FastQCFastQC Report
Thu 2 Feb 2017
SRR4062477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301327
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG86602.873954209214574No Hit
TCCATGTACTCTGCGTTGATACCAC77502.5719567114795554No Hit
GAGTACATGGAAGCAGTGGTATCAA40161.3327713746195993No Hit
CATGTACTCTGCGTTGATACCACTG35771.1870824718661122No Hit
GCTTCCATGTACTCTGCGTTGATAC26350.8744652819030487No Hit
CCCATGTACTCTGCGTTGATACCAC24200.8031142247458741No Hit
GCGTTGATACCACTGCTTCCATGTA21380.7095281869862309No Hit
CATGGAAGCAGTGGTATCAACGCAG19680.6531110720247438No Hit
GTACATGGGAAGCAGTGGTATCAAC19670.6527792066426175No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19200.6371815336826769No Hit
GTATCAACGCAGAGTACTTTTTTTT17360.5761183033714203No Hit
GTATCAACGCAGAGTACATGGAAGC17330.5751227072250412No Hit
ACTCTGCGTTGATACCACTGCTTCC14570.48352786175815643No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14330.475563092587123No Hit
ACGCAGAGTACATGGAAGCAGTGGT14270.4735719002943646No Hit
TATCAACGCAGAGTACTTTTTTTTT13020.43208872752856536No Hit
CAGTGGTATCAACGCAGAGTACATG13000.43142499676431256No Hit
TATCAACGCAGAGTACATGGAAGCA11920.39558353549466196No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9170.3043205554099035No Hit
GTATCAACGCAGAGTACATGGGAAG9170.3043205554099035No Hit
GTACATGGGGTGGTATCAACGCAAA9110.3023293631171452No Hit
ATACCACTGCTTCCATGTACTCTGC8500.2820855748074351No Hit
GGTATCAACGCAGAGTACATGGAAG7650.2538770173266916No Hit
GAGTACATGGGAAGCAGTGGTATCA6910.22931897904933846No Hit
AAGCAGTGGTATCAACGCAGAGTAC6830.22666405599232725No Hit
GCGTTGATACCACTGCTTCCCATGT6530.21670809452853543No Hit
GCTTCCCATGTACTCTGCGTTGATA6130.20343347924347965No Hit
GGTATCAACGCAGAGTACTTTTTTT5960.19779176774733098No Hit
CCACTGCTTCCATGTACTCTGCGTT5780.19181819086905588No Hit
TATCAACGCAGAGTACATGGGAAGC5740.1904907293405503No Hit
CTGCTTCCATGTACTCTGCGTTGAT5550.1841852870801488No Hit
GTACTCTGCGTTGATACCACTGCTT5520.18318969093376963No Hit
AAAAAGTACTCTGCGTTGATACCAC5430.18020290249463208No Hit
GTACATGGGTGGTATCAACGCAAAA5420.17987103711250568No Hit
GTGGTATCAACGCAGAGTACATGGA5410.17953917173037928No Hit
ACATGGAAGCAGTGGTATCAACGCA5210.1729018640878514No Hit
GAAGCAGTGGTATCAACGCAGAGTA5170.17157440255934583No Hit
GTCCTAAAGTGTGTATTTCTCATTT5030.1669282872095763No Hit
GTCCTACAGTGGACATTTCTAAATT4890.16228217185980678No Hit
ACGCAGAGTACATGGGAAGCAGTGG4820.15995911418492204No Hit
GGTATCAACGCAGAGTACATGGGAA4480.1486756911926246No Hit
GATACCACTGCTTCCATGTACTCTG4480.1486756911926246No Hit
GTTGATACCACTGCTTCCATGTACT4470.14834382581049824No Hit
GTACATGGGAGTGGTATCAACGCAA4350.14436144122498148No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4240.14071092202159116No Hit
TGGAAGCAGTGGTATCAACGCAGAG4160.13805599896458No Hit
CTGTAGGACGTGGAATATGGCAAGA4030.1337417489969369No Hit
CTTTAGGACGTGAAATATGGCGAGG3990.13241428746843129No Hit
TTGTAGAACAGTGTATATCAATGAG3890.12909563364716736No Hit
CCCCATGTACTCTGCGTTGATACCA3780.12544511444377704No Hit
GTCCTACAGTGTGCATTTCTCATTT3680.12212646062251307No Hit
GTATTAGAGGCACTGCCTGCCCAGT3640.1207989990940075No Hit
CCATAGGGTCTTCTCGTCTTATTAT3540.11748034527274356No Hit
GCAGAGTACATGGAAGCAGTGGTAT3520.11681661450849079No Hit
GTGTATATCAATGAGTTACAATGAG3520.11681661450849079No Hit
GCAGTGGTATCAACGCAGAGTACAT3430.11382982606935324No Hit
CTGTAGGACCTGGAATATGGCGAGA3370.11183863377659485No Hit
GATATACACTGTTCTACAAATCCCG3320.1101793068659629No Hit
CTTCCATGTACTCTGCGTTGATACC3200.10619692228044615No Hit
GTATCAACGCAGAGTACATGGGGTG3190.10586505689831978No Hit
GTGTATATCAATGAGTTACAATGAA3040.10088707616642384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGTG250.00601585519.00282515
GCAGAAG250.0060274218.996513
GGAGCTT402.7522095E-416.624718
GAGCTTA402.7522095E-416.624719
TCCACTG350.00216258416.28813612
TTGTAGA851.0113581E-915.6415861
TCGTCTT456.7323336E-414.77997614
ACGGACC400.00526082414.25211916
TCCAACG400.00526082414.25211918
CGGACCA400.00526082414.25211917
CTCGTCT551.9494336E-413.82023813
AGCTTAA551.9521858E-413.8179410
TAGAAAT707.217406E-613.5711914
CGTCTTA500.001492976413.30197715
TCTCGTC500.001492976413.30197712
TAGAGTG500.001494848513.29976755
TAACTTT1009.951691E-913.29976756
ACCCCAT655.42493E-513.1536166
ATACCAA655.42493E-513.15361619
ACTTTAT957.311064E-812.9997728
Produced by FastQC (version 0.11.5)