##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062477_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301327
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.806651909719342	32.0	32.0	32.0	32.0	32.0
2	30.71412452252868	32.0	32.0	32.0	32.0	32.0
3	30.743355225386374	32.0	32.0	32.0	32.0	32.0
4	30.680772051624977	32.0	32.0	32.0	32.0	32.0
5	30.681628264310866	32.0	32.0	32.0	32.0	32.0
6	34.19416116046687	36.0	36.0	36.0	32.0	36.0
7	33.983134601280334	36.0	36.0	36.0	32.0	36.0
8	33.95807212762215	36.0	36.0	36.0	32.0	36.0
9	34.009992466655824	36.0	36.0	36.0	32.0	36.0
10	33.87123955038878	36.0	36.0	36.0	32.0	36.0
11	34.169188290461854	36.0	36.0	36.0	32.0	36.0
12	34.00803114224746	36.0	36.0	36.0	32.0	36.0
13	34.056440345538235	36.0	36.0	36.0	32.0	36.0
14	33.986068291258334	36.0	36.0	36.0	32.0	36.0
15	34.009567678966704	36.0	36.0	36.0	32.0	36.0
16	33.952855203815126	36.0	36.0	36.0	32.0	36.0
17	33.922472928081454	36.0	36.0	36.0	32.0	36.0
18	33.96604353410083	36.0	36.0	36.0	32.0	36.0
19	33.925433167290024	36.0	36.0	36.0	32.0	36.0
20	33.86532902793311	36.0	36.0	36.0	32.0	36.0
21	33.729211122800145	36.0	36.0	36.0	32.0	36.0
22	33.72377516784092	36.0	36.0	36.0	27.0	36.0
23	33.6168016805663	36.0	36.0	36.0	27.0	36.0
24	33.655928609118995	36.0	36.0	36.0	27.0	36.0
25	33.048249907907355	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	8.0
5	29.0
6	53.0
7	17.0
8	60.0
9	67.0
10	35.0
11	11.0
12	27.0
13	21.0
14	77.0
15	107.0
16	158.0
17	211.0
18	308.0
19	459.0
20	684.0
21	1055.0
22	1525.0
23	2027.0
24	2576.0
25	3159.0
26	4055.0
27	4962.0
28	6736.0
29	8481.0
30	10815.0
31	14532.0
32	20782.0
33	29632.0
34	64967.0
35	123689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.74048628950356	18.202335198571536	12.966724416034412	23.090454095890504
2	11.595838242370942	23.27497801304283	40.33586114199426	24.79332260259197
3	19.916166909492787	28.879935482372147	28.80327101718147	22.400626590953593
4	11.08278817207513	18.440556678515836	37.31757427303535	33.15908087637369
5	10.511201412958314	40.46253137325206	36.05135253575555	12.974914678034075
6	29.667863107669728	35.953660877043276	20.30507585391688	14.07340016137012
7	24.9041051306661	33.393997562360724	23.6557282862466	18.046169020726577
8	26.87545883673883	34.75765115452459	23.142313301199536	15.224576707537047
9	26.8729257540372	16.6016085684007	19.89914056867976	36.62632510888234
10	14.509965507845472	29.66724042826097	35.66050149864756	20.162292565246
11	33.1638620836324	23.67114602440386	22.417723369753038	20.747268522210703
12	20.994247468520594	26.99768370049616	32.71066860300886	19.297400227974386
13	31.146533219025592	22.35181529995813	26.167833004366294	20.333818476649988
14	22.825602956129913	21.58945150763955	27.30299667036626	28.281948865864276
15	24.76516422721005	28.292934159591965	28.64946586699008	18.292435746207904
16	23.011593018271714	27.280973693916405	30.24717816830976	19.460255119502122
17	20.47458911256289	27.518326277522647	31.96813919331149	20.038945416602978
18	22.91129396751123	24.712200568952227	34.58013186930051	17.796373594236034
19	21.285031122159044	32.28263506485306	27.401457573834126	19.03087623915377
20	20.461569135679394	25.677400059814577	35.09819559365965	18.762835210846376
21	21.220741843608653	29.427149973911508	25.388253201240286	23.963854981239553
22	19.407142477264266	31.148619901079616	27.59931925756528	21.844918364090837
23	19.310023929805904	29.82517947354427	27.106155277851634	23.758641318798194
24	19.31562119872643	32.438199185073415	27.814853466096796	20.431326150103363
25	23.380115054453128	27.75065553122123	26.333245818392097	22.535983595933544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	92.0
1	92.0
