##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062478_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2493209
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109662286635416	32.0	32.0	32.0	32.0	32.0
2	30.629764692811552	32.0	32.0	32.0	32.0	32.0
3	30.6158829845392	32.0	32.0	32.0	32.0	32.0
4	30.720035103354753	32.0	32.0	32.0	32.0	32.0
5	30.430245920017136	32.0	32.0	32.0	32.0	32.0
6	34.10014082253032	36.0	36.0	36.0	32.0	36.0
7	33.972311587195456	36.0	36.0	36.0	32.0	36.0
8	33.955057518242555	36.0	36.0	36.0	32.0	36.0
9	34.18584563107225	36.0	36.0	36.0	32.0	36.0
10	33.731288873094876	36.0	36.0	36.0	32.0	36.0
11	34.16488709931658	36.0	36.0	36.0	32.0	36.0
12	33.87010074165463	36.0	36.0	36.0	32.0	36.0
13	34.02911749476277	36.0	36.0	36.0	32.0	36.0
14	33.901491210724814	36.0	36.0	36.0	32.0	36.0
15	33.825101305185406	36.0	36.0	36.0	32.0	36.0
16	33.82718576741862	36.0	36.0	36.0	32.0	36.0
17	33.73645370283839	36.0	36.0	36.0	32.0	36.0
18	33.7549539569286	36.0	36.0	36.0	32.0	36.0
19	33.746938583969495	36.0	36.0	36.0	32.0	36.0
20	33.74764410043442	36.0	36.0	36.0	32.0	36.0
21	33.729323935538496	36.0	36.0	36.0	32.0	36.0
22	33.666802502317296	36.0	36.0	36.0	27.0	36.0
23	33.627463241148256	36.0	36.0	36.0	27.0	36.0
24	33.59500025870274	36.0	36.0	36.0	27.0	36.0
25	33.12108852486895	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	11.0
4	108.0
5	214.0
6	422.0
7	133.0
8	403.0
9	429.0
10	309.0
11	87.0
12	198.0
13	140.0
14	321.0
15	414.0
16	693.0
17	932.0
18	1364.0
19	2062.0
20	3397.0
21	5254.0
22	8369.0
23	12948.0
24	18806.0
25	26420.0
26	36468.0
27	47015.0
28	62195.0
29	82141.0
30	103347.0
31	139899.0
32	195644.0
33	272326.0
34	563743.0
35	906996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69990015952815	17.262694360998918	10.787424031589541	26.249981447883393
2	17.206924628880163	19.76908544680783	36.45477772919984	26.56921219511217
3	19.347953832114957	23.34414327687695	28.083230548619674	29.224672342388413
4	12.83864256954972	14.995204297719445	35.5210908608075	36.64506227192334
5	14.854876502591921	36.28833715835594	33.507839958914445	15.3489463801377
6	34.803781910168	34.89936494662679	16.608134429394955	13.688718713810264
7	30.620405496306734	30.248229626050144	20.52963520102233	18.60172967662079
8	28.363984380551244	32.54223108257457	19.396811798649296	19.696972738224897
9	27.232601508347702	14.391612350224126	18.569779649551048	39.80600649187712
10	15.9592098822148	26.446232869423515	31.54817001450881	26.046387233852876
11	37.68721267552694	21.110859459581015	21.909384567443148	19.292543297448898
12	24.714625488877804	23.690361727404387	28.432185248641908	23.162827535075902
13	29.3456034362285	19.328223110692754	25.244280314773437	26.081893138305308
14	23.414183974497472	19.693177720145247	24.79039044924192	32.10224785611536
15	25.262040322668078	27.07201887597822	22.109445176823762	25.55649562452994
16	26.068859122008647	25.63406760951803	23.49673083201058	24.80034243646274
17	24.22761165109367	25.83413283236303	25.0482610946303	24.889994421913006
18	25.013494322745643	24.783126955873563	26.008620301887277	24.194758419493514
19	25.65543372004572	25.18504922731154	25.060504972532794	24.09901208010995
20	25.562186920976988	24.530537076346906	24.90307952899904	25.004196473677066
21	26.52197276135827	24.312688554822667	24.2450176546752	24.920321029143867
22	25.649340737384353	24.601879613472544	24.67506852924825	25.073711119894853
23	24.438298260320174	24.411989985986327	25.33390421619216	25.81580753750134
24	24.760751137807667	25.16551025030965	25.014137250208446	25.05960136167424
25	24.892135218639293	24.656063661406396	25.15812737984267	25.293673740111643
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	219.0
1	219.0
2	652.0
3	1085.0
4	1085.0
5	1085.0
6	2845.0
7	4605.0
8	4605.0
9	4605.0
10	5059.5
11	5514.0
12	5514.0
13	5514.0
14	6592.0
15	7670.0
16	7670.0
17	7670.0
18	12367.5
19	17065.0
