FastQCFastQC Report
Thu 2 Feb 2017
SRR4062479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1337206
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30710.22965795845965395No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC25240.1887517704826332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG1650.023.99985713
ACGCTTA1800.023.22208417
TCGACGC2000.021.9998714
CATACGT654.9327366E-420.30757331
GACGCTT1950.020.30757116
TACCGTC3300.019.9998827
CCTCGAC2050.019.3169612
ATACCGT3350.019.0446626
CGCTTAC2100.018.8570318
CGTGCAC2300.018.17380336
GATATAC2600.017.7731131
AGCGCGT2250.017.59989532
TACGTTG1002.483459E-517.59989533
CGTCGTA3100.017.03215610
CAAGACG5300.017.0187684
TAACGCC4400.016.9998994
ACCGTCG3650.016.8766128
ATAACGC4600.016.739033
TACGCTA3700.016.6485489
AAGACGG5450.016.550365
Produced by FastQC (version 0.11.5)