##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1211528
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22830838412319	32.0	32.0	32.0	32.0	32.0
2	31.36840502241797	32.0	32.0	32.0	32.0	32.0
3	31.482916614391083	32.0	32.0	32.0	32.0	32.0
4	31.594967677181213	32.0	32.0	32.0	32.0	32.0
5	31.52233790717177	32.0	32.0	32.0	32.0	32.0
6	35.12226956372449	36.0	36.0	36.0	36.0	36.0
7	35.14845963114348	36.0	36.0	36.0	36.0	36.0
8	35.07994780145403	36.0	36.0	36.0	36.0	36.0
9	35.224217682133634	36.0	36.0	36.0	36.0	36.0
10	35.08461958782628	36.0	36.0	36.0	36.0	36.0
11	35.230213416446006	36.0	36.0	36.0	36.0	36.0
12	35.12972873924499	36.0	36.0	36.0	36.0	36.0
13	35.17247558455108	36.0	36.0	36.0	36.0	36.0
14	35.12415313554454	36.0	36.0	36.0	36.0	36.0
15	35.08581724896164	36.0	36.0	36.0	36.0	36.0
16	35.09496189935354	36.0	36.0	36.0	36.0	36.0
17	35.076208721548326	36.0	36.0	36.0	36.0	36.0
18	35.08403107480801	36.0	36.0	36.0	36.0	36.0
19	35.06939831353464	36.0	36.0	36.0	36.0	36.0
20	35.055874895173694	36.0	36.0	36.0	36.0	36.0
21	35.032893172918826	36.0	36.0	36.0	36.0	36.0
22	35.007068759450874	36.0	36.0	36.0	36.0	36.0
23	34.951161673523025	36.0	36.0	36.0	32.0	36.0
24	34.926230347131884	36.0	36.0	36.0	32.0	36.0
25	34.89019733757701	36.0	36.0	36.0	32.0	36.0
26	34.83580074088259	36.0	36.0	36.0	32.0	36.0
27	34.80567927443691	36.0	36.0	36.0	32.0	36.0
28	34.77074157592726	36.0	36.0	36.0	32.0	36.0
29	34.73622070641372	36.0	36.0	36.0	32.0	36.0
30	34.722283760672475	36.0	36.0	36.0	32.0	36.0
31	34.705173136733116	36.0	36.0	36.0	32.0	36.0
32	34.65856175012051	36.0	36.0	36.0	32.0	36.0
33	34.632378285933136	36.0	36.0	36.0	32.0	36.0
34	34.611002799770205	36.0	36.0	36.0	32.0	36.0
35	34.561081543307296	36.0	36.0	36.0	32.0	36.0
36	34.52425944757364	36.0	36.0	36.0	32.0	36.0
37	34.47498365700174	36.0	36.0	36.0	32.0	36.0
38	34.42193246875021	36.0	36.0	36.0	32.0	36.0
39	34.3280378167075	36.0	36.0	36.0	32.0	36.0
40	34.285442020324744	36.0	36.0	36.0	32.0	36.0
41	34.27511786768444	36.0	36.0	36.0	32.0	36.0
42	34.17571281885355	36.0	36.0	36.0	32.0	36.0
43	34.18094753072153	36.0	36.0	36.0	32.0	36.0
44	34.1699085782582	36.0	36.0	36.0	32.0	36.0
45	34.04551029773971	36.0	36.0	36.0	32.0	36.0
46	34.11053149411322	36.0	36.0	36.0	32.0	36.0
47	34.04924690143356	36.0	36.0	36.0	32.0	36.0
48	34.02479100771918	36.0	36.0	36.0	32.0	36.0
49	34.05216057738657	36.0	36.0	36.0	32.0	36.0
50	33.500693339320264	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	26.0
22	120.0
23	366.0
24	961.0
25	2435.0
26	5040.0
27	9389.0
28	15791.0
29	22747.0
30	31722.0
31	43886.0
32	65463.0
33	97806.0
34	211292.0
35	704474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19978899627111	17.143227448976475	11.42119596847492	26.235787586277493
2	15.530996205489616	20.21606505063612	37.516126875484424	26.736811868389836
3	19.19745774953055	24.036400404448937	28.37357875404965	28.392563091970864
4	12.161769536931109	15.72727287734642	35.85701008480214	36.253947500920326
5	13.778715803514242	37.24032791648232	33.962071037565785	15.01888524243765
6	34.74527516192429	35.13808652415184	16.734556421958146	13.382081891965733
7	29.97696295666543	30.314718532892787	20.755459845302664	18.952858665139118
8	28.199018099457874	33.79286322726342	19.439666272673847	18.56845240060486
9	27.26457828461249	14.052914996599336	17.90482762263852	40.777679096149654
10	15.134111634233793	27.487189730654183	32.578116230083005	24.80058240502902
11	37.85533640163496	20.596965154746734	21.85067121849433	19.697027225123975
12	23.95949577723338	23.63065484247991	29.621931973507838	22.787917406778877
13	30.65038529856512	19.370414881042784	24.878830699744455	25.10036912064765
14	23.2630199219498	19.53128611142293	25.1939699288832	32.011724037744074
15	25.47856921177224	27.381538024709297	22.759028268434573	24.380864495083895
16	25.760774823198474	25.48764865525188	24.863148024643262	23.888428496906386