20	17065.0
21	17065.0
22	27029.5
23	36994.0
24	36994.0
25	36994.0
26	54451.5
27	71909.0
28	71909.0
29	71909.0
30	88697.5
31	105486.0
32	105486.0
33	105486.0
34	129927.5
35	154369.0
36	154369.0
37	154369.0
38	175452.0
39	196535.0
40	196535.0
41	196535.0
42	223799.0
43	251063.0
44	251063.0
45	251063.0
46	286189.0
47	321315.0
48	321315.0
49	321315.0
50	324557.0
51	327799.0
52	327799.0
53	327799.0
54	307881.0
55	287963.0
56	287963.0
57	287963.0
58	264730.0
59	241497.0
60	241497.0
61	241497.0
62	215380.5
63	189264.0
64	189264.0
65	189264.0
66	157532.5
67	125801.0
68	125801.0
69	125801.0
70	96290.0
71	66779.0
72	66779.0
73	66779.0
74	52635.0
75	38491.0
76	38491.0
77	38491.0
78	31048.0
79	23605.0
80	23605.0
81	23605.0
82	16861.5
83	10118.0
84	10118.0
85	10118.0
86	7459.0
87	4800.0
88	4800.0
89	4800.0
90	3272.5
91	1745.0
92	1745.0
93	1745.0
94	1071.0
95	397.0
96	397.0
97	397.0
98	759.0
99	1121.0
100	1121.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009305276854046331
2	0.003168607204610604
3	0.0053745995622508985
4	0.014118351088897882
5	0.034052500211574716
6	0.04552366047130425
7	0.06597922596942335
8	0.08872100172909692
9	0.0964219205048594
10	0.12024663796737457
11	0.12004609320758908
12	0.13328204735343085
13	0.12181088709370133
14	0.12445807792286968
15	0.11423029517381013
16	0.11310724451901145
17	0.12465862268265518
18	0.13127659975557604
19	0.13360291896908763
20	0.12124936176630197
21	0.12903049844597866
22	0.150849768310639
23	0.1403011139459227
24	0.13368313687300182
25	0.1317980161310183
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2493209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.56318231057736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80506692728592	41.42245099896181
2	12.786348127010749	13.441822953731508
3	3.7067483431502293	5.845154668213082
4	1.564157074078602	3.288682937886913
5	0.8264976001858654	2.1721672018912157
6	0.5120906033603484	1.6150267046378133
7	0.3235458107738891	1.1904618206272048
8	0.23641086407393425	0.9941205878815464
9	0.16748801277921443	0.7923332655495142
>10	0.892450482056522	8.504454521313388
>50	0.08095143700904922	3.0077371420738177
>100	0.08059289111570593	9.14880586418464
>500	0.012914107286867411	4.64653142582639
>1k	0.004737719833051467	3.9302499072212624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3767	0.1510904220223816	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2579	0.10344098709735125	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2567	0.1029596796738661	No Hit
GTACATGGAAGCAGTGGTATCAACG	2552	0.10235804539450964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.010895195709626E-5
2	0.0	0.0	0.0	0.0	4.010895195709626E-5
3	0.0	0.0	0.0	0.0	4.010895195709626E-5
4	0.0	0.0	0.0	0.0	4.010895195709626E-5
5	0.0	0.0	0.0	0.0	4.010895195709626E-5
6	0.0	0.0	0.0	0.0	4.010895195709626E-5
7	0.0	0.0	0.0	0.0	4.010895195709626E-5
8	0.0	0.0	0.0	1.2032685587128877E-4	4.010895195709626E-5
9	0.0	0.0	0.0	1.6043580782838503E-4	4.010895195709626E-5
10	0.0	0.0	0.0	1.6043580782838503E-4	4.010895195709626E-5
11	0.0	0.0	0.0	1.6043580782838503E-4	4.010895195709626E-5
12	0.0	0.0	0.0	1.6043580782838503E-4	2.4065371174257754E-4
13	0.0	0.0	0.0	1.6043580782838503E-4	2.4065371174257754E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	30	7.7188056E-4	19.002089	10
CGAACGA	175	0.0	13.574011	16
AAGACGG	520	0.0	12.056354	5
CGCGTAA	145	9.640644E-11	11.7944	10
TCGCGTA	145	9.640644E-11	11.7944	9
CGCATCG	440	0.0	11.661076	13
GGTTCTA	490	0.0	11.634633	13
TCCATTA	500	0.0	11.590575	8
CGGTCCA	460	0.0	11.566489	10
CAAGACG	505	0.0	11.473743	4
CGACCAT	340	0.0	11.457142	10
CGGTTCT	515	0.0	11.43838	12
CGCAAGA	475	0.0	11.394158	2
ACGAACG	210	0.0	11.311221	15
ACGGTAT	370	0.0	11.298539	9
CGCCAGT	455	0.0	11.276871	18
GTCTTAC	155	3.1650416E-10	11.024834	1
GCTCGTA	175	2.0008883E-11	10.858336	9
TCGCCAG	495	0.0	10.749736	17
ATCGCCA	505	0.0	10.724813	16
>>END_MODULE