FastQCFastQC Report
Thu 2 Feb 2017
SRR4062476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127454
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA57820.5128368873585973No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC51260.454652695364955No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG27210.24134022319314136No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA25180.22333505402437706No Hit
GGCTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCG23850.21153856387932457No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20510.18191429539475668No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT19770.17535083471254703No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAA18800.16674737949397492No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18660.16550564368923254No Hit
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC16550.1467909112034726No Hit
CTTCTAGGTAGCGACCGGTCTAAGTTCCTTGGAACAGGACGTCATAGAGG15430.13685702476553369No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA15230.13508311647304458No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGG14570.12922921910783056No Hit
CTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCGGT14450.1281648741323371No Hit
GTGTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTC14440.12807617871771265No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG13910.12337532174261656No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT13820.12257706301099645No Hit
GGTATGGGTAGCACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTC13660.12115793637700518No Hit
ACCTAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAGATTTCA13660.12115793637700518No Hit
ATGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGCTGACAACCATTA13200.11707794730428027No Hit
GTGCTGGAAGGCGCCGGCCGTGAAGCTAGTGCCGACCTGCTCCCCGGGAA12800.11353013071930208No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12710.11273187198768198No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC12630.11202230867068635No Hit
ATCCTAAGCCATAGGGAAATTCCGTTTTAAAGTGCACACTCCGTGCCGCC12520.11104665910981736No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12020.10661188837859462No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG11910.10563623881772562No Hit
GCACAAAAGTGTGCCGCTCTACCAGTCGTTAAACCCTAGCTCCGGACAAA11870.10528145715922779No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11690.1036849396959876No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA11690.1036849396959876No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11530.10226581306199632No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA11390.10102407725725394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG301.3008094E-436.667962
CCCCGTA500.002580466721.9998027
ACGTCGC500.002580466721.9998028
TAGGACG4000.021.4498064
TAGGACA1950.021.4357034
AGAGTTA5950.021.0754440
TGGTATA4450.020.76385744
TGTAGGA5000.020.6807292
GTCCTAC4800.020.6321331
TCTATAC755.4726E-520.5331463
CCTTATA1201.4306352E-820.1673772
GTAGGAC5350.020.1493513
GCTATCG4100.019.85347713
GTTATAT1001.1195807E-619.8068491
ATACACT3350.019.7013134
CGCTTCG6600.019.66648943
ATTTCGT2800.019.6426842
GATTTCG2950.019.38965441
TATCGGT4200.019.38077515
GCGCTAT4100.019.31689811
Produced by FastQC (version 0.11.5)