##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062476_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127454
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998519673529916	32.0	32.0	32.0	32.0	32.0
2	30.648359046134033	32.0	32.0	32.0	32.0	32.0
3	30.65441073427386	32.0	32.0	32.0	32.0	32.0
4	30.71396527042345	32.0	32.0	32.0	32.0	32.0
5	30.492401463829122	32.0	32.0	32.0	32.0	32.0
6	34.12119962322188	36.0	36.0	36.0	32.0	36.0
7	33.9618574238949	36.0	36.0	36.0	32.0	36.0
8	33.941840642722454	36.0	36.0	36.0	32.0	36.0
9	34.127646005956784	36.0	36.0	36.0	32.0	36.0
10	33.78045756190497	36.0	36.0	36.0	32.0	36.0
11	34.16505063621221	36.0	36.0	36.0	32.0	36.0
12	33.925000044347705	36.0	36.0	36.0	32.0	36.0
13	34.03197558392626	36.0	36.0	36.0	32.0	36.0
14	33.93136039253043	36.0	36.0	36.0	32.0	36.0
15	33.879029210947856	36.0	36.0	36.0	32.0	36.0
16	33.84734632188985	36.0	36.0	36.0	32.0	36.0
17	33.7576344578138	36.0	36.0	36.0	32.0	36.0
18	33.7839202308919	36.0	36.0	36.0	32.0	36.0
19	33.752579706134355	36.0	36.0	36.0	32.0	36.0
20	33.74724822476128	36.0	36.0	36.0	32.0	36.0
21	33.70050662820834	36.0	36.0	36.0	27.0	36.0
22	33.64189847213279	36.0	36.0	36.0	27.0	36.0
23	33.56628740507373	36.0	36.0	36.0	27.0	36.0
24	33.51527955907736	36.0	36.0	36.0	27.0	36.0
25	33.00208789006026	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	44.0
5	104.0
6	194.0
7	48.0
8	167.0
9	180.0
10	152.0
11	40.0
12	79.0
13	68.0
14	258.0
15	326.0
16	561.0
17	721.0
18	1008.0
19	1535.0
20	2116.0
21	3148.0
22	4431.0
23	6558.0
24	8625.0
25	11736.0
26	15859.0
27	20498.0
28	26663.0
29	34945.0
30	44858.0
31	60817.0
32	86663.0
33	122085.0
34	253217.0
35	419743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.09771739641443	16.81868944332432	11.144335447725778	25.93925771253547
2	15.678731687154466	20.029554229616494	38.131697266056364	26.160016817172675
3	19.759481567290344	24.33337650057921	29.24504034954825	26.662101582582192
4	12.534562947810283	15.871412084368632	35.27429918273099	36.31972578509009
5	13.574520113784983	37.28960336741086	34.410019892851885	14.725856625952275
6	32.7748183761537	35.07738369374142	17.72144709858845	14.42635083151643
7	28.858282462328237	31.766760805392003	20.55292152522486	18.822035207054903
8	28.094619631460688	33.325669117144436	19.837418005463196	18.742293245931677
9	28.083749720337085	14.287489923256945	18.220970989633827	39.407789366772136
10	15.765651363111624	26.87026019003641	32.138975224225206	25.225113222626767
11	36.71072109488708	21.340956054797637	20.758773367006867	21.189549483308426
12	22.959682117887798	23.56983246177457	30.14267038026488	23.327815040072757
13	30.31165498163993	20.31143297353219	25.372773813699677	24.0041382311282
14	24.27733858119473	18.927912358820155	25.14151286892365	31.653236191061467
15	27.12441717878571	25.175301433222963	24.073279861623526	23.627001526367806
16	25.93035573326088	26.235361287292147	24.04935495986979	23.784928019577183
17	22.728580588211265	25.88951901234217	27.00473687856221	24.37716352088436
18	24.812946209324405	24.860281364424743	27.87490020097459	22.45187222527626
19	25.50659818772974	26.86637815044703	24.015112021321663	23.611911640501567
20	25.16892146894411	23.382425418363134	27.67807905942111	23.770574053271645