2	290.0
3	488.0
4	488.0
5	488.0
6	1574.5
7	2661.0
8	2661.0
9	2661.0
10	2590.0
11	2519.0
12	2519.0
13	2519.0
14	2079.5
15	1640.0
16	1640.0
17	1640.0
18	2638.0
19	3636.0
20	3636.0
21	3636.0
22	5768.5
23	7901.0
24	7901.0
25	7901.0
26	11695.5
27	15490.0
28	15490.0
29	15490.0
30	19451.0
31	23412.0
32	23412.0
33	23412.0
34	24823.5
35	26235.0
36	26235.0
37	26235.0
38	26601.5
39	26968.0
40	26968.0
41	26968.0
42	31318.0
43	35668.0
44	35668.0
45	35668.0
46	54747.0
47	73826.0
48	73826.0
49	73826.0
50	56145.5
51	38465.0
52	38465.0
53	38465.0
54	27887.0
55	17309.0
56	17309.0
57	17309.0
58	14022.5
59	10736.0
60	10736.0
61	10736.0
62	8631.5
63	6527.0
64	6527.0
65	6527.0
66	5213.0
67	3899.0
68	3899.0
69	3899.0
70	2976.0
71	2053.0
72	2053.0
73	2053.0
74	1548.5
75	1044.0
76	1044.0
77	1044.0
78	701.5
79	359.0
80	359.0
81	359.0
82	237.5
83	116.0
84	116.0
85	116.0
86	92.0
87	68.0
88	68.0
89	68.0
90	44.5
91	21.0
92	21.0
93	21.0
94	17.0
95	13.0
96	13.0
97	13.0
98	97.0
99	181.0
100	181.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008296634553159857
2	0.003982384585516731
3	0.004646115349769519
4	0.013274615285055769
5	0.03816451894453534
6	0.05177099961171751
7	0.07168292253930116
8	0.09756842234515992
9	0.1042057299876878
10	0.12909563364716736
11	0.12975936441142016
12	0.13805599896458
13	0.1277681721186618
14	0.13042309517567294
15	0.12345392215101866
16	0.12279019138676586
17	0.13042309517567294
18	0.14037905663946476
19	0.13805599896458
20	0.1317505567041785
21	0.14203838355009674
22	0.15896351803854283
23	0.14834382581049824
24	0.14635263351773986
25	0.14137465278584394
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	301327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.29519933162068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98734984905497	33.407857065422384
2	12.615500943981282	10.671502541878743
3	3.6042353162619576	4.573255534180992
4	1.457899607817975	2.4664861807261147
5	0.6962068784930879	1.4723104350955287
6	0.4474999054203208	1.1356258620380322
7	0.30061883722137694	0.8900313550182719
8	0.21112684693077266	0.7143721660154878
9	0.15147524151344482	0.5766007980254881
>10	1.2478981736203274	10.493944635736607
>50	0.1389169376586815	4.112531747910456
>100	0.1114474867092247	9.019752413772128
>500	0.01569682911397531	4.322656261303572
>1k	0.01255746329118025	10.695769267045184
>5k	0.0015696829113975312	5.447303735831025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8660	2.873954209214574	No Hit
TCCATGTACTCTGCGTTGATACCAC	7750	2.5719567114795554	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4016	1.3327713746195993	No Hit
CATGTACTCTGCGTTGATACCACTG	3577	1.1870824718661122	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2635	0.8744652819030487	No Hit
CCCATGTACTCTGCGTTGATACCAC	2420	0.8031142247458741	No Hit
GCGTTGATACCACTGCTTCCATGTA	2138	0.7095281869862309	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1968	0.6531110720247438	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1967	0.6527792066426175	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1920	0.6371815336826769	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1736	0.5761183033714203	No Hit
GTATCAACGCAGAGTACATGGAAGC	1733	0.5751227072250412	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1457	0.48352786175815643	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1433	0.475563092587123	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1427	0.4735719002943646	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1302	0.43208872752856536	No Hit
CAGTGGTATCAACGCAGAGTACATG	1300	0.43142499676431256	No Hit
TATCAACGCAGAGTACATGGAAGCA	1192	0.39558353549466196	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	917	0.3043205554099035	No Hit