17	23.819094564880054	25.958376529473526	26.218874016943893	24.00365488870253
18	25.057035413131185	24.577475716615712	27.370972854114804	22.9945160161383
19	25.358885638631545	25.874928189856117	25.033346319688853	23.73283985182348
20	25.04019717249622	24.353048381878093	26.599715400717113	24.00703904490858
21	26.004434070033877	24.870741741008047	24.069191962546473	25.055632226411607
22	24.838963688829313	25.400816159428423	24.993231687587905	24.76698846415436
23	23.845590031761542	24.91077383271373	25.286167550399163	25.95746858512556
24	24.203155024068778	25.95944955461203	25.207424013312114	24.62997140800708
25	25.162356957494996	24.69237194683078	24.929510502439893	25.215760593234325
26	23.659626521219483	25.4357307466274	26.014751619442556	24.88989111271056
27	25.203131912758103	25.35393321491538	25.381914408911722	24.061020463414796
28	24.224202824862488	24.79505219854597	25.656526303973166	25.324218672618382
29	23.814389762349695	25.275684920199947	25.986687885050948	24.923237432399418
30	24.640454038206297	25.10672473108339	26.052472580080693	24.200348650629618
31	24.425683929715202	25.051009964276517	25.197271544693972	25.32603456131431
32	24.19869784272423	25.46107064797512	25.39074623120555	24.9494852780951
33	23.992924637317504	24.87197984693709	25.853054985109715	25.28204053063569
34	24.050372752425037	25.715047444219202	26.29027145885196	23.9443083445038
35	25.30358357380102	25.106064407921235	25.855778818153603	23.73457320012414
36	24.117725714964905	25.768698701144338	25.75359380881003	24.359981775080726
37	25.224592415528157	25.35261256859107	25.34510139262155	24.077693623259222
38	24.480490752174113	25.449762613823207	25.032851077317236	25.03689555668544
39	24.856049550650088	24.807928500208	25.54914124972762	24.786880699414294
40	25.63638644752742	25.08303563764106	25.180680925244815	24.099896989586703
41	23.873983927734233	25.0783308351107	26.466907904728576	24.58077733242649
42	25.633662614483526	25.712406151570576	25.525121994704207	23.128809239241686
43	24.56303114744356	24.42609663169155	25.631681644997062	25.379190575867828
44	24.142652914336278	25.16780462358278	26.086809384512783	24.60273307756816
45	24.532738822379674	25.394955791364293	25.451578502519133	24.6207268837369
46	23.975752853430883	24.78068944568989	26.099854398262025	25.143703302617197
47	24.83989213612243	24.522028811574152	26.441672368837015	24.1964066834664
48	24.638968311091446	26.20541993251497	24.705330788888084	24.450280967505496
49	23.809354798238257	25.212128815842473	26.464431692870487	24.51408469304878
50	23.778256695888743	26.646785420382706	24.482079227289198	25.092878656439353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	70.5
4	134.0
5	142.0
6	150.0
7	146.5
8	143.0
9	215.0
10	287.0
11	418.0
12	549.0
13	976.0
14	1403.0
15	1937.0
16	2471.0
17	2846.5
18	3222.0
19	3629.5
20	4037.0
21	4670.5
22	5304.0
23	5923.5
24	6543.0
25	7334.0
26	8125.0
27	11064.0
28	14003.0
29	16140.0
30	18277.0
31	20131.0
32	21985.0
33	26044.0
34	30103.0
35	34792.5
36	39482.0
37	44144.0
38	48806.0
39	51346.0
40	53886.0
41	57911.0
42	61936.0
43	64225.5
44	66515.0
45	75193.0
46	83871.0
47	90497.5
48	97124.0
49	96694.5
50	96265.0
51	90016.0
52	83767.0
53	81001.0
54	78235.0
55	78837.5
56	79440.0
57	76795.0
58	74150.0
59	67685.5
60	61221.0
61	54300.0
62	47379.0
63	41333.5
64	35288.0
65	30388.0
66	25488.0
67	22466.5
68	19445.0
69	17821.0
70	16197.0
71	12577.5
72	8958.0
73	7943.0
74	6928.0
75	5140.0
76	3352.0
77	2943.5
78	2535.0
79	2207.0
80	1879.0
81	1501.0
82	1123.0
83	944.5
84	766.0
85	613.5
86	461.0
87	322.5
88	184.0
89	122.5
90	61.0
91	37.5
92	14.0
93	11.5
94	9.0
95	8.0
96	7.0
97	6.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014444569172152852
2	0.0033841562060472393
3	2.476211858083346E-4
4	1.6508079053888973E-4
5	0.0
6	4.1270197634722433E-4