21	24.891051527054834	24.725597273868086	24.91369824447975	25.469652954597326
22	24.25699743286817	25.76849622924754	24.872309619193974	25.102196718690315
23	23.212534727896717	25.72058165230181	25.398438221647467	25.668445398154006
24	24.409641828505045	27.23058982973776	24.18468004003663	24.175088301720564
25	25.314055568630412	25.095049267536712	24.0963769821358	25.494518181697078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	263.0
2	632.5
3	1002.0
4	1002.0
5	1002.0
6	2632.0
7	4262.0
8	4262.0
9	4262.0
10	3955.0
11	3648.0
12	3648.0
13	3648.0
14	3011.0
15	2374.0
16	2374.0
17	2374.0
18	4354.0
19	6334.0
20	6334.0
21	6334.0
22	9997.0
23	13660.0
24	13660.0
25	13660.0
26	20224.5
27	26789.0
28	26789.0
29	26789.0
30	36352.5
31	45916.0
32	45916.0
33	45916.0
34	57095.5
35	68275.0
36	68275.0
37	68275.0
38	74825.5
39	81376.0
40	81376.0
41	81376.0
42	99766.0
43	118156.0
44	118156.0
45	118156.0
46	162902.0
47	207648.0
48	207648.0
49	207648.0
50	185181.0
51	162714.0
52	162714.0
53	162714.0
54	145674.5
55	128635.0
56	128635.0
57	128635.0
58	115161.5
59	101688.0
60	101688.0
61	101688.0
62	85129.0
63	68570.0
64	68570.0
65	68570.0
66	55161.0
67	41752.0
68	41752.0
69	41752.0
70	32463.5
71	23175.0
72	23175.0
73	23175.0
74	18274.0
75	13373.0
76	13373.0
77	13373.0
78	9296.0
79	5219.0
80	5219.0
81	5219.0
82	3275.0
83	1331.0
84	1331.0
85	1331.0
86	920.0
87	509.0
88	509.0
89	509.0
90	338.5
91	168.0
92	168.0
93	168.0
94	130.5
95	93.0
96	93.0
97	93.0
98	308.5
99	524.0
100	524.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008958236877069928
2	0.0031043395118559164
3	0.0049669432189694655
4	0.014457352583786123
5	0.03689729248377317
6	0.0476294376533322
7	0.06634417013909215
8	0.08931628252682593
9	0.09738756525765131
10	0.12009359140151173
11	0.11947272349914055
12	0.13322051276593103
13	0.12142402262087855
14	0.12488314379123229
15	0.11095796369519288
16	0.11042579120744615
17	0.12381879881573882
18	0.1278100924738393
19	0.1297613915955773
20	0.1187631601821449
21	0.1297613915955773
22	0.14936307822758177
23	0.1381874559849005
24	0.1317126907173153
25	0.12940660993707948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1127454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.238701947168096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8341399246513	31.32588021711905
2	12.233674919478569	9.600670477679305
3	3.460998621873685	4.074152800897832
4	1.3502545021190488	2.119289358458848
5	0.6608067170318094	1.296459890714891
6	0.3949483823446959	0.9298357115603826
7	0.2732661693063352	0.7505826838758963
8	0.1910428793809682	0.5997019682526878
9	0.13990564618391474	0.4940744356202927
>10	1.0406527876070704	8.509349252534586
>50	0.17605512703016366	4.8951915232286325
>100	0.20346974216783387	16.594130084787174
>500	0.02854920657722041	7.6232799689230575
>1k	0.011555631233636834	8.355432778963195
>5k	2.265810045811144E-4	0.5026826943493535
>10k+	4.531620091622288E-4	2.329286153034792
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	14399	1.277125275177524	No Hit
TCCATGTACTCTGCGTTGATACCAC	11800	1.046605892568566	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5654	0.5014838742866672	No Hit
CATGTACTCTGCGTTGATACCACTG	4630	0.41065976971122553	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3830	0.3397034380116617	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3605	0.31974696972115935	No Hit
