FastQCFastQC Report
Thu 2 Feb 2017
SRR4062480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211528
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG75940.6268117616761643No Hit
TCCATGTACTCTGCGTTGATACCAC62170.5131536373901388No Hit
GAGTACATGGAAGCAGTGGTATCAA33350.27527221822359865No Hit
CATGTACTCTGCGTTGATACCACTG26300.21708123955863998No Hit
GTATCAACGCAGAGTACTTTTTTTT22990.18976036872445373No Hit
GCTTCCATGTACTCTGCGTTGATAC20300.16755700239697308No Hit
CCCATGTACTCTGCGTTGATACCAC17900.1477473075323063No Hit
CATGGAAGCAGTGGTATCAACGCAG17480.14428061093098962No Hit
GCGTTGATACCACTGCTTCCATGTA16840.13899802563374516No Hit
GTACATGGGAAGCAGTGGTATCAAC16380.1352011674513507No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16060.13255987480272846No Hit
TATCAACGCAGAGTACTTTTTTTTT15770.13016620333991455No Hit
GTATCAACGCAGAGTACATGGAAGC12390.1022675497388422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG456.759234E-414.7782299
CGAGCCG2000.014.72788315
CGCATCG1450.014.41602113
AGGCCCG2050.014.36629110
CAACGGA602.5635949E-514.25220114
TCCGCAC400.005298890614.2416033
CGGTCCA2100.014.02423710
GGTCGGC954.9003575E-913.999269511
AACCGCG707.2570783E-613.5718417
TAAGACT852.7044916E-713.4088484
CCGAGCC2300.013.21943414
CAAGACG1900.012.9971724
TCCAACG1650.012.67281518
TCGGCGT1001.4384932E-712.35190813
CGCAAGA1850.012.3165532
TGATCAA853.9399292E-612.29449310
GATAACG701.0910467E-412.21364811
CGTCTTA550.00306327312.09327715
ATAACGA550.003065170712.09227812
CGCGCCA550.003067069512.09127810
Produced by FastQC (version 0.11.5)