##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062479_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337206
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.087978217267946	32.0	32.0	32.0	32.0	32.0
2	30.827110407820484	32.0	32.0	32.0	32.0	32.0
3	30.807512828988205	32.0	32.0	32.0	32.0	32.0
4	30.854370231662138	32.0	32.0	32.0	32.0	32.0
5	30.70909717724868	32.0	32.0	32.0	32.0	32.0
6	34.292394739479185	36.0	36.0	36.0	32.0	36.0
7	34.18360073167485	36.0	36.0	36.0	32.0	36.0
8	34.195429126103235	36.0	36.0	36.0	32.0	36.0
9	34.311711135008366	36.0	36.0	36.0	32.0	36.0
10	34.05194412827941	36.0	36.0	36.0	32.0	36.0
11	34.31002553084566	36.0	36.0	36.0	32.0	36.0
12	34.126737391247126	36.0	36.0	36.0	32.0	36.0
13	34.2255950092955	36.0	36.0	36.0	32.0	36.0
14	34.13779552290372	36.0	36.0	36.0	32.0	36.0
15	34.10012967336372	36.0	36.0	36.0	32.0	36.0
16	34.09434597212397	36.0	36.0	36.0	32.0	36.0
17	34.02616500374662	36.0	36.0	36.0	32.0	36.0
18	34.04524508564873	36.0	36.0	36.0	32.0	36.0
19	34.02957360346873	36.0	36.0	36.0	32.0	36.0
20	34.013823599355675	36.0	36.0	36.0	32.0	36.0
21	33.989093677413955	36.0	36.0	36.0	32.0	36.0
22	33.95326598893514	36.0	36.0	36.0	32.0	36.0
23	33.91395940490845	36.0	36.0	36.0	32.0	36.0
24	33.89441940882706	36.0	36.0	36.0	32.0	36.0
25	33.43617812064858	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	51.0
5	134.0
6	230.0
7	67.0
8	178.0
9	236.0
10	151.0
11	43.0
12	107.0
13	72.0
14	196.0
15	305.0
16	435.0
17	714.0
18	999.0
19	1414.0
20	2204.0
21	3065.0
22	4323.0
23	5997.0
24	8103.0
25	11196.0
26	15413.0
27	20351.0
28	27402.0
29	36781.0
30	47744.0
31	65185.0
32	94220.0
33	136312.0
34	305519.0
35	548046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.162115409209314	17.722718444278414	11.160471556791062	25.95469458972121
2	16.52471168393335	20.58061819033011	37.09702870484556	25.797641420890976
3	19.198691533708562	24.445719140432775	28.438351164950816	27.917238160907846
4	12.566575941636804	15.541504270359093	36.407363611409664	35.48455617659444
5	14.319399849782904	36.70795641908774	33.81403302980702	15.158610701322328
6	33.19045559766987	35.673894599176705	17.36312392729471	13.772525875858726
7	29.167570900676942	30.923456154503537	21.39315396323896	18.515818981580566
8	27.73203716067531	32.6844486226419	20.332535949630103	19.250978267052684
9	27.352243703945454	14.439979759206418	19.039670158902204	39.16810637794592
10	15.538573153812694	26.888264797687384	32.07203187107817	25.50113017742176
11	37.073282855395156	21.490559158092083	22.378901988794826	19.057255997717938
12	24.036629098552464	24.19814877632183	29.370073256699474	22.395148868426233
13	29.193186948614606	19.956379580013074	25.614156196443133	25.236277274929193
14	23.150512925912697	19.974422963027855	25.6027188330402	31.272345278019248
15	24.899676569238142	27.201575227599424	23.21349425011979	24.685253953042647
16	25.191246030213055	26.09518205244536	24.489299355999865	24.224272561341724
17	23.445619131762978	25.767082466044716	26.254511148714414	24.53278725347789
18	24.252954896873444	24.92506523798292	27.396433532136022	23.42554633300761
19	24.894792175233555	25.556365934530035	26.103596385343693	23.445245504892725
20	24.972334324156364	24.545781265208635	26.4171639500146	24.0647204606204
21	25.879501400597977	24.646106592207715	24.902489777244643	24.57190222994966
22	24.88552534020356	25.19206428854954	25.641724646690083	24.280685724556818
23	23.803868150104055	24.787118447587815	26.050741107126356	25.35827229518177
24	23.798605411816858	25.687784174299562	25.991876883263142	24.521733530620434
25	24.185218659044942	25.08946214884375	25.932828696177634	24.792490495933674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168.0
1	168.0
2	527.5
3	887.0
4	887.0
5	887.0
6	2563.0
7	4239.0
8	4239.0
9	4239.0
10	4429.0
11	4619.0
12	4619.0
13	4619.0
14	4666.0
15	4713.0
16	4713.0
17	4713.0
18	7979.0
19	11245.0
20	11245.0
21	11245.0
22	17703.5
23	24162.0
24	24162.0
