FastQCFastQC Report
Thu 2 Feb 2017
SRR4062478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493209
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37670.1510904220223816No Hit
GCGCAAGACGGACCAGAGCGAAAGC25790.10344098709735125No Hit
GTCTTGCGCCGGTCCAAGAATTTCA25670.1029596796738661No Hit
GTACATGGAAGCAGTGGTATCAACG25520.10235804539450964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA307.7188056E-419.00208910
CGAACGA1750.013.57401116
AAGACGG5200.012.0563545
CGCGTAA1459.640644E-1111.794410
TCGCGTA1459.640644E-1111.79449
CGCATCG4400.011.66107613
GGTTCTA4900.011.63463313
TCCATTA5000.011.5905758
CGGTCCA4600.011.56648910
CAAGACG5050.011.4737434
CGACCAT3400.011.45714210
CGGTTCT5150.011.4383812
CGCAAGA4750.011.3941582
ACGAACG2100.011.31122115
ACGGTAT3700.011.2985399
CGCCAGT4550.011.27687118
GTCTTAC1553.1650416E-1011.0248341
GCTCGTA1752.0008883E-1110.8583369
TCGCCAG4950.010.74973617
ATCGCCA5050.010.72481316
Produced by FastQC (version 0.11.5)