##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2493209
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298279446287896	32.0	32.0	32.0	32.0	32.0
2	31.407259078561005	32.0	32.0	32.0	32.0	32.0
3	31.492252354295207	32.0	32.0	32.0	32.0	32.0
4	31.59257767800453	32.0	32.0	32.0	32.0	32.0
5	31.526407533423793	32.0	32.0	32.0	32.0	32.0
6	35.11198780366989	36.0	36.0	36.0	36.0	36.0
7	35.12908584879968	36.0	36.0	36.0	36.0	36.0
8	35.07061822735278	36.0	36.0	36.0	36.0	36.0
9	35.21080823950178	36.0	36.0	36.0	36.0	36.0
10	35.07515013783441	36.0	36.0	36.0	36.0	36.0
11	35.21375103330688	36.0	36.0	36.0	36.0	36.0
12	35.11789063812942	36.0	36.0	36.0	36.0	36.0
13	35.16307216924052	36.0	36.0	36.0	36.0	36.0
14	35.12696087652499	36.0	36.0	36.0	36.0	36.0
15	35.084140559415594	36.0	36.0	36.0	36.0	36.0
16	35.1001179604277	36.0	36.0	36.0	36.0	36.0
17	35.06804082610002	36.0	36.0	36.0	36.0	36.0
18	35.08845427719858	36.0	36.0	36.0	36.0	36.0
19	35.072736381105635	36.0	36.0	36.0	36.0	36.0
20	35.06320649412063	36.0	36.0	36.0	36.0	36.0
21	35.063753580225324	36.0	36.0	36.0	36.0	36.0
22	35.0411706359154	36.0	36.0	36.0	36.0	36.0
23	35.00516763737015	36.0	36.0	36.0	36.0	36.0
24	34.98245313569781	36.0	36.0	36.0	36.0	36.0
25	34.95746365427046	36.0	36.0	36.0	32.0	36.0
26	34.91135721072722	36.0	36.0	36.0	32.0	36.0
27	34.89219114803452	36.0	36.0	36.0	32.0	36.0
28	34.88351879044236	36.0	36.0	36.0	32.0	36.0
29	34.85878039105426	36.0	36.0	36.0	32.0	36.0
30	34.84919234608891	36.0	36.0	36.0	32.0	36.0
31	34.846256370805655	36.0	36.0	36.0	32.0	36.0
32	34.81166560845882	36.0	36.0	36.0	32.0	36.0
33	34.795166389981745	36.0	36.0	36.0	32.0	36.0
34	34.787252492671094	36.0	36.0	36.0	32.0	36.0
35	34.756127945952386	36.0	36.0	36.0	32.0	36.0
36	34.732054552987734	36.0	36.0	36.0	32.0	36.0
37	34.72341829345233	36.0	36.0	36.0	32.0	36.0
38	34.68870158899635	36.0	36.0	36.0	32.0	36.0
39	34.6819251815632	36.0	36.0	36.0	32.0	36.0
40	34.672932754534415	36.0	36.0	36.0	32.0	36.0
41	34.6460790090201	36.0	36.0	36.0	32.0	36.0
42	34.62097922797487	36.0	36.0	36.0	32.0	36.0
43	34.61447395705695	36.0	36.0	36.0	32.0	36.0
44	34.58898913007293	36.0	36.0	36.0	32.0	36.0
45	34.547485188766764	36.0	36.0	36.0	32.0	36.0
46	34.55774906957259	36.0	36.0	36.0	32.0	36.0
47	34.53128197435514	36.0	36.0	36.0	32.0	36.0
48	34.49625843641668	36.0	36.0	36.0	32.0	36.0
49	34.48714889124819	36.0	36.0	36.0	32.0	36.0
50	33.97561656483672	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	9.0
21	58.0
22	174.0
23	536.0
24	1524.0
25	3621.0
26	7423.0
27	13973.0
28	23635.0
29	37025.0
30	54521.0
31	79084.0
32	118005.0
33	195383.0
34	432902.0
35	1525330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.200591371559206	17.744556529496975	11.31360142551755	26.74125067342627
2	16.525491712405803	20.27660878480172	36.19001702281957	27.007882479972917
3	18.6924884305032	23.655479677875046	28.42650832626743	29.225523565354322
4	12.55869956890812	15.47394437037643	35.8320057243611	36.13535033635434
5	14.551974805150634	36.426844451004484	33.48637578573469	15.534804958110193
6	34.835699167455815	35.16134479329986	16.417288457689533	13.585667581554798
7	30.536694305767632	30.24113120170126	20.61020204098577	18.611972451545338
8	28.367056271656327	32.70495975267216	19.35084463436479	19.577139341306726
9	27.33906383299595	14.156895791728651	18.436641292406694	40.067399082868704
10	15.901996182429952	26.53098877791633	31.56273701883797	26.004278020815747
11	37.82562954008268	21.014924942112756	21.950987662887467	19.208457854917096
12	24.735511543556918	23.595334366272542	28.416029301995945	23.2531247881746
13	29.454971484540604	19.17235979815571	25.294710551742757	26.077958165560926
14	23.36799682657972	19.594025210080662	24.792907453807523	32.24507050953209
15	25.32900370566607	26.964927529140155	22.105126365258588	25.600942399935185
