##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1337206
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.203303754245795	32.0	32.0	32.0	32.0	32.0
2	31.320104755736963	32.0	32.0	32.0	32.0	32.0
3	31.430156610125888	32.0	32.0	32.0	32.0	32.0
4	31.54809356224845	32.0	32.0	32.0	32.0	32.0
5	31.462082880274245	32.0	32.0	32.0	32.0	32.0
6	35.03153515613899	36.0	36.0	36.0	36.0	36.0
7	35.04204064295254	36.0	36.0	36.0	36.0	36.0
8	34.96827040859823	36.0	36.0	36.0	36.0	36.0
9	35.116199000004485	36.0	36.0	36.0	36.0	36.0
10	34.98032763837434	36.0	36.0	36.0	36.0	36.0
11	35.12884551819241	36.0	36.0	36.0	36.0	36.0
12	35.03066468442409	36.0	36.0	36.0	36.0	36.0
13	35.07813904514338	36.0	36.0	36.0	36.0	36.0
14	35.038013589529214	36.0	36.0	36.0	36.0	36.0
15	34.997761750994236	36.0	36.0	36.0	36.0	36.0
16	35.01392006915913	36.0	36.0	36.0	36.0	36.0
17	34.992817860524106	36.0	36.0	36.0	36.0	36.0
18	35.00536716108064	36.0	36.0	36.0	36.0	36.0
19	34.97465835480846	36.0	36.0	36.0	36.0	36.0
20	34.96088485992435	36.0	36.0	36.0	32.0	36.0
21	34.9506650433815	36.0	36.0	36.0	32.0	36.0
22	34.93916868455571	36.0	36.0	36.0	32.0	36.0
23	34.90105189477163	36.0	36.0	36.0	32.0	36.0
24	34.87510600460961	36.0	36.0	36.0	32.0	36.0
25	34.858155736662866	36.0	36.0	36.0	32.0	36.0
26	34.822402830977424	36.0	36.0	36.0	32.0	36.0
27	34.80559016337049	36.0	36.0	36.0	32.0	36.0
28	34.78392633595721	36.0	36.0	36.0	32.0	36.0
29	34.7649681500083	36.0	36.0	36.0	32.0	36.0
30	34.743418740268886	36.0	36.0	36.0	32.0	36.0
31	34.7434942708902	36.0	36.0	36.0	32.0	36.0
32	34.70475753174904	36.0	36.0	36.0	32.0	36.0
33	34.68191064054454	36.0	36.0	36.0	32.0	36.0
34	34.66553470445092	36.0	36.0	36.0	32.0	36.0
35	34.629994181898674	36.0	36.0	36.0	32.0	36.0
36	34.600665118164294	36.0	36.0	36.0	32.0	36.0
37	34.59216156672944	36.0	36.0	36.0	32.0	36.0
38	34.5455434689943	36.0	36.0	36.0	32.0	36.0
39	34.536826786598326	36.0	36.0	36.0	32.0	36.0
40	34.51474567119801	36.0	36.0	36.0	32.0	36.0
41	34.4823265824413	36.0	36.0	36.0	32.0	36.0
42	34.445916336002085	36.0	36.0	36.0	32.0	36.0
43	34.43157972668385	36.0	36.0	36.0	32.0	36.0
44	34.415183599236016	36.0	36.0	36.0	32.0	36.0
45	34.34472100783275	36.0	36.0	36.0	32.0	36.0
46	34.35982563643896	36.0	36.0	36.0	32.0	36.0
47	34.3248317761063	36.0	36.0	36.0	32.0	36.0
48	34.27652956986433	36.0	36.0	36.0	32.0	36.0
49	34.26368113813429	36.0	36.0	36.0	32.0	36.0
50	33.695974292666946	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	6.0
21	27.0
22	93.0
23	340.0
24	890.0
25	2082.0
26	4344.0
27	8333.0
28	13947.0
29	22109.0
30	33177.0
31	48721.0
32	72442.0
33	119648.0
34	251356.0
35	759690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84807032529112	18.287612370202552	11.562939654611451	26.301377649894874
2	16.091552587396375	21.133974744887464	36.634197232183254	26.140275435532907
3	18.705849976293784	24.85099483847616	28.71480898173948	27.728346203490574
4	12.550889767006382	16.058133239206583	36.329385793042796	35.06159120074424
5	14.110914847824493	36.97949306240026	33.599834281329876	15.30975780844537
6	33.44426180715197	36.0402124442304	16.961586840542687	13.553938908074944
7	29.388815186291417	31.015490507820036	21.212064558489864	18.38362974739868
8	27.98230040846362	32.861728110702465	19.992357198516906	19.16361428231701
9	27.59769250212757	14.233708194548933	18.656437377636653	39.51216192568685
10	15.713734458265966	26.989558826388755	31.914304901413843	25.382401813931438
11	37.43170461394879	21.415174625300814	22.124190289304714	19.028930471445683
12	24.187671906946274	24.21579023725589	29.098358816816557	22.498179038981277
13	29.518114636039623	19.905235244233126	25.402069688589492	25.174580431137763
