##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1127454
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151684237228302	32.0	32.0	32.0	32.0	32.0
2	31.355552421650906	32.0	32.0	32.0	32.0	32.0
3	31.4596249603088	32.0	32.0	32.0	32.0	32.0
4	31.58654721168225	32.0	32.0	32.0	32.0	32.0
5	31.502662636347026	32.0	32.0	32.0	32.0	32.0
6	35.09532805773007	36.0	36.0	36.0	36.0	36.0
7	35.108338788101335	36.0	36.0	36.0	36.0	36.0
8	35.05429401110821	36.0	36.0	36.0	36.0	36.0
9	35.173571604695184	36.0	36.0	36.0	36.0	36.0
10	35.06612686637326	36.0	36.0	36.0	36.0	36.0
11	35.18659918719522	36.0	36.0	36.0	36.0	36.0
12	35.10276782910877	36.0	36.0	36.0	36.0	36.0
13	35.129225671291245	36.0	36.0	36.0	36.0	36.0
14	35.080782009731664	36.0	36.0	36.0	36.0	36.0
15	35.05512597409739	36.0	36.0	36.0	36.0	36.0
16	35.054825296641816	36.0	36.0	36.0	36.0	36.0
17	35.02843131515787	36.0	36.0	36.0	36.0	36.0
18	35.03842107970702	36.0	36.0	36.0	36.0	36.0
19	34.9942135111499	36.0	36.0	36.0	36.0	36.0
20	34.98765714610086	36.0	36.0	36.0	36.0	36.0
21	34.93531620802268	36.0	36.0	36.0	32.0	36.0
22	34.916127842022824	36.0	36.0	36.0	32.0	36.0
23	34.83988082884091	36.0	36.0	36.0	32.0	36.0
24	34.81652111749127	36.0	36.0	36.0	32.0	36.0
25	34.77582588735328	36.0	36.0	36.0	32.0	36.0
26	34.71435198243121	36.0	36.0	36.0	32.0	36.0
27	34.68415296765988	36.0	36.0	36.0	32.0	36.0
28	34.63363738121467	36.0	36.0	36.0	32.0	36.0
29	34.60559721283529	36.0	36.0	36.0	32.0	36.0
30	34.57923959647134	36.0	36.0	36.0	32.0	36.0
31	34.53956613750982	36.0	36.0	36.0	32.0	36.0
32	34.491886143470154	36.0	36.0	36.0	32.0	36.0
33	34.44961390886014	36.0	36.0	36.0	32.0	36.0
34	34.4175505164734	36.0	36.0	36.0	32.0	36.0
35	34.33348766335478	36.0	36.0	36.0	32.0	36.0
36	34.29884323440247	36.0	36.0	36.0	32.0	36.0
37	34.20376174992505	36.0	36.0	36.0	32.0	36.0
38	34.1495014430744	36.0	36.0	36.0	32.0	36.0
39	33.98141121500301	36.0	36.0	36.0	32.0	36.0
40	33.90233481809457	36.0	36.0	36.0	32.0	36.0
41	33.90162525477758	36.0	36.0	36.0	32.0	36.0
42	33.74853164741089	36.0	36.0	36.0	32.0	36.0
43	33.7670343978557	36.0	36.0	36.0	32.0	36.0
44	33.7623699060006	36.0	36.0	36.0	32.0	36.0
45	33.568759346279315	36.0	36.0	36.0	21.0	36.0
46	33.67157241004954	36.0	36.0	36.0	27.0	36.0
47	33.54997720527844	36.0	36.0	36.0	21.0	36.0
48	33.563094370147255	36.0	36.0	36.0	21.0	36.0
49	33.59599327333976	36.0	36.0	36.0	21.0	36.0
50	33.051218941083185	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	40.0
22	141.0
23	448.0
24	1123.0
25	2903.0
26	6432.0
27	11613.0
28	18955.0
29	26389.0
30	35647.0
31	48029.0
32	69438.0
33	94128.0
34	192537.0
35	619619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.369268872966686	17.488625345630705	11.844100038410804	26.298005742991805
2	15.250072954760952	20.42786525943068	37.61689392776903	26.705167858039346
3	19.23503345608844	24.35846265193605	29.67374105058504	26.732762841390468
4	12.470519783121395	16.35920319375299	35.21971243096152	35.95056459216409
5	13.374482129188534	37.365903501077206	34.35761845504868	14.901995914685578
6	33.19422874116701	35.41836482182343	17.18658671035231	14.20081972665726
7	28.76897860134427	31.611311858399542	20.52615893863519	19.093550601620997
8	28.110858624830815	33.49990332199806	19.680448160191013	18.708789892980114