16	26.017072776490057	25.632267491413675	23.48126450690656	24.869395225189706
17	24.23892260937611	25.84488504573824	25.020365320356213	24.89582702452943
18	25.18769986792122	24.650320129600047	26.01165806797585	24.150321934502884
19	25.660945392063	25.085823129950196	25.08542204043063	24.16780943755618
20	25.646947367829974	24.343125666560645	24.937780988276554	25.072145977332827
21	26.61152755344618	24.26916475915176	24.242091216580718	24.877216470821338
22	25.668405657127018	24.532600355605968	24.724321145960886	25.07467284130612
23	24.52373627722345	24.22456360457547	25.358804656970197	25.892895461230886
24	24.834620763842903	25.08987413409786	24.94018752539398	25.135317576665255
25	24.958477207486414	24.57511584468049	25.128619381688416	25.337787566144677
26	24.57086429577304	25.203743448704056	25.534963173965757	24.69042908155714
27	25.401721235564285	24.883593794182516	24.65697821562492	25.057706754628274
28	24.603753636377856	24.637084175454206	25.48446600345178	25.274696184716163
29	24.646830650779776	24.90348783435324	25.379942074651584	25.0697394402154
30	24.687741781776015	24.91463812299731	25.624366027878125	24.773254067348546
31	25.110048936932284	24.73466925556582	24.6777546527387	25.477527154763198
32	24.776583110360985	24.866186509033135	24.74473660250705	25.612493778098827
33	24.476848912385606	24.439146497545934	25.337988110904462	25.746016479164002
34	25.054778801135402	24.60222949620349	25.5739089663161	24.76908273634501
35	25.63728111040831	24.528027935082857	25.25941467402051	24.575276280488318
36	24.59833892786365	24.984267263594827	25.140812503083374	25.276581305458144
37	25.63487457329089	24.711967588758103	24.730899014081853	24.922258823869157
38	24.706272117580195	24.569380264550624	25.387843538187134	25.336504079682047
39	25.157658262905358	24.57150603900435	24.701860132864915	25.568975565225376
40	25.397309250849005	24.689586793566043	25.359606836009334	24.553497119575614
41	24.54375064425004	24.924665360986584	25.620836440105904	24.910747554657473
42	25.56769207876275	25.116426260293462	25.049283874717283	24.266597786226505
43	24.701980459720787	24.25380303055219	25.44311367398401	25.60110283574301
44	24.566612746865584	24.63203044750761	25.251192338869306	25.5501644667575
45	24.53396405997251	24.98575129481724	25.305299315059425	25.17498533015082
46	24.50835409975987	24.498727943568262	25.505784317146553	25.487133639525318
47	24.704828195309737	24.405535195805886	25.71056016563393	25.179076443250448
48	25.246499591490323	25.256045522056112	24.80341599922028	24.69403888723328
49	24.276143716792294	25.01727693105552	25.295592948685808	25.410986403466374
50	24.129956265181242	25.466547516292266	25.12919098607096	25.274305232455536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	31.0
2	36.0
3	123.5
4	211.0
5	257.5
6	304.0
7	291.0
8	278.0
9	370.5
10	463.0
11	687.0
12	911.0
13	1596.5
14	2282.0
15	3165.0
16	4048.0
17	4863.0
18	5678.0
19	6276.5
20	6875.0
21	8491.0
22	10107.0
23	11003.5
24	11900.0
25	14945.0
26	17990.0
27	22363.5
28	26737.0
29	30608.5
30	34480.0
31	37147.5
32	39815.0
33	45890.5
34	51966.0
35	60212.0
36	68458.0
37	82011.0
38	95564.0
39	102087.5
40	108611.0
41	118858.0
42	129105.0
43	131434.0
44	133763.0
45	147028.5
46	160294.0
47	169809.0
48	179324.0
49	183873.5
50	188423.0
51	182102.5
52	175782.0
53	173005.5
54	170229.0
55	171621.0
56	173013.0
57	169923.5
58	166834.0
59	152264.5
60	137695.0
61	122079.0
62	106463.0
63	93283.5
64	80104.0
65	68783.5
66	57463.0
67	50991.5
68	44520.0
69	40911.0
70	37302.0
71	29509.0
72	21716.0
73	19309.5
74	16903.0
75	12590.0
76	8277.0
77	7488.5
78	6700.0
79	5827.0
80	4954.0
81	3979.5
82	3005.0
83	2578.0
84	2151.0
85	1785.5
86	1420.0
87	1062.5
88	705.0
89	471.0
90	237.0
91	143.5
92	50.0
93	33.5
94	17.0
95	11.5
96	6.0
97	7.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014439222704554652