25	24162.0
26	35327.5
27	46493.0
28	46493.0
29	46493.0
30	57136.5
31	67780.0
32	67780.0
33	67780.0
34	80012.5
35	92245.0
36	92245.0
37	92245.0
38	102481.0
39	112717.0
40	112717.0
41	112717.0
42	126345.0
43	139973.0
44	139973.0
45	139973.0
46	160611.0
47	181249.0
48	181249.0
49	181249.0
50	174623.5
51	167998.0
52	167998.0
53	167998.0
54	153649.0
55	139300.0
56	139300.0
57	139300.0
58	126733.0
59	114166.0
60	114166.0
61	114166.0
62	101690.5
63	89215.0
64	89215.0
65	89215.0
66	75323.0
67	61431.0
68	61431.0
69	61431.0
70	47507.0
71	33583.0
72	33583.0
73	33583.0
74	26595.0
75	19607.0
76	19607.0
77	19607.0
78	15890.0
79	12173.0
80	12173.0
81	12173.0
82	8746.5
83	5320.0
84	5320.0
85	5320.0
86	3858.0
87	2396.0
88	2396.0
89	2396.0
90	1593.5
91	791.0
92	791.0
93	791.0
94	474.5
95	158.0
96	158.0
97	158.0
98	368.0
99	578.0
100	578.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007702627717793668
2	0.00336522570194869
3	0.005085229949611354
4	0.014881775881950873
5	0.035447044060526206
6	0.04726272541403494
7	0.06730451403897379
8	0.08749586825066594
9	0.09646980345586245
10	0.11778289956820415
11	0.11763333398145087
12	0.1319916303097653
13	0.11912898984898362
14	0.12212030158404914
15	0.1141185426927489
16	0.11157592771794324
17	0.12159682203041267
18	0.12795335946742686
19	0.13116901958262228
20	0.12084899409664629
21	0.12750466270716704
22	0.1477707997122358
23	0.13752555701963648
24	0.13146815075612883
25	0.1290003185746998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1337206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.21870784455784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38168284632322	40.40771489072385
2	13.757022509174647	14.367478784351938
3	4.145398785370721	6.494021042173759
4	1.6494963075551254	3.4453826309959212
5	0.822751158990867	2.1481501200057727
6	0.48212167372934545	1.5105462495601159
7	0.302168554381783	1.1045196022752304
8	0.21934072702718632	0.9162951474436444
9	0.14713997266583634	0.6915113320404181
>10	0.8769913116499892	8.717319657904962
>50	0.10555942437615232	3.890632958758449
>100	0.09899278715466871	10.623978076889928
>500	0.008177654066119103	2.8197235931416693
>1k	0.0031562875342915834	2.8627259137342436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	3926	0.29359724679667903	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3695	0.2763224215266758	No Hit
TCCATGTACTCTGCGTTGATACCAC	3368	0.2518684480925153	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2791	0.20871877631419541	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2444	0.1827691470125022	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2211	0.16534475615574562	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1716	0.12832727343431005	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1715	0.12825249064093341	No Hit
CATGTACTCTGCGTTGATACCACTG	1502	0.11232375565170961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.478279337663756E-5	0.0
8	0.0	0.0	0.0	7.478279337663756E-5	0.0
9	0.0	0.0	0.0	1.495655867532751E-4	0.0
10	0.0	0.0	0.0	1.495655867532751E-4	0.0
11	0.0	0.0	0.0	1.495655867532751E-4	0.0
12	0.0	0.0	0.0	2.2434838012991266E-4	0.0
13	0.0	0.0	0.0	2.2434838012991266E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	75	5.8184014E-8	15.205276	18
GATATAC	165	0.0	14.383845	1
CGCGCTA	70	7.2403363E-6	13.575121	16
ACGCTTA	80	1.9910276E-6	13.067033	17
CTCGACG	90	5.3901385E-7	12.669165	13
TAACACC	55	0.0030729962	12.088312	4
CATTATA	95	1.0463482E-6	11.993381	2
GTCCTAA	200	0.0	11.8666725	1
CGAACCT	235	0.0	11.726594	16
CGTTCAA	65	8.008864E-4	11.6946125	14
CGTCTAT	65	8.0694695E-4	11.684109	1
GACGCTT	90	7.4356885E-6	11.614271	16
CGACGCT	90	7.4449963E-6	11.612966	15
GCATCGC	190	0.0	11.502267	14
CGTTATT	100	1.9415638E-6	11.393712	2
TTTCGGA	160	4.5474735E-11	11.285165	17
CGACCAT	160	4.5474735E-11	11.282206	10
TTCGGAA	170	1.0913936E-11	11.180349	18
CCGAATC	60	0.0058646738	11.085934	19
GACCATA	180	3.6379788E-12	11.0838585	11
>>END_MODULE