9	27.964067713627337	13.990726007446867	18.278439741222257	39.76676653770353
10	15.7669403807162	26.904068813450483	32.308103035689264	25.020887770144057
11	37.21863597095758	20.898502289228652	20.77468349041291	21.108178249400865
12	23.018411394167746	23.422773789440633	30.142161010560077	23.416653805831547
13	30.617213651288655	19.955581336356072	25.6285400557362	23.798664956619074
14	24.087634617465547	18.68838994761649	25.322629570696453	31.901345864221515
15	27.24563485516926	25.18736906339416	23.97916012537984	23.587835956056743
16	25.869259411026967	25.914937549558566	24.156018782141	24.059784257273467
17	22.897164762376114	25.74579539386973	27.063365778116005	24.293674065638154
18	25.0877641127709	24.396383355773274	28.077065671858897	22.43878685959693
19	25.741449318553126	26.66326076274509	24.387070337237706	23.208219581464075
20	25.355890351180626	23.139924112203246	27.878476638514744	23.625708898101387
21	24.938755816201812	24.52454822990561	25.0304668749235	25.506229078969078
22	24.368799081825067	25.613284444420792	24.807930079630744	25.209986394123398
23	23.478297118995542	25.304358315283814	25.43651448307425	25.78083008264639
24	24.745754593979	27.137249058498174	24.222806429353216	23.894189918169612
25	25.658341715050014	24.612090604139947	24.131272761460778	25.598294919349257
26	23.210348271415064	24.576080265802418	26.05401195968971	26.15955950309281
27	25.182224729345943	25.90589061726687	26.046118067788132	22.865766585599058
28	24.327289627780825	24.181474366138218	25.563969793889598	25.927266212191363
29	23.238996890338765	25.97285565530833	26.423605752429815	24.364541701923095
30	25.1124214380365	24.861058632990794	26.303778247272174	23.72274168170054
31	23.75387377223372	24.736530270858058	25.588094946667443	25.92150101024077
32	23.52104830884453	25.27934620835972	25.850189896882707	25.349415585913036
33	23.89179514197475	25.30604352816168	26.062260633249785	24.739900696613788
34	23.31163843491619	25.765485775916357	27.17121940229934	23.75165638686811
35	24.659808737207904	25.13078138886376	25.82012215132502	24.389287722603317
36	23.75981636501356	26.027758116960868	26.16993686660387	24.0424886514217
37	25.693997271729046	25.82411344498312	24.774669299146574	23.707219984141258
38	25.3035600565522	25.617896605981265	24.02501565474068	25.053527682725857
39	24.528273437319836	24.689610396521722	26.17490380982284	24.6072123563356
40	24.564815948145114	24.865493403722013	25.273758397238378	25.29593225089449
41	23.467653669240608	25.21105073909889	26.44693264647604	24.874362945184462
42	25.609293150762692	25.416469319369128	25.601754040519616	23.372483489348568
43	25.312873075087765	24.30192273919823	24.040803438543833	26.34440074717017
44	24.16550919150582	24.569428109705584	27.419477867833187	23.84558483095541
45	25.28032185792059	24.96394531395516	25.169363894225395	24.586368933898857
46	24.116925494898677	24.81921641079495	26.22415302456067	24.8397050697457
47	24.889263774841368	24.816977011922436	26.6245008665542	23.669258346681993
48	24.000447024889706	27.71279360399626	24.033353023715378	24.253406347398652
49	23.916452467240347	25.27757230006723	27.65150507249076	23.154470160201658
50	22.72941351774933	27.989331687142055	24.33631262023804	24.944942174870572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	17.0