2	0.0034092609163531816
3	6.016342793564438E-4
4	2.005447597854813E-4
5	4.010895195709626E-5
6	3.2087161565677006E-4
7	8.021790391419251E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.021790391419251E-5
47	0.0
48	0.0
49	0.0
50	4.010895195709626E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2493209.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.72101882827447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7196196390285	43.07617788421882
2	13.037930503160819	14.268976810903943
3	3.755484358716571	6.165117909078593
4	1.5388895620433196	3.368384187968302
5	0.7985477652708313	2.184867364933083
6	0.47536909529052485	1.5607608728263567
7	0.30767475850268383	1.1785393377107112
8	0.22455125417708718	0.9830138726189628
9	0.15286106605687536	0.7528241946427545
>10	0.8279095257481235	8.071783603130969
>50	0.07523346521547267	2.877760284033206
>100	0.07119983137543488	8.367892995107086
>500	0.010973235683331362	4.095948156195117
>1k	0.0037559397305354067	3.0479525266320007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2693	0.10801340762046023	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.021790391419251E-5
2	0.0	0.0	0.0	0.0	8.021790391419251E-5
3	0.0	0.0	0.0	0.0	8.021790391419251E-5
4	0.0	0.0	0.0	0.0	8.021790391419251E-5
5	0.0	0.0	0.0	0.0	8.021790391419251E-5
6	0.0	0.0	0.0	0.0	8.021790391419251E-5
7	0.0	0.0	0.0	0.0	8.021790391419251E-5
8	0.0	0.0	0.0	0.0	8.021790391419251E-5
9	0.0	0.0	0.0	0.0	8.021790391419251E-5
10	0.0	0.0	0.0	4.010895195709626E-5	1.2032685587128877E-4
11	0.0	0.0	0.0	4.010895195709626E-5	1.2032685587128877E-4
12	0.0	0.0	0.0	4.010895195709626E-5	2.0054475978548128E-4
13	0.0	0.0	0.0	4.010895195709626E-5	2.4065371174257754E-4
14	0.0	0.0	0.0	8.021790391419251E-5	2.4065371174257754E-4
15	0.0	0.0	0.0	1.2032685587128877E-4	2.8076266369967377E-4
16	0.0	0.0	0.0	1.6043580782838503E-4	2.8076266369967377E-4
17	0.0	0.0	0.0	2.0054475978548128E-4	2.8076266369967377E-4
18	0.0	0.0	0.0	2.4065371174257754E-4	3.2087161565677006E-4
19	0.0	0.0	0.0	2.8076266369967377E-4	3.2087161565677006E-4
20	0.0	0.0	0.0	5.615253273993475E-4	3.609805676138663E-4
21	0.0	0.0	0.0	7.620700871848289E-4	4.0108951957096257E-4
22	0.0	0.0	0.0	0.0014439222704554651	4.813074234851551E-4
23	0.0	0.0	0.0	0.0021257744537261014	4.813074234851551E-4
24	0.0	0.0	0.0	0.003288934060481893	4.813074234851551E-4
25	0.0	0.0	0.0	0.004051004147666722	4.813074234851551E-4
26	0.0	0.0	0.0	0.005214163754422513	6.417432313135401E-4
27	0.0	0.0	0.0	0.0073800471601057115	6.818521832706363E-4
28	0.0	0.0	0.0	0.01371726156932692	6.818521832706363E-4
29	0.0	0.0	0.0	0.026110927724069664	6.818521832706363E-4
30	0.0	0.0	0.0	0.049334010907228394	6.818521832706363E-4
31	0.0	0.0	0.0	0.09578017727354586	7.620700871848289E-4
32	0.0	0.0	0.0	0.15125085783020997	7.620700871848289E-4
33	0.0	0.0	0.0	0.20900774864842858	7.620700871848289E-4
34	0.0	0.0	0.0	0.2761501342246077	8.021790391419251E-4
35	0.0	0.0	0.0	0.34746385080432485	8.021790391419251E-4
36	0.0	0.0	0.0	0.4520679975084319	8.021790391419251E-4
37	0.0	0.0	0.0	0.602917765819071	8.021790391419251E-4
38	0.0	0.0	0.0	0.7992911946010142	8.422879910990214E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	50	8.3451785E-5	26.39988	4
CGCAATA	755	0.0	20.397257	36
TTAACGG	450	0.0	20.044352	35
CGTCGTA	485	0.0	19.505066	10
TACCGTC	515	0.0	19.223213	7
TAACGGC	465	0.0	18.924644	36
CCGTCGT	490	0.0	18.857058	9
ATACGAA	840	0.0	18.595154	40
ATACCGT	545	0.0	18.568722	6
CAATACG	850	0.0	18.117565	38
AATACGA	875	0.0	17.59992	39
CTAGCGG	880	0.0	17.49992	29
GTATCAA	4735	0.0	17.426159	1
ACCGTCG	535	0.0	17.27095	8
TAGCGGC	935	0.0	16.9411	30
TCTAGCG	915	0.0	16.830526	28
CTAACGC	80	0.0019892096	16.499926	3
GGCGTTA	590	0.0	16.406704	42
GCAATAC	940	0.0	16.382904	37
CGGTCCA	940	0.0	16.382904	10
>>END_MODULE