CCCATGTACTCTGCGTTGATACCAC	3402	0.301741800552395	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3223	0.2858653213346176	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3200	0.2838253267982552	No Hit
GCGTTGATACCACTGCTTCCATGTA	3107	0.2755766532381809	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3047	0.2702549283607136	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2749	0.2438236948026261	No Hit
GTTATAGCCTGTGGTGATGCGGCGC	2732	0.24231587275401037	No Hit
GTATCAACGCAGAGTACATGGAAGC	2482	0.22014201909789669	No Hit
CAAGTAGAGTGATCGAAAGATGAAA	2373	0.2104742189038311	No Hit
GGCTATAACACTCCCCGAGGGGAGC	2329	0.20657162066035512	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2153	0.19096122768645107	No Hit
CATCAGGATCGGTCGATGGTGCAGG	1939	0.17198040895681774	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1930	0.17118215022519767	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1910	0.16940824193270856	No Hit
CTCTACTTGTGCGCTATCGGTCTCT	1901	0.16860998320108847	No Hit
CAGTGGTATCAACGCAGAGTACATG	1862	0.16515086203073473	No Hit
ATCCTGATGTCCTCGGATGGATTTG	1802	0.15982913715326746	No Hit
GTGTTATAGCCTGTGGTGATGCGGC	1674	0.14847612408133726	No Hit
CTTCTAGGTAGCGACCGGTCTAAGT	1595	0.1414691863260053	No Hit
TATCAACGCAGAGTACATGGAAGCA	1579	0.14005005969201403	No Hit
CTTTTGTGCCGTCTGGTGCGCTCCC	1579	0.14005005969201403	No Hit
GTCTAACATCTATGCGAGTGTTCGG	1572	0.13942919178964286	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1555	0.13792136974102712	No Hit
GTTCTAAGTTGGTTGTTAAGCGCGC	1537	0.13632485227778696	No Hit
GTATCAACGCAGAGTACATGGGAAG	1499	0.13295442652205766	No Hit
GTACATGGGGTGGTATCAACGCAAA	1423	0.1262135750105991	No Hit
ATCCTAAGCCATAGGGAAATTCCGT	1382	0.12257706301099645	No Hit
GGTATGGGTAGCACGCTTAAGCGCC	1370	0.12151271803550301	No Hit
ACCTAGAAGCATCCTCTACAAATTA	1358	0.12044837306000955	No Hit
ATGTTAGACTCCTTGGTCCGTGTTT	1339	0.1187631601821449	No Hit
CTATAACACTCCCCGAGGGGAGCTA	1307	0.11592490691416234	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1287	0.11415099862167326	No Hit
GGTTAAGGTTGTTTCAACCCCAAGG	1258	0.11157883159756407	No Hit
GTGCTGGAAGGCGCCGGCCGTGAAG	1245	0.11042579120744615	No Hit
GCACAAAAGTGTGCCGCTCTACCAG	1204	0.10678927920784352	No Hit
TTCCAGCACCCCGTGGCGGACGCCC	1155	0.10244320389124523	No Hit
CTCCTTAGCGGATTCCGACTTCCAT	1145	0.10155624974500067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.869541462445474E-5	0.0
12	0.0	0.0	0.0	1.7739082924890948E-4	0.0
13	0.0	1.7739082924890948E-4	0.0	1.7739082924890948E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCGAT	60	1.4650432E-6	15.834259	14
TTATACC	80	7.717972E-9	15.435661	4
TCCTGAT	285	0.0	14.993552	2
CCGTCTG	270	0.0	14.779299	9
CTGGACT	45	6.766396E-4	14.776016	4
GCGAAAT	65	3.371193E-6	14.614942	12
CGGTCGA	275	0.0	14.509938	10
GTACATA	105	8.913048E-11	14.468685	1
TATGGGT	230	0.0	14.452231	3
TCCTCGG	290	0.0	14.414635	10
TTAGGGT	60	2.5699082E-5	14.248302	4
GCCGTCT	255	0.0	14.1583185	8
CGATCCC	75	9.645028E-7	13.936005	17
CTTTAGA	150	0.0	13.934767	9
TCGGTCG	280	0.0	13.912145	9
TGATCGA	370	0.0	13.865676	10
GTGATCG	350	0.0	13.84428	9
AGTGATC	360	0.0	13.723633	8
TAATACT	90	3.6408892E-8	13.720587	4
AAACTTT	215	0.0	13.7003	17
>>END_MODULE