2	20.0
3	108.5
4	197.0
5	227.5
6	258.0
7	260.0
8	262.0
9	340.5
10	419.0
11	619.0
12	819.0
13	1475.0
14	2131.0
15	2957.5
16	3784.0
17	4374.5
18	4965.0
19	5315.5
20	5666.0
21	6096.0
22	6526.0
23	6695.5
24	6865.0
25	6522.5
26	6180.0
27	8085.0
28	9990.0
29	11250.0
30	12510.0
31	14328.0
32	16146.0
33	20108.0
34	24070.0
35	27820.0
36	31570.0
37	34212.0
38	36854.0
39	39464.0
40	42074.0
41	48647.0
42	55220.0
43	59602.0
44	63984.0
45	75701.5
46	87419.0
47	95908.5
48	104398.0
49	105875.0
50	107352.0
51	99237.0
52	91122.0
53	84398.0
54	77674.0
55	76159.0
56	74644.0
57	68217.0
58	61790.0
59	58872.5
60	55955.0
61	50009.5
62	44064.0
63	35826.5
64	27589.0
65	25609.0
66	23629.0
67	18486.0
68	13343.0
69	12027.5
70	10712.0
71	8532.0
72	6352.0
73	5307.0
74	4262.0
75	3808.0
76	3354.0
77	2509.0
78	1664.0
79	1246.0
80	828.0
81	585.5
82	343.0
83	275.0
84	207.0
85	167.0
86	127.0
87	97.5
88	68.0
89	42.0
90	16.0
91	13.5
92	11.0
93	7.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014812134242283944
2	0.0038139028288515543
3	5.321724877467284E-4
4	2.660862438733642E-4
5	8.869541462445474E-5
6	4.4347707312227376E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.869541462445474E-5
47	0.0
48	0.0
49	0.0
50	2.660862438733642E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1127454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.658739491422075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2054459317863	35.81874115660983
2	12.032432765667057	10.747065606599527
3	3.377289987034837	4.524765411539311
4	1.3075460724257975	2.3357343768598318
5	0.6997334884691885	1.562460778748474
6	0.396472243300497	1.0623570377481966
7	0.272202631315302	0.8509358480552777
8	0.18865330470852684	0.6740015031339176
9	0.15790067085023812	0.634648043251941
>10	1.014432539617043	9.200935521140854
>50	0.15120846360523355	4.766009261514482
>100	0.16696019412646765	15.486424195490695
>500	0.021343776234991358	6.354277341554333
>1k	0.007978981770090227	5.00992854916128
>5k	3.9894908850451134E-4	0.9717153685921024
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5782	0.5128368873585973	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5126	0.454652695364955	No Hit
GTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTCGG	2721	0.24134022319314136	No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA	2518	0.22333505402437706	No Hit
GGCTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCG	2385	0.21153856387932457	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2051	0.18191429539475668	No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT	1977	0.17535083471254703	No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGAAA	1880	0.16674737949397492	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1866	0.16550564368923254	No Hit
ATCCTGATGTCCTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC	1655	0.1467909112034726	No Hit
CTTCTAGGTAGCGACCGGTCTAAGTTCCTTGGAACAGGACGTCATAGAGG	1543	0.13685702476553369	No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA	1523	0.13508311647304458	No Hit
GTTCTAAGTTGGTTGTTAAGCGCGCGCCGGACGGCCGAAGCCTGCCAAGG	1457	0.12922921910783056	No Hit
CTATAACACTCCCCGAGGGGAGCTACATTCCTAAGGTCTTATCCGGCGGT	1445	0.1281648741323371	No Hit
