##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301327
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03773309394777	32.0	32.0	32.0	32.0	32.0
2	31.419876081466313	32.0	32.0	32.0	32.0	32.0
3	31.515735397093522	32.0	32.0	32.0	32.0	32.0
4	31.637105204644786	32.0	32.0	32.0	32.0	32.0
5	31.587265661557048	32.0	32.0	32.0	32.0	32.0
6	35.1496082329164	36.0	36.0	36.0	36.0	36.0
7	35.166108579715726	36.0	36.0	36.0	36.0	36.0
8	35.13483358610413	36.0	36.0	36.0	36.0	36.0
9	35.18314986708791	36.0	36.0	36.0	36.0	36.0
10	35.14236361162458	36.0	36.0	36.0	36.0	36.0
11	35.2050131584624	36.0	36.0	36.0	36.0	36.0
12	35.13464774149014	36.0	36.0	36.0	36.0	36.0
13	35.15106512194394	36.0	36.0	36.0	36.0	36.0
14	35.11917285872159	36.0	36.0	36.0	36.0	36.0
15	35.12335436253638	36.0	36.0	36.0	36.0	36.0
16	35.10016692828721	36.0	36.0	36.0	36.0	36.0
17	35.10297450942	36.0	36.0	36.0	36.0	36.0
18	35.10851997995533	36.0	36.0	36.0	36.0	36.0
19	35.03811805779104	36.0	36.0	36.0	36.0	36.0
20	34.99210160390539	36.0	36.0	36.0	36.0	36.0
21	34.89735071865449	36.0	36.0	36.0	32.0	36.0
22	34.87972534820975	36.0	36.0	36.0	32.0	36.0
23	34.788880518506474	36.0	36.0	36.0	32.0	36.0
24	34.79458196577141	36.0	36.0	36.0	32.0	36.0
25	34.77780948935874	36.0	36.0	36.0	32.0	36.0
26	34.71096184543702	36.0	36.0	36.0	32.0	36.0
27	34.67751645222632	36.0	36.0	36.0	32.0	36.0
28	34.58511849253469	36.0	36.0	36.0	32.0	36.0
29	34.5314326296681	36.0	36.0	36.0	32.0	36.0
30	34.487935034032795	36.0	36.0	36.0	32.0	36.0
31	34.43863643151792	36.0	36.0	36.0	32.0	36.0
32	34.37397909911823	36.0	36.0	36.0	32.0	36.0
33	34.26606975146601	36.0	36.0	36.0	32.0	36.0
34	34.212108440332266	36.0	36.0	36.0	32.0	36.0
35	34.03346198647981	36.0	36.0	36.0	32.0	36.0
36	33.96630902640653	36.0	36.0	36.0	32.0	36.0
37	33.830214351850316	36.0	36.0	36.0	32.0	36.0
38	33.678601652025876	36.0	36.0	36.0	27.0	36.0
39	33.37441716142264	36.0	36.0	36.0	21.0	36.0
40	33.15151977751745	36.0	36.0	36.0	14.0	36.0
41	33.1942441268124	36.0	36.0	36.0	14.0	36.0
42	32.87296856902966	36.0	36.0	36.0	14.0	36.0
43	32.89831312826265	36.0	36.0	36.0	14.0	36.0
44	32.92372406057207	36.0	36.0	36.0	14.0	36.0
45	32.470306344934244	36.0	36.0	36.0	14.0	36.0
46	32.6693824317104	36.0	36.0	36.0	14.0	36.0
47	32.49783457838163	36.0	36.0	36.0	14.0	36.0
48	32.46822886764213	36.0	36.0	36.0	14.0	36.0
49	32.56206380443837	36.0	36.0	36.0	14.0	36.0
50	31.815618912344398	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	20.0
22	37.0
23	103.0
24	354.0
25	869.0
26	1765.0
27	3622.0
28	6053.0
29	8962.0
30	12411.0
31	17088.0
32	24480.0
33	29833.0
34	47094.0
35	148634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.542397859776884	18.744544057462072	13.397305469717239	23.31575261304381
2	11.370786815170785	23.833450596715743	39.961701336802555	24.834061251310917
3	19.501405449893305	29.212782127057977	29.1829142426666	22.10289818038211
4	11.12748319096261	18.58717800654441	37.2307733152798	33.05456548721318
5	10.454755133127797	40.62297769532767	35.89920584614057	13.023061325403962
6	29.817405848853724	36.07512179580784	20.074736828131844	14.032735527206594
7	24.97187440886479	33.41452973016026	23.547840054160428	18.065755806814522
8	26.858529106253336	34.84354206559651	23.015859846611818	15.28206898153833
9	26.810076760462888	16.226557859070045	20.067899657183062	36.895465723284005
10	14.581833025251637	29.96312975604576	35.39012434995868	20.064912868743924
11	33.18687007802155	23.697511341499432	22.457662273875226	20.65795630660379
12	20.789043132543714	27.084529431481414	32.71827615845908	19.40815127751579
13	31.350327053334087	22.26849900606318	26.326217033322603	20.05495690728013
14	23.04639146176745	21.57921460738666	27.271037776236444	28.103356154609443
15	24.767777198857054	28.23742976898851	28.898505610184284	18.096287421970153
16	23.238873383400758	27.083533835335032	30.223312215632852	19.454280565631358
17	20.685832998702406	27.495046909171762	31.659957454858013	20.15916263726782
18	22.906676135892237	24.339007125149752	34.741991258665834	18.012325480292173
19	21.423901608551507	31.99381402927716	27.84217809887597	18.740106263295356
20	20.685169267938154	25.266902733575154	35.4833785223362	18.564549476150493
21	21.326665051588474	28.690757880973162	25.807843306441175	24.17473376099719