14	23.353918543590144	19.915704835305853	25.265217176710248	31.46515944439376
15	25.13786207958983	27.346272750795315	22.83088768671394	24.684977482900912
16	25.598972783550177	26.062177405725073	24.197019756118355	24.141830054606395
17	23.68490718707514	25.950751043593883	25.7848828078845	24.57945896144648
18	24.528831010330496	25.2231144640392	26.80364880205443	23.444405723575873
19	25.23597710449998	25.7976706655519	25.47640378520587	23.489948444742247
20	25.462045488877553	24.652521750575453	25.72393483128254	24.161497929264453
21	26.289143183623164	24.724612363390534	24.36086885640657	24.625375596579737
22	25.162241270230616	25.30672162703428	25.15767951983464	24.373357582900464
23	24.078862942583267	24.898258009611084	25.509457779878343	25.513421267927306
24	24.202628465621604	25.95254583063492	25.3201077470487	24.52471795669478
25	24.707187972533777	25.067715819402547	25.372680050792475	24.852416157271207
26	23.799773557701656	25.78420976274411	25.91829531126842	24.497721368285813
27	25.029427029193705	25.61243368635797	25.232985792764918	24.125153491683406
28	24.046481993051184	25.28757723192986	25.80851417059152	24.85742660442744
29	23.981495745606885	25.673680794133443	25.79445500543671	24.550368454822966
30	24.54722757750115	25.595981471815115	25.763195797805277	24.093595152878464
31	24.28204779218759	25.59358842242706	25.084018468358654	25.040345317026695
32	24.1363709106899	25.876416946977503	25.137263817242818	24.849948325089777
33	24.039377627680402	25.21443966000751	25.615798912059923	25.130383800252165
34	24.267913844239406	25.644590287509928	25.924502283118684	24.162993585131982
35	25.240164940929073	25.38673921594728	25.393768798524686	23.979327044598964
36	24.02202801961702	26.05731652415559	25.317041652520256	24.603613803707134
37	25.075044533153456	25.863030826963083	25.0406444482002	24.021280191683257
38	24.251312064109793	25.651993784054216	25.291615502772196	24.805078649063795
39	24.453150823433337	25.57048053927368	25.130907279805804	24.845461357487178
40	25.07093147951774	25.638009401692784	25.293185941433105	23.997873177356368
41	23.855187607593745	25.793931525883075	26.002650302197267	24.348230564325917
42	25.381205289237407	26.05522260594104	25.107724613859045	23.4558474909625
43	24.425481189883982	25.149827326530094	25.57908056051199	24.84561092307393
44	24.155664871381074	25.61647195720031	25.43101062962625	24.796852541792365
45	24.42570553826411	26.020373824227534	25.309264242009082	24.244656395499273
46	24.17084577843653	25.37858789146923	25.415081894637026	25.035484435457217
47	24.499067458566593	25.084616730705665	25.93168143128284	24.484634379444902
48	24.82669087634964	26.206283848561853	24.7733707446721	24.193654530416406
49	23.96272526446935	25.70852957584695	25.81950724121788	24.509237918465814
50	23.909704263965313	26.390623434235266	24.830803929985358	24.868868371814067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	13.0
2	13.0
3	43.0
4	73.0
5	86.0
6	99.0
7	126.5
8	154.0
9	200.0
10	246.0
11	386.0
12	526.0
13	892.5
14	1259.0
15	1871.5
16	2484.0
17	3196.5
18	3909.0
19	4454.0
20	4999.0
21	6297.5
22	7596.0
23	8507.0
24	9418.0
25	10829.0
26	12240.0
27	14785.0
28	17330.0
29	20047.0
30	22764.0
31	24968.5
32	27173.0
33	30791.5
34	34410.0
35	39577.5
36	44745.0
37	51344.0
38	57943.0
39	60708.0
40	63473.0
41	68018.0
42	72563.0
43	73049.5
44	73536.0
45	81526.0
46	89516.0
47	94801.5
48	100087.0
49	99739.0
50	99391.0
51	93203.0
52	87015.0
53	84021.5
54	81028.0
55	81447.0
56	81866.0
57	80103.5
58	78341.0
59	72143.5
60	65946.0
61	58005.5
62	50065.0
63	44239.5
64	38414.0
65	33119.5
66	27825.0
67	25617.5
68	23410.0
69	21802.5
70	20195.0
71	16172.5
72	12150.0
73	10870.5
74	9591.0
75	7321.0
