FastQCFastQC Report
Thu 2 Feb 2017
SRR4062478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2493209
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26930.10801340762046023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA508.3451785E-526.399884
CGCAATA7550.020.39725736
TTAACGG4500.020.04435235
CGTCGTA4850.019.50506610
TACCGTC5150.019.2232137
TAACGGC4650.018.92464436
CCGTCGT4900.018.8570589
ATACGAA8400.018.59515440
ATACCGT5450.018.5687226
CAATACG8500.018.11756538
AATACGA8750.017.5999239
CTAGCGG8800.017.4999229
GTATCAA47350.017.4261591
ACCGTCG5350.017.270958
TAGCGGC9350.016.941130
TCTAGCG9150.016.83052628
CTAACGC800.001989209616.4999263
GGCGTTA5900.016.40670442
GCAATAC9400.016.38290437
CGGTCCA9400.016.38290410
Produced by FastQC (version 0.11.5)