##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062480_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211528
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1096681215787	32.0	32.0	32.0	32.0	32.0
2	30.68101026142194	32.0	32.0	32.0	32.0	32.0
3	30.68210722327507	32.0	32.0	32.0	32.0	32.0
4	30.761834641873733	32.0	32.0	32.0	32.0	32.0
5	30.508850806584743	32.0	32.0	32.0	32.0	32.0
6	34.175301767685106	36.0	36.0	36.0	32.0	36.0
7	34.03748737131952	36.0	36.0	36.0	32.0	36.0
8	34.00863289994123	36.0	36.0	36.0	32.0	36.0
9	34.233936813676614	36.0	36.0	36.0	32.0	36.0
10	33.815772313970456	36.0	36.0	36.0	32.0	36.0
11	34.21408667401826	36.0	36.0	36.0	32.0	36.0
12	33.9479236138166	36.0	36.0	36.0	32.0	36.0
13	34.08550937328729	36.0	36.0	36.0	32.0	36.0
14	33.97766704525195	36.0	36.0	36.0	32.0	36.0
15	33.89585052924902	36.0	36.0	36.0	32.0	36.0
16	33.899765420196644	36.0	36.0	36.0	32.0	36.0
17	33.80662436196275	36.0	36.0	36.0	32.0	36.0
18	33.83214915379587	36.0	36.0	36.0	32.0	36.0
19	33.81817754108861	36.0	36.0	36.0	32.0	36.0
20	33.805486955315935	36.0	36.0	36.0	32.0	36.0
21	33.75656773925159	36.0	36.0	36.0	32.0	36.0
22	33.70764357076353	36.0	36.0	36.0	27.0	36.0
23	33.62994499508059	36.0	36.0	36.0	27.0	36.0
24	33.59697423419021	36.0	36.0	36.0	27.0	36.0
25	33.090569924921255	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	7.0
4	56.0
5	107.0
6	202.0
7	64.0
8	181.0
9	209.0
10	132.0
11	42.0
12	84.0
13	68.0
14	205.0
15	305.0
16	416.0
17	600.0
18	852.0
19	1311.0
20	1940.0
21	2814.0
22	4302.0
23	6223.0
24	8601.0
25	12253.0
26	16657.0
27	21149.0
28	28501.0
29	37976.0
30	48388.0
31	65364.0
32	92559.0
33	130183.0
34	271707.0
35	458069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.764310378558555	16.57818857514089	10.968944478793738	25.688556567506815
2	16.13600851188172	19.789299560951328	37.8860454500806	26.188646477086348
3	19.76265139702526	23.853972085083623	27.986647593372332	28.39672892451879
4	12.329797333553474	15.206752796466752	35.742601230032605	36.72084863994717
5	14.045117773390569	37.11131313181355	34.01610275642661	14.827466338369263
6	34.54524434755322	34.776049150274986	17.116797965284313	13.56190853688748
7	30.05900611370736	30.39153496839076	20.720033170290126	18.829425747611754
8	28.11872665178549	33.66471757437971	19.51311795836025	18.703437815474548
9	27.222164848374693	14.39409774575858	18.052933570723365	40.330803835143364
10	15.294528885175756	27.422674960065912	32.502836497898144	24.779959656860193
11	37.58253327361464	20.906473583567884	21.85770313308289	19.65329000973459
12	23.989146596161966	23.721530683676832	29.68263794244853	22.60668477771267
13	30.603173291463513	19.519791752747707	24.852656805222708	25.02437815056607
14	23.28335242326426	19.667587280651734	25.12152269883692	31.927537597247085
15	25.340401094043084	27.361571323511185	22.88797626818929	24.41005131425644
16	25.63016703919616	25.737091956254982	24.844095373059712	23.788645631489143
17	23.784841593060772	25.923765007669118	26.225075368910982	24.066318030359128
18	24.82398629411293	24.72175412874972	27.416980787460403	23.03727878967695
19	25.25259036846581	26.10600489436454	24.910844381191822	23.73056035597783
20	24.9909510563712	24.569410769151943	26.55191449202784	23.887723682449018
21	25.912038360901235	25.024421850903565	24.042423771593295	25.0211160166019
22	24.760111363876838	25.527308618135645	25.07183432970056	24.64074568828695
23	23.712441119561074	25.133175795356017	25.241121544764837	25.913261540318068
24	23.95096196601753	26.174005180516364	25.281713306191	24.593319547275104
25	24.9320649928098	24.960743152779386	24.98892543678408	25.11826641762674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	170.0
2	401.0
3	632.0
4	632.0
5	632.0
6	1643.5
7	2655.0
8	2655.0
9	2655.0
10	2756.0
11	2857.0
12	2857.0
13	2857.0
14	3153.5
15	3450.0
16	3450.0
17	3450.0
18	5710.5
19	7971.0
20	7971.0
21	7971.0
22	12888.5
23	17806.0
24	17806.0
25	17806.0
26	26999.5
27	36193.0
28	36193.0
29	36193.0
30	46470.5
31	56748.0
32	56748.0
33	56748.0
34	66846.5
35	76945.0
36	76945.0
37	76945.0
38	86973.0
39	97001.0
40	97001.0
41	97001.0
42	112681.0
43	128361.0
44	128361.0
45	128361.0
46	154633.5
47	180906.0
48	180906.0
49	180906.0
50	171084.5
51	161263.0
52	161263.0
53	161263.0
54	145946.0