GTATCAACGCAGAGTACATGGGAAG	917	0.3043205554099035	No Hit
GTACATGGGGTGGTATCAACGCAAA	911	0.3023293631171452	No Hit
ATACCACTGCTTCCATGTACTCTGC	850	0.2820855748074351	No Hit
GGTATCAACGCAGAGTACATGGAAG	765	0.2538770173266916	No Hit
GAGTACATGGGAAGCAGTGGTATCA	691	0.22931897904933846	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	683	0.22666405599232725	No Hit
GCGTTGATACCACTGCTTCCCATGT	653	0.21670809452853543	No Hit
GCTTCCCATGTACTCTGCGTTGATA	613	0.20343347924347965	No Hit
GGTATCAACGCAGAGTACTTTTTTT	596	0.19779176774733098	No Hit
CCACTGCTTCCATGTACTCTGCGTT	578	0.19181819086905588	No Hit
TATCAACGCAGAGTACATGGGAAGC	574	0.1904907293405503	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	555	0.1841852870801488	No Hit
GTACTCTGCGTTGATACCACTGCTT	552	0.18318969093376963	No Hit
AAAAAGTACTCTGCGTTGATACCAC	543	0.18020290249463208	No Hit
GTACATGGGTGGTATCAACGCAAAA	542	0.17987103711250568	No Hit
GTGGTATCAACGCAGAGTACATGGA	541	0.17953917173037928	No Hit
ACATGGAAGCAGTGGTATCAACGCA	521	0.1729018640878514	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	517	0.17157440255934583	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	503	0.1669282872095763	No Hit
GTCCTACAGTGGACATTTCTAAATT	489	0.16228217185980678	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	482	0.15995911418492204	No Hit
GGTATCAACGCAGAGTACATGGGAA	448	0.1486756911926246	No Hit
GATACCACTGCTTCCATGTACTCTG	448	0.1486756911926246	No Hit
GTTGATACCACTGCTTCCATGTACT	447	0.14834382581049824	No Hit
GTACATGGGAGTGGTATCAACGCAA	435	0.14436144122498148	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	424	0.14071092202159116	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	416	0.13805599896458	No Hit
CTGTAGGACGTGGAATATGGCAAGA	403	0.1337417489969369	No Hit
CTTTAGGACGTGAAATATGGCGAGG	399	0.13241428746843129	No Hit
TTGTAGAACAGTGTATATCAATGAG	389	0.12909563364716736	No Hit
CCCCATGTACTCTGCGTTGATACCA	378	0.12544511444377704	No Hit
GTCCTACAGTGTGCATTTCTCATTT	368	0.12212646062251307	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	364	0.1207989990940075	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	354	0.11748034527274356	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	352	0.11681661450849079	No Hit
GTGTATATCAATGAGTTACAATGAG	352	0.11681661450849079	No Hit
GCAGTGGTATCAACGCAGAGTACAT	343	0.11382982606935324	No Hit
CTGTAGGACCTGGAATATGGCGAGA	337	0.11183863377659485	No Hit
GATATACACTGTTCTACAAATCCCG	332	0.1101793068659629	No Hit
CTTCCATGTACTCTGCGTTGATACC	320	0.10619692228044615	No Hit
GTATCAACGCAGAGTACATGGGGTG	319	0.10586505689831978	No Hit
GTGTATATCAATGAGTTACAATGAA	304	0.10088707616642384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.3186538212639426E-4	0.0
9	0.0	0.0	0.0	3.3186538212639426E-4	0.0
10	0.0	0.0	0.0	3.3186538212639426E-4	0.0
11	0.0	0.0	0.0	3.3186538212639426E-4	0.0
12	0.0	0.0	0.0	3.3186538212639426E-4	0.0
13	0.0	0.0	0.0	3.3186538212639426E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGGTG	25	0.006015855	19.002825	15
GCAGAAG	25	0.00602742	18.99651	3
GGAGCTT	40	2.7522095E-4	16.62471	8
GAGCTTA	40	2.7522095E-4	16.62471	9
TCCACTG	35	0.002162584	16.288136	12
TTGTAGA	85	1.0113581E-9	15.641586	1
TCGTCTT	45	6.7323336E-4	14.779976	14
ACGGACC	40	0.005260824	14.252119	16
TCCAACG	40	0.005260824	14.252119	18
CGGACCA	40	0.005260824	14.252119	17
CTCGTCT	55	1.9494336E-4	13.820238	13
AGCTTAA	55	1.9521858E-4	13.81794	10
TAGAAAT	70	7.217406E-6	13.571191	4
CGTCTTA	50	0.0014929764	13.301977	15
TCTCGTC	50	0.0014929764	13.301977	12
TAGAGTG	50	0.0014948485	13.2997675	5
TAACTTT	100	9.951691E-9	13.2997675	6
ACCCCAT	65	5.42493E-5	13.153616	6
ATACCAA	65	5.42493E-5	13.153616	19
ACTTTAT	95	7.311064E-8	12.999772	8
>>END_MODULE