7	8.254039526944487E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.3016158107777947E-4
47	8.254039526944487E-5
48	0.0
49	0.0
50	8.254039526944487E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1211528.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.355512841228695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09950333353085	46.158920825146716
2	12.818448637644098	14.960542881573414
3	3.7347406944764785	6.538281256655444
4	1.4952632229025025	3.4902740882041594
5	0.7946207837505606	2.318525167503152
6	0.4625703118889218	1.6196116665243083
7	0.30075447056667215	1.2285476958447261
8	0.19236511883553598	0.8980452129929283
9	0.16214075836362918	0.851562639609779
>10	0.7938121639798734	8.388521281152457
>50	0.07442205677565146	3.0328795164235682
>100	0.06537630740478705	7.677758454669911
>500	0.004985116567035289	1.9584827326955299
>1k	9.970233134070578E-4	0.8780465810039291
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2735	0.2257479810619317	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2484	0.20503034184930105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6508079053888973E-4
2	0.0	0.0	0.0	0.0	1.6508079053888973E-4
3	0.0	0.0	0.0	0.0	1.6508079053888973E-4
4	0.0	0.0	0.0	0.0	1.6508079053888973E-4
5	0.0	0.0	0.0	0.0	3.3016158107777947E-4
6	0.0	0.0	0.0	0.0	3.3016158107777947E-4
7	0.0	0.0	0.0	0.0	3.3016158107777947E-4
8	0.0	0.0	0.0	0.0	3.3016158107777947E-4
9	0.0	0.0	0.0	0.0	3.3016158107777947E-4
10	0.0	0.0	0.0	0.0	3.3016158107777947E-4
11	0.0	0.0	0.0	0.0	4.1270197634722433E-4
12	0.0	0.0	0.0	0.0	9.079443479638935E-4
13	0.0	0.0	0.0	0.0	0.0010730251385027833
14	0.0	0.0	0.0	8.254039526944487E-5	0.0010730251385027833
15	0.0	0.0	0.0	8.254039526944487E-5	0.0011555655337722281
16	0.0	0.0	0.0	1.6508079053888973E-4	0.0011555655337722281
17	0.0	0.0	0.0	1.6508079053888973E-4	0.0011555655337722281
18	0.0	0.0	0.0	4.1270197634722433E-4	0.0011555655337722281
19	0.0	0.0	0.0	6.603231621555589E-4	0.0011555655337722281
20	0.0	0.0	0.0	9.904847432333384E-4	0.0014857271148500076
21	0.0	0.0	0.0	0.0015682675101194525	0.0017333483006583422
22	0.0	0.0	0.0	0.0025587522533527906	0.0017333483006583422
23	0.0	0.0	0.0	0.004869883320897247	0.0017333483006583422
24	0.0	0.0	0.0	0.0074286355742500376	0.0017333483006583422
25	0.0	0.0	0.0	0.009244524270177825	0.0017333483006583422
26	0.0	0.0	0.0	0.011803276523530616	0.0017333483006583422
27	8.254039526944487E-5	0.0	0.0	0.014939811543769521	0.001898429091197232
28	8.254039526944487E-5	0.0	0.0	0.019974775655205657	0.001898429091197232
29	8.254039526944487E-5	0.0	0.0	0.028393895972689032	0.001980969486466677
30	8.254039526944487E-5	0.0	0.0	0.04399403067861411	0.001980969486466677
31	8.254039526944487E-5	0.0	0.0	0.07701018878639206	0.0020635098817361214
32	8.254039526944487E-5	0.0	0.0	0.11712482088734226	0.0020635098817361214
33	8.254039526944487E-5	0.0	0.0	0.1567442106166758	0.0020635098817361214
34	8.254039526944487E-5	0.0	0.0	0.20387477631552883	0.0021460502770055665
35	8.254039526944487E-5	0.0	0.0	0.25802127561228466	0.0021460502770055665
36	8.254039526944487E-5	0.0	0.0	0.3323901717500545	0.0021460502770055665
37	8.254039526944487E-5	0.0	0.0	0.44637845761715783	0.0021460502770055665
38	8.254039526944487E-5	0.0	0.0	0.592062255267728	0.0021460502770055665
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTT	30	0.005745256	29.333086	4
TCGTATA	65	5.9316517E-7	27.076694	44
TTAGGAC	490	0.0	22.898712	3
GATATAC	445	0.0	22.747013	1
ATACCGT	235	0.0	21.53173	6
GTATAAT	170	0.0	20.710835	1
GTATTAG	330	0.0	20.67161	1
GACGTGA	465	0.0	20.343914	7
TAGGACG	620	0.0	20.225636	4
ATACACT	610	0.0	20.19655	4
ACCGTCG	240	0.0	20.166496	8
CGTCGTA	210	0.0	19.904593	10
ATTTCGT	290	0.0	19.72397	42
GATTTCG	280	0.0	19.64269	41
AATGCCG	215	0.0	19.441696	18
CTAGCGG	270	0.0	18.740582	29
CCAACGA	330	0.0	18.666508	29
CCGTCGT	225	0.0	18.57762	9
GTAGGAC	785	0.0	18.497423	3
TAGCGGC	275	0.0	18.399845	30
>>END_MODULE