GTGTTATAGCCTGTGGTGATGCGGCGCGTCTCGGGTGAGGTCCGCGCTTC	1444	0.12807617871771265	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1391	0.12337532174261656	No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT	1382	0.12257706301099645	No Hit
GGTATGGGTAGCACGCTTAAGCGCCATCCATTTTCAGGGCTAGTTCATTC	1366	0.12115793637700518	No Hit
ACCTAGAAGCATCCTCTACAAATTACAACTCGGGCTTGCGCCAGATTTCA	1366	0.12115793637700518	No Hit
ATGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGCTGACAACCATTA	1320	0.11707794730428027	No Hit
GTGCTGGAAGGCGCCGGCCGTGAAGCTAGTGCCGACCTGCTCCCCGGGAA	1280	0.11353013071930208	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1271	0.11273187198768198	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1263	0.11202230867068635	No Hit
ATCCTAAGCCATAGGGAAATTCCGTTTTAAAGTGCACACTCCGTGCCGCC	1252	0.11104665910981736	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1202	0.10661188837859462	No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG	1191	0.10563623881772562	No Hit
GCACAAAAGTGTGCCGCTCTACCAGTCGTTAAACCCTAGCTCCGGACAAA	1187	0.10528145715922779	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1169	0.1036849396959876	No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA	1169	0.1036849396959876	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1153	0.10226581306199632	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1139	0.10102407725725394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.869541462445474E-5	0.0
12	0.0	0.0	0.0	1.7739082924890948E-4	0.0
13	0.0	0.0	0.0	1.7739082924890948E-4	0.0
14	0.0	0.0	0.0	1.7739082924890948E-4	0.0
15	0.0	0.0	0.0	1.7739082924890948E-4	0.0
16	0.0	0.0	0.0	3.5478165849781897E-4	0.0
17	0.0	0.0	0.0	6.208679023711832E-4	0.0
18	0.0	0.0	0.0	0.001064344975493457	0.0
19	0.0	0.0	0.0	0.0012417358047423664	0.0
20	0.0	0.0	0.0	0.0018626037071135496	0.0
21	0.0	0.0	0.0	0.0023060807802358233	0.0
22	0.0	0.0	0.0	0.0039025982434760086	0.0
23	0.0	0.0	0.0	0.006297374438336287	0.0
24	0.0	0.0	0.0	0.010377363511061204	0.0
25	0.0	0.0	0.0	0.013038225949794847	0.0
26	0.0	0.0	0.0	0.015787783803152943	0.0
27	0.0	0.0	0.0	0.02039994536362459	0.0
28	0.0	0.0	0.0	0.03042252721618798	0.0
29	0.0	0.0	0.0	0.04230771277586491	0.0
30	0.0	0.0	0.0	0.0635059168711096	0.0
31	0.0	0.0	0.0	0.10918405540270379	0.0
32	0.0	0.0	0.0	0.16515086203073473	0.0
33	0.0	8.869541462445474E-5	0.0	0.21464290339118047	0.0
34	0.0	8.869541462445474E-5	0.0	0.282228809335015	0.0
35	0.0	1.7739082924890948E-4	0.0	0.3533625318638277	0.0
36	0.0	2.660862438733642E-4	0.0	0.4505727062922301	0.0
37	0.0	2.660862438733642E-4	0.0	0.5995810028613141	0.0
38	0.0	2.660862438733642E-4	0.0	0.781938775329193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	30	1.3008094E-4	36.66796	2
CCCCGTA	50	0.0025804667	21.999802	7
ACGTCGC	50	0.0025804667	21.999802	8
TAGGACG	400	0.0	21.449806	4
TAGGACA	195	0.0	21.435703	4
AGAGTTA	595	0.0	21.07544	40
TGGTATA	445	0.0	20.763857	44
TGTAGGA	500	0.0	20.680729	2
GTCCTAC	480	0.0	20.632133	1
TCTATAC	75	5.4726E-5	20.533146	3
CCTTATA	120	1.4306352E-8	20.167377	2
GTAGGAC	535	0.0	20.149351	3
GCTATCG	410	0.0	19.853477	13
GTTATAT	100	1.1195807E-6	19.806849	1
ATACACT	335	0.0	19.701313	4
CGCTTCG	660	0.0	19.666489	43
ATTTCGT	280	0.0	19.64268	42
GATTTCG	295	0.0	19.389654	41
TATCGGT	420	0.0	19.380775	15
GCGCTAT	410	0.0	19.316898	11
>>END_MODULE