FastQCFastQC Report
Thu 2 Feb 2017
SRR4062480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211528
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA27350.2257479810619317No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24840.20503034184930105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT300.00574525629.3330864
TCGTATA655.9316517E-727.07669444
TTAGGAC4900.022.8987123
GATATAC4450.022.7470131
ATACCGT2350.021.531736
GTATAAT1700.020.7108351
GTATTAG3300.020.671611
GACGTGA4650.020.3439147
TAGGACG6200.020.2256364
ATACACT6100.020.196554
ACCGTCG2400.020.1664968
CGTCGTA2100.019.90459310
ATTTCGT2900.019.7239742
GATTTCG2800.019.6426941
AATGCCG2150.019.44169618
CTAGCGG2700.018.74058229
CCAACGA3300.018.66650829
CCGTCGT2250.018.577629
GTAGGAC7850.018.4974233
TAGCGGC2750.018.39984530
Produced by FastQC (version 0.11.5)