76	5051.0
77	4439.5
78	3828.0
79	3282.5
80	2737.0
81	2132.5
82	1528.0
83	1274.5
84	1021.0
85	863.5
86	706.0
87	531.5
88	357.0
89	229.5
90	102.0
91	70.0
92	38.0
93	25.5
94	13.0
95	11.0
96	9.0
97	6.5
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016377431749483626
2	0.0038139224622085154
3	2.991311735065502E-4
4	1.495655867532751E-4
5	0.0
6	5.982623470131005E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1337206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94254179429935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48855545264254	42.57418196536696
2	13.894603464399188	15.268096631159377
3	4.13157123888195	6.8099707640519
4	1.6223018827405855	3.565335559817805
5	0.8027984765535132	2.205389442522062
6	0.44982134607291163	1.482859686394736
7	0.2803866870913426	1.078361009185686
8	0.19708116007497647	0.8662511899430717
9	0.1389426689753761	0.6870477057471991
>10	0.8080897023614919	8.420034727552162
>50	0.09032593360109335	3.458380678366883
>100	0.08732269177584903	9.788185985417588
>500	0.006286126035703275	2.3336888753362666
>1k	0.0019131687934749097	1.4622157791382082
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3071	0.22965795845965395	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2524	0.1887517704826332	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.495655867532751E-4	0.0
9	0.0	0.0	0.0	2.2434838012991266E-4	0.0
10	7.478279337663756E-5	0.0	0.0	2.2434838012991266E-4	0.0
11	7.478279337663756E-5	0.0	0.0	2.2434838012991266E-4	0.0
12	7.478279337663756E-5	0.0	0.0	3.739139668831878E-4	0.0
13	7.478279337663756E-5	0.0	0.0	3.739139668831878E-4	0.0
14	7.478279337663756E-5	0.0	0.0	3.739139668831878E-4	0.0
15	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
16	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
17	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
18	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
19	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
20	7.478279337663756E-5	0.0	0.0	4.486967602598253E-4	0.0
21	7.478279337663756E-5	0.0	0.0	5.234795536364629E-4	0.0
22	1.495655867532751E-4	0.0	0.0	5.982623470131005E-4	0.0
23	1.495655867532751E-4	0.0	0.0	7.478279337663756E-4	0.0
24	1.495655867532751E-4	0.0	0.0	0.0010469591072729259	0.0
25	1.495655867532751E-4	0.0	0.0	0.0012713074874028385	0.0
26	1.495655867532751E-4	0.0	0.0	0.0014956558675327512	0.0
27	1.495655867532751E-4	0.0	0.0	0.0019443526277925765	0.0
28	1.495655867532751E-4	0.0	0.0	0.0035147912887019653	0.0
29	1.495655867532751E-4	0.0	0.0	0.007179148164157205	0.0
30	1.495655867532751E-4	0.0	0.0	0.012114812527015285	0.0
31	1.495655867532751E-4	0.0	0.0	0.025426149748056768	0.0
32	1.495655867532751E-4	0.0	0.0	0.045393155579618996	0.0
33	1.495655867532751E-4	0.0	0.0	0.06528537861780459	0.0
34	1.495655867532751E-4	0.0	0.0	0.09355327451417358	0.0
35	1.495655867532751E-4	0.0	0.0	0.1298229293018428	0.0
36	1.495655867532751E-4	0.0	0.0	0.17910479013704694	0.0
37	1.495655867532751E-4	0.0	0.0	0.2616649940248548	0.0
38	1.495655867532751E-4	0.0	0.0	0.36748264665279695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	165	0.0	23.999857	13
ACGCTTA	180	0.0	23.222084	17
TCGACGC	200	0.0	21.99987	14
CATACGT	65	4.9327366E-4	20.307573	31
GACGCTT	195	0.0	20.307571	16
TACCGTC	330	0.0	19.999882	7
CCTCGAC	205	0.0	19.31696	12
ATACCGT	335	0.0	19.044662	6
CGCTTAC	210	0.0	18.85703	18
CGTGCAC	230	0.0	18.173803	36
GATATAC	260	0.0	17.773113	1
AGCGCGT	225	0.0	17.599895	32
TACGTTG	100	2.483459E-5	17.599895	33
CGTCGTA	310	0.0	17.032156	10
CAAGACG	530	0.0	17.018768	4
TAACGCC	440	0.0	16.999899	4
ACCGTCG	365	0.0	16.876612	8
ATAACGC	460	0.0	16.73903	3
TACGCTA	370	0.0	16.648548	9
AAGACGG	545	0.0	16.55036	5
>>END_MODULE