22	19.380938316181425	30.9079504989596	27.868395464063955	21.842715720795017
23	19.298967566796204	29.417543067829964	27.492060120732624	23.791429244641204
24	19.345096854911773	32.03994331739273	28.035655616655657	20.579304211039833
25	23.70348491837771	26.95510193245212	26.658082415448998	22.683330733721174
26	17.834445635472427	27.292609026074665	30.513362559611316	24.35958277884159
27	22.88709607834678	29.89609294885622	29.364444606689744	17.852366366107255
28	20.56768892266541	26.24391441855526	29.07505799347553	24.113338665303807
29	19.112791087423297	29.528054240078056	31.32311409199972	20.036040580498927
30	23.826606975146603	26.61261685809768	30.152956754622057	19.407819412133662
31	19.087901183763815	27.697152926886737	29.692991334994872	23.521954554354572
32	18.55758030312584	29.972753852127422	30.518340540343214	20.95132530440352
33	19.11179549127692	28.81985351462033	30.104504408831602	21.963846585271153
34	17.42094136934294	31.031736286492745	32.588184928665534	18.959137415498777
35	21.83773774006312	29.51776641323214	29.2771640111905	19.367331835514243
36	20.323104136038257	30.0567157938054	29.979723025152076	19.640457045004265
37	22.125465026366705	29.251942242148893	29.336236049208996	19.2863566822754
38	20.926103535361914	30.546880963206085	26.328208225615363	22.198807275816637
39	19.696542294583626	29.105589608631156	30.19974977350188	20.998118323283343
40	23.03046192342538	29.155037550567986	26.878109163798797	20.936391362207836
41	19.053387184022675	28.59053453557099	30.396877810484956	21.95920046992138
42	23.780145821648908	30.318557580303125	28.038642405094798	17.86265419295317
43	21.27721710965164	27.58564615849227	27.52093240897762	23.616204322878467
44	20.591251364796385	28.354578248879125	31.99879201000906	19.05537837631543
45	22.862538040069428	28.324046633723498	28.709342342372242	20.104072983834836
46	20.440252615928873	27.61949642746916	31.16415057396118	20.776100382640784
47	23.898289897685903	26.40486912888656	30.433714867900985	19.263126105526553
48	19.293325855300054	33.982683264360645	26.25187918772629	20.47211169261301
49	19.319875085870166	27.2653960647403	33.60037434415104	19.814354505238498
50	18.502822515074985	33.63223342083517	25.012361985484212	22.852582078605636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12.0
1	16.0
2	20.0
3	66.0
4	112.0
5	130.5
6	149.0
7	140.0
8	131.0
9	190.0
10	249.0
11	354.5
12	460.0
13	823.0
14	1186.0
15	1666.0
16	2146.0
17	2608.0
18	3070.0
19	3287.0
20	3504.0
21	3984.0
22	4464.0
23	4650.0
24	4836.0
25	4841.5
26	4847.0
27	6133.0
28	7419.0
29	8287.5
30	9156.0
31	9919.0
32	10682.0
33	12421.5
34	14161.0
35	15722.0
36	17283.0
37	18046.0
38	18809.0
39	17882.0
40	16955.0
41	17414.0
42	17873.0
43	18045.0
44	18217.0
45	23421.5
46	28626.0
47	33206.5
48	37787.0
49	32438.0
50	27089.0
51	20780.5
52	14472.0
53	11592.0
54	8712.0
55	7947.0
56	7182.0
57	6629.5
58	6077.0
59	5319.0
60	4561.0
61	3961.5
62	3362.0
63	2908.5
64	2455.0
65	2176.0
66	1897.0
67	1555.0
68	1213.0
69	1063.0
70	913.0
71	708.0
72	503.0
73	412.0
74	321.0
75	273.0
76	225.0
77	160.5
78	96.0
79	69.0
80	42.0
81	32.0
82	22.0
83	16.5
84	11.0
85	10.0
86	9.0
87	6.0
88	3.0
89	4.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016593269106319714
2	0.003650519203390337
3	0.0
4	3.3186538212639426E-4
5	0.0
6	9.955961463791827E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	301327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.932233603500244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.47235044656745	41.79108902013878
2	11.861342296016272	12.319719979355899
3	3.2910924449619112	5.127413449874303
4	1.3577392297795081	2.820417234141836
5	0.6758577796202986	1.7549402046992162
6	0.39827067395713345	1.24098514064192
7	0.27754966045489854	1.0089641662321174
8	0.21367162110980417	0.8877155633530356
9	0.16994862203697741	0.7943230386195552
>10	1.0667769979092296	10.763915523897756
>50	0.11988048380562599	4.294374597496697
>100	0.08269830166270456	7.801053226493729
>500	0.006410721059124385	2.210609142365925
>1k	0.005769648953211947	5.3254373254947796
>5k	6.410721059124385E-4	1.8590423871944768
