FastQCFastQC Report
Thu 2 Feb 2017
SRR4062476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127454
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG143991.277125275177524No Hit
TCCATGTACTCTGCGTTGATACCAC118001.046605892568566No Hit
GAGTACATGGAAGCAGTGGTATCAA56540.5014838742866672No Hit
CATGTACTCTGCGTTGATACCACTG46300.41065976971122553No Hit
GTATCAACGCAGAGTACTTTTTTTT38300.3397034380116617No Hit
GCTTCCATGTACTCTGCGTTGATAC36050.31974696972115935No Hit
CCCATGTACTCTGCGTTGATACCAC34020.301741800552395No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32230.2858653213346176No Hit
GTACATGGGAAGCAGTGGTATCAAC32000.2838253267982552No Hit
GCGTTGATACCACTGCTTCCATGTA31070.2755766532381809No Hit
CATGGAAGCAGTGGTATCAACGCAG30470.2702549283607136No Hit
TATCAACGCAGAGTACTTTTTTTTT27490.2438236948026261No Hit
GTTATAGCCTGTGGTGATGCGGCGC27320.24231587275401037No Hit
GTATCAACGCAGAGTACATGGAAGC24820.22014201909789669No Hit
CAAGTAGAGTGATCGAAAGATGAAA23730.2104742189038311No Hit
GGCTATAACACTCCCCGAGGGGAGC23290.20657162066035512No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21530.19096122768645107No Hit
CATCAGGATCGGTCGATGGTGCAGG19390.17198040895681774No Hit
ACTCTGCGTTGATACCACTGCTTCC19300.17118215022519767No Hit
ACGCAGAGTACATGGAAGCAGTGGT19100.16940824193270856No Hit
CTCTACTTGTGCGCTATCGGTCTCT19010.16860998320108847No Hit
CAGTGGTATCAACGCAGAGTACATG18620.16515086203073473No Hit
ATCCTGATGTCCTCGGATGGATTTG18020.15982913715326746No Hit
GTGTTATAGCCTGTGGTGATGCGGC16740.14847612408133726No Hit
CTTCTAGGTAGCGACCGGTCTAAGT15950.1414691863260053No Hit
TATCAACGCAGAGTACATGGAAGCA15790.14005005969201403No Hit
CTTTTGTGCCGTCTGGTGCGCTCCC15790.14005005969201403No Hit
GTCTAACATCTATGCGAGTGTTCGG15720.13942919178964286No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15550.13792136974102712No Hit
GTTCTAAGTTGGTTGTTAAGCGCGC15370.13632485227778696No Hit
GTATCAACGCAGAGTACATGGGAAG14990.13295442652205766No Hit
GTACATGGGGTGGTATCAACGCAAA14230.1262135750105991No Hit
ATCCTAAGCCATAGGGAAATTCCGT13820.12257706301099645No Hit
GGTATGGGTAGCACGCTTAAGCGCC13700.12151271803550301No Hit
ACCTAGAAGCATCCTCTACAAATTA13580.12044837306000955No Hit
ATGTTAGACTCCTTGGTCCGTGTTT13390.1187631601821449No Hit
CTATAACACTCCCCGAGGGGAGCTA13070.11592490691416234No Hit
GGTATCAACGCAGAGTACTTTTTTT12870.11415099862167326No Hit
GGTTAAGGTTGTTTCAACCCCAAGG12580.11157883159756407No Hit
GTGCTGGAAGGCGCCGGCCGTGAAG12450.11042579120744615No Hit
GCACAAAAGTGTGCCGCTCTACCAG12040.10678927920784352No Hit
TTCCAGCACCCCGTGGCGGACGCCC11550.10244320389124523No Hit
CTCCTTAGCGGATTCCGACTTCCAT11450.10155624974500067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT601.4650432E-615.83425914
TTATACC807.717972E-915.4356614
TCCTGAT2850.014.9935522
CCGTCTG2700.014.7792999
CTGGACT456.766396E-414.7760164
GCGAAAT653.371193E-614.61494212
CGGTCGA2750.014.50993810
GTACATA1058.913048E-1114.4686851
TATGGGT2300.014.4522313
TCCTCGG2900.014.41463510
TTAGGGT602.5699082E-514.2483024
GCCGTCT2550.014.15831858
CGATCCC759.645028E-713.93600517
CTTTAGA1500.013.9347679
TCGGTCG2800.013.9121459
TGATCGA3700.013.86567610
GTGATCG3500.013.844289
AGTGATC3600.013.7236338
TAATACT903.6408892E-813.7205874
AAACTTT2150.013.700317
Produced by FastQC (version 0.11.5)