FastQCFastQC Report
Thu 2 Feb 2017
SRR4062479_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062479_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337206
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG39260.29359724679667903No Hit
GTATCAACGCAGAGTACTTTTTTTT36950.2763224215266758No Hit
TCCATGTACTCTGCGTTGATACCAC33680.2518684480925153No Hit
GTACTTTTTTTTTTTTTTTTTTTTT27910.20871877631419541No Hit
TATCAACGCAGAGTACTTTTTTTTT24440.1827691470125022No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22110.16534475615574562No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17160.12832727343431005No Hit
GAGTACATGGAAGCAGTGGTATCAA17150.12825249064093341No Hit
CATGTACTCTGCGTTGATACCACTG15020.11232375565170961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC755.8184014E-815.20527618
GATATAC1650.014.3838451
CGCGCTA707.2403363E-613.57512116
ACGCTTA801.9910276E-613.06703317
CTCGACG905.3901385E-712.66916513
TAACACC550.003072996212.0883124
CATTATA951.0463482E-611.9933812
GTCCTAA2000.011.86667251
CGAACCT2350.011.72659416
CGTTCAA658.008864E-411.694612514
CGTCTAT658.0694695E-411.6841091
GACGCTT907.4356885E-611.61427116
CGACGCT907.4449963E-611.61296615
GCATCGC1900.011.50226714
CGTTATT1001.9415638E-611.3937122
TTTCGGA1604.5474735E-1111.28516517
CGACCAT1604.5474735E-1111.28220610
TTCGGAA1701.0913936E-1111.18034918
CCGAATC600.005864673811.08593419
GACCATA1803.6379788E-1211.083858511
Produced by FastQC (version 0.11.5)