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5584	1.8531362937937854	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4471	1.4837701234871086	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2203	0.7310994368244466	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2175	0.7218072061249076	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1512	0.5017804577751082	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1230	0.40819442001546496	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1181	0.3919330162912716	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1129	0.3746760164206991	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1080	0.35841461269650576	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1015	0.33684336285829014	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	876	0.2907140747427214	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	846	0.2807581132789295	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	725	0.24060240204163583	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	723	0.23993867127738303	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	690	0.22898711366721203	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	619	0.20542467153623806	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	608	0.20177415233284768	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	527	0.17489305638060976	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	515	0.17091067179509303	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	511	0.16958321026658746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.1513306142496358	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	419	0.1390515951109592	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	415	0.1377241335824536	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	364	0.1207989990940075	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	354	0.11748034527274356	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA	353	0.11714847989061716	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	351	0.11648474912636438	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	346	0.1148254222157324	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	345	0.11449355683360601	No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	341	0.11316609530510045	No Hit
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	328	0.1088518453374573	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG	317	0.10520132613406696	No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA	314	0.1042057299876878	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	310	0.10287826845918223	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	307	0.10188267231280304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	3.3186538212639426E-4	0.0
18	0.0	0.0	0.0	6.637307642527885E-4	0.0
19	0.0	0.0	0.0	6.637307642527885E-4	0.0
20	0.0	0.0	0.0	0.001327461528505577	0.0
21	0.0	0.0	0.0	0.00232305767488476	0.0
22	0.0	0.0	0.0	0.0033186538212639427	0.0
23	0.0	0.0	0.0	0.007632903788907068	0.0
24	0.0	0.0	0.0	0.010619692228044616	0.0
25	0.0	0.0	0.0	0.013606480667182164	0.0
26	0.0	0.0	0.0	0.017588865252698895	0.0
27	0.0	0.0	0.0	0.023894307513100385	0.0
28	0.0	0.0	0.0	0.036505192033903365	0.0
29	0.0	0.0	0.0	0.049447941936832746	0.0
30	0.0	0.0	0.0	0.07898396094608183	0.0
31	0.0	0.0	0.0	0.12942749902929376	0.0
32	0.0	0.0	0.0	0.1951368446903198	0.0
33	0.0	0.0	0.0	0.24723970968416373	0.0
34	0.0	0.0	0.0	0.3212456898983496	0.0
35	0.0	0.0	0.0	0.415827323804372	0.0
36	0.0	0.0	0.0	0.5206967845563126	0.0
37	0.0	0.0	0.0	0.6886206679122681	0.0
38	0.0	0.0	0.0	0.8724740896102905	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTGG	25	4.4350978E-5	43.999836	41
TCGTATA	25	0.002350043	35.199867	44
TGTATTG	35	3.2122468E-4	31.428452	5
TAGCTCG	35	3.2122468E-4	31.428452	36
GTATAGA	30	0.0057337102	29.338093	1
ATAGGAC	50	8.3212566E-5	26.3999	3
TAGGACC	150	0.0	26.3999	4
TGGTATA	235	0.0	26.212667	44
CGAATGT	130	0.0	25.38452	33
ATCCTAG	45	0.0013944629	24.44841	1
GTCGTAT	45	0.0013958123	24.444351	43
GTCCTAC	290	0.0	22.762312	1
TGTAGGA	340	0.0	22.646975	2
GATATAC	240	0.0	22.00357	1
GTCCTAA	150	0.0	22.003569	1
GTTTGGT	50	0.0025760424	21.999918	42
TCCTACA	290	0.0	21.999918	2
AGTGCTC	50	0.0025760424	21.999918	10
ATACACT	250	0.0	21.999918	4
GGTAGCT	50	0.0025760424	21.999918	34
>>END_MODULE