55	130629.0
56	130629.0
57	130629.0
58	119917.0
59	109205.0
60	109205.0
61	109205.0
62	96063.5
63	82922.0
64	82922.0
65	82922.0
66	69027.0
67	55132.0
68	55132.0
69	55132.0
70	41830.0
71	28528.0
72	28528.0
73	28528.0
74	22185.0
75	15842.0
76	15842.0
77	15842.0
78	12499.0
79	9156.0
80	9156.0
81	9156.0
82	6532.5
83	3909.0
84	3909.0
85	3909.0
86	2883.0
87	1857.0
88	1857.0
89	1857.0
90	1243.5
91	630.0
92	630.0
93	630.0
94	387.5
95	145.0
96	145.0
97	145.0
98	380.0
99	615.0
100	615.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00965722624652505
2	0.003714317787125019
3	0.005034964111436137
4	0.014692190357961188
5	0.03557491036113074
6	0.045232136607655786
7	0.06652755858717256
8	0.08666741503291711
9	0.09434367179297548
10	0.1162994169346478
11	0.11638195732991727
12	0.13082652650207013
13	0.11786768444476727
14	0.12100421946500618
15	0.11043904887051723
16	0.11002634689417001
17	0.1221597849987784
18	0.12686458752913676
19	0.12917571859668123
20	0.1190232499785395
21	0.12760745108656177
22	0.14857271148500076
23	0.1370995965425479
24	0.13289003638380623
25	0.12777253187710064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1211528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.15612455209501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76317916445457	42.65508540949177
2	12.78486271471615	13.847572351192067
3	3.871929639635911	6.290661114632123
4	1.5774275382646321	3.4170944893665616
5	0.7843055698712992	2.123747506442597
6	0.47618220303117176	1.547290961810828
7	0.31217822034163856	1.1834453808291197
8	0.22513256514466873	0.9753845790965857
9	0.14957979736670987	0.7290595923001807
>10	0.8817242201128698	8.848859081599064
>50	0.08226476054030414	3.1336689290626585
>100	0.080536203514088	8.865901494926575
>500	0.007793822190109697	2.9239607141163657
>1k	0.0025979407300365655	2.315250522994773
>5k	3.0564008588665476E-4	1.1430178721387243
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	7594	0.6268117616761643	No Hit
TCCATGTACTCTGCGTTGATACCAC	6217	0.5131536373901388	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3335	0.27527221822359865	No Hit
CATGTACTCTGCGTTGATACCACTG	2630	0.21708123955863998	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2299	0.18976036872445373	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2030	0.16755700239697308	No Hit
CCCATGTACTCTGCGTTGATACCAC	1790	0.1477473075323063	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1748	0.14428061093098962	No Hit
GCGTTGATACCACTGCTTCCATGTA	1684	0.13899802563374516	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1638	0.1352011674513507	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1606	0.13255987480272846	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1577	0.13016620333991455	No Hit
GTATCAACGCAGAGTACATGGAAGC	1239	0.1022675497388422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.254039526944487E-5
2	0.0	0.0	0.0	0.0	8.254039526944487E-5
3	0.0	0.0	0.0	0.0	8.254039526944487E-5
4	0.0	0.0	0.0	8.254039526944487E-5	8.254039526944487E-5
5	0.0	0.0	0.0	8.254039526944487E-5	8.254039526944487E-5
6	0.0	0.0	0.0	8.254039526944487E-5	5.777827668861141E-4
7	0.0	0.0	0.0	1.6508079053888973E-4	5.777827668861141E-4
8	0.0	0.0	0.0	1.6508079053888973E-4	5.777827668861141E-4
9	0.0	0.0	0.0	1.6508079053888973E-4	5.777827668861141E-4
10	0.0	0.0	0.0	1.6508079053888973E-4	5.777827668861141E-4
11	0.0	0.0	0.0	1.6508079053888973E-4	5.777827668861141E-4
12	0.0	0.0	0.0	1.6508079053888973E-4	0.0011555655337722281
13	0.0	0.0	0.0	1.6508079053888973E-4	0.0011555655337722281
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCCG	45	6.759234E-4	14.778229	9
CGAGCCG	200	0.0	14.727883	15
CGCATCG	145	0.0	14.416021	13
AGGCCCG	205	0.0	14.366291	10
CAACGGA	60	2.5635949E-5	14.252201	14
TCCGCAC	40	0.0052988906	14.241603	3
CGGTCCA	210	0.0	14.024237	10
GGTCGGC	95	4.9003575E-9	13.9992695	11
AACCGCG	70	7.2570783E-6	13.571841	7
TAAGACT	85	2.7044916E-7	13.408848	4
CCGAGCC	230	0.0	13.219434	14
CAAGACG	190	0.0	12.997172	4
TCCAACG	165	0.0	12.672815	18
TCGGCGT	100	1.4384932E-7	12.351908	13
CGCAAGA	185	0.0	12.316553	2
TGATCAA	85	3.9399292E-6	12.294493	10
GATAACG	70	1.0910467E-4	12.213648	11
CGTCTTA	55	0.003063273	12.093277	15
ATAACGA	55	0.0030651707	12.092278	12
CGCGCCA	55	0.0030670695	12.091278	10
>>END_MODULE