36.0	36.0	36.0	32.0	36.0
44	34.12253474381996	36.0	36.0	36.0	32.0	36.0
45	33.97661839273979	36.0	36.0	36.0	32.0	36.0
46	34.050528273106316	36.0	36.0	36.0	32.0	36.0
47	33.98744321091581	36.0	36.0	36.0	32.0	36.0
48	33.96988612511072	36.0	36.0	36.0	32.0	36.0
49	33.98627171282691	36.0	36.0	36.0	32.0	36.0
50	33.41165519712159	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	13.0
22	44.0
23	119.0
24	317.0
25	795.0
26	1703.0
27	3268.0
28	5692.0
29	8587.0
30	12012.0
31	17616.0
32	26237.0
33	39832.0
34	82300.0
35	256434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.294093078365464	17.30837292017929	11.298573114944373	26.09896088651087
2	15.520201165405354	20.282273397677542	37.334954027814106	26.862571409102998
3	19.334369305905703	24.325500530807727	28.22688238927219	28.113247774014376
4	12.269573225546242	15.564728301364264	35.78294001156117	36.38275846152832
5	13.690482731942335	37.283970697162246	33.96860912625584	15.056937444639573
6	34.984295564293674	34.71504526701937	16.720773154976076	13.579886013710885
7	29.995186527552185	30.223991313770266	20.90937264409097	18.87144951458658
8	28.181452525754274	33.8848678932596	19.580722372536393	18.352957208449734
9	27.272387592230746	14.248977411846417	18.040630982497863	40.43800401342497
10	15.237827299641957	27.7640651203478	32.854916665384536	24.143190914625702
11	37.63893681603084	21.063008134548646	21.44654737753669	19.851507671883827
12	23.56073876911377	23.882516105351307	29.748798280337514	22.807946845197407
13	30.595002340798246	19.714576469372908	24.92301740982432	24.76740378000453
14	23.05235695304996	19.49346444734083	25.321282801397004	32.1328957982122
15	25.347877786154342	27.209087132643035	23.355451861978622	24.087583219223998
16	25.617124532664576	25.9265934462045	24.708938772190876	23.747343248940048
17	23.76866319539841	26.226831042721216	26.194301639877533	23.810204122002844
18	24.841034522927735	24.717510709426712	27.562734491936887	22.87872027570867
19	25.039727632189162	26.458053554826332	25.23556342903865	23.26665538394586
20	24.751139078582685	24.42254815120897	27.195240157108223	23.63107261310012
21	25.40128754893147	25.25930110138404	24.265176175289525	25.074235174394964
22	24.260560516777918	25.888569211799386	25.36436228083864	24.486507990584055
23	23.421829427240738	25.457994210645467	25.38766036665912	25.732515995454676
24	23.841414765271786	26.359805966507903	25.388539539708948	24.410239728511364
25	24.961261437491896	24.96345937011647	25.112039615537622	24.96323957685401
26	23.5466720003165	25.558000145063552	25.823730199374467	25.071597655245476
27	25.098192640002814	25.87450244300211	25.405683414180626	23.621621502814452
28	24.250889613229795	24.907631881452303	25.673171814591196	25.168306690726705
29	23.71920971134551	25.802410252916108	26.308594136355346	24.16978589938304
30	24.87773999775811	25.304138926925333	26.102428056170368	23.71569301914619
31	24.14450967420045	25.234464462726358	25.5969035525185	25.02412231055469
32	23.859437812793285	25.77493609510894	25.77911216709563	24.586513925002144
33	23.460732834695687	25.363483107788813	26.03846821679528	25.13731584072022
34	23.518318669459507	26.02330248168573	26.595204550599703	23.86317429825506
35	24.875322271871077	25.381725948572768	26.209027788462176	23.533923991093978
36	24.061208027729116	26.097592604396304	25.854281462856036	23.98691790501854
37	24.94279880344548	25.492501752851265	25.677128093315428	23.887571350387827
38	24.1715442454827	25.950770705074806	25.206111131869363	24.67157391757313
39	24.425845050145835	25.38502284750963	25.84043448732122	24.34869761502331
40	25.088082149929775	25.52766867484444	25.23842074145059	24.14582843377519
41	23.72888061489363	25.46744532093113	26.243974917192887	24.559699146982346
42	25.374912357436596	26.029896279559445	25.433816951775164	23.1613744112288
43	24.43771388631853	24.74894114595811	25.525690535482326	25.287654432241034
44	23.901638119185094	25.470742219867994	26.469702597736568	24.157917063210345
45	24.728280579287123	25.48173188299086	25.542175030166625	24.247812507555395
46	23.928013310679972	25.213144516268	26.468164044899368	24.39067812815266
47	24.891806766555376	24.821253129306577	26.48289019348401	23.804049910654037
48	24.038129735171097	27.21348299789218	24.890707800243092	23.85767946669363
49	23.66645932835575	25.36721959325059	26.789721587874443	24.17659949051922
50	23.571948225499096	26.947972736843724	24.606734905148215	24.87334413250896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	9.5
2	10.0
3	42.0
4	74.0
5	89.0
6	104.0
7	87.0
8	70.0
9	94.5
10	119.0
11	182.5
12	246.0
13	430.5
14	615.0
15	855.0
16	1095.0
17	1217.5
18	1340.0
19	1495.5
20	1651.0
21	1862.0
22	2073.0
23	2342.0
24	2611.0
25	2972.5
26	3334.0
27	4039.0
28	4744.0
29	5612.0
30	6480.0
31	7381.0
32	8282.0
33	9692.0
34	11102.0
35	12765.5
36	14429.0
37	16084.5
38	17740.0
39	18885.5
40	20031.0
41	21798.5
42	23566.0
43	24623.5
44	25681.0
45	29537.0
46	33393.0
47	36330.5
48	39268.0
49	38526.0
50	37784.0
51	35419.5
52	33055.0
53	31952.5
54	30850.0
55	30206.5
56	29563.0
57	28366.5
58	27170.0
59	24859.5
60	22549.0
61	19913.0
62	17277.0
63	14868.5
64	12460.0
65	10559.0
66	8658.0
67	7309.5
68	5961.0
69	5226.0
70	4491.0
71	3458.5
72	2426.0
73	2158.5
74	1891.0
75	1419.0
76	947.0
77	827.0
78	707.0
79	611.0
80	515.0
81	377.5
82	240.0
83	217.0
84	194.0
85	140.5
86	87.0
87	70.5
88	54.0
89	35.5
90	17.0
91	11.0
92	5.0
93	3.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014506355322183953
2	0.005275038298975984
3	0.001318759574743996
4	4.395865249146653E-4
5	0.0
6	0.001318759574743996
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	454973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.85511499718375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.40816800094592	59.66197397404868
2	9.763676274514314	13.640854579029444
3	2.222449237651353	4.657483412146159
4	0.8358813370688546	2.3356234769977826
5	0.4459232815948653	1.5575011057865433
6	0.2601350926605054	1.0903060087561596
7	0.17458329629202335	0.8536875367346687
8	0.13210450995720385	0.7382540587765667
9	0.0876553528550557	0.5510857278427771
>10	0.5844793620778295	7.845622248688747
>50	0.054313798619172994	2.582412170164889
>100	0.029051566290622898	3.395054691118666
>500	9.473336833898764E-4	0.41161536311009217
>1k	6.315557889265843E-4	0.6785256467988443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1577	0.34661397489521356	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1499	0.3294701004235416	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	663	0.14572293300921152	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	645	0.14176665428497953	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	558	0.12264464045119161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.1979326245733264E-4	0.0
11	0.0	0.0	0.0	2.1979326245733264E-4	0.0
12	0.0	0.0	0.0	2.1979326245733264E-4	4.395865249146653E-4
13	0.0	0.0	0.0	2.1979326245733264E-4	6.593797873719979E-4
14	0.0	0.0	0.0	2.1979326245733264E-4	6.593797873719979E-4
15	0.0	0.0	0.0	4.395865249146653E-4	6.593797873719979E-4
16	0.0	0.0	0.0	4.395865249146653E-4	8.791730498293306E-4
17	0.0	0.0	0.0	4.395865249146653E-4	8.791730498293306E-4
18	0.0	0.0	0.0	4.395865249146653E-4	8.791730498293306E-4
19	0.0	0.0	0.0	6.593797873719979E-4	8.791730498293306E-4
20	0.0	0.0	0.0	8.791730498293306E-4	8.791730498293306E-4
21	0.0	0.0	0.0	8.791730498293306E-4	0.0010989663122866632
22	0.0	0.0	0.0	0.0010989663122866632	0.0010989663122866632
23	0.0	0.0	0.0	0.0026375191494879915	0.0010989663122866632
24	0.0	0.0	0.0	0.004615658511603986	0.0010989663122866632
25	0.0	0.0	0.0	0.006593797873719979	0.0010989663122866632
26	0.0	0.0	0.0	0.010769869860409299	0.0010989663122866632
27	0.0	0.0	0.0	0.014066768797269289	0.0010989663122866632
28	0.0	0.0	0.0	0.01934180709624527	0.0010989663122866632
29	0.0	0.0	0.0	0.024616845395221253	0.0010989663122866632
30	0.0	0.0	0.0	0.039782580504777204	0.0010989663122866632
31	0.0	0.0	0.0	0.06615777199965713	0.0010989663122866632
32	0.0	0.0	0.0	0.09846738158088501	0.0010989663122866632
33	0.0	0.0	0.0	0.1279196787501676	0.0010989663122866632
34	0.0	0.0	0.0	0.1615480479061395	0.0010989663122866632
35	0.0	0.0	0.0	0.20638587344743534	0.0010989663122866632
36	0.0	0.0	0.0	0.26968633303514716	0.0010989663122866632
37	0.0	0.0	0.0	0.36265888305459887	0.0010989663122866632
38	0.0	0.0	0.0	0.46354399052251455	0.0010989663122866632
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATA	25	0.0023512556	35.2	7
GCGAACA	25	0.0023512556	35.2	44
TGGTATA	110	0.0	28.0	44
CGCGCAA	40	7.0253457E-4	27.500002	21
AATCGAG	65	1.8101682E-5	23.692308	22
TAGGACG	160	0.0	23.375002	4
CGAGCCG	85	2.3459506E-7	23.294119	15
TTAGGAC	160	0.0	22.000002	3
AGGACGT	170	0.0	22.0	5
GCGCAAA	50	0.0025779877	22.0	22
TAGGACA	135	1.1277734E-10	21.185184	4
CGCAATA	75	5.4630276E-5	20.533333	36
GTATTAT	65	4.925663E-4	20.307693	1
GTAGGAC	260	0.0	20.307693	3
GCGCGCA	55	0.0044793896	20.0	20
CGCAAAT	55	0.0044793896	20.0	23
TGTAGGA	280	0.0	19.642857	2
TATGGCG	305	0.0	19.475409	16
TAGGATG	80	8.9728805E-5	19.250002	5
CTGTAGG	275	0.0	19.2	1
>>END_MODULE