FastQCFastQC Report
Thu 2 Feb 2017
SRR4062475_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062475_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047826
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53860.514016640167356No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41880.39968468047175776No Hit
GTACATGGAAGCAGTGGTATCAACG40100.3826971271947823No Hit
TATCAACGCAGAGTACTTTTTTTTT35740.3410871652354494No Hit
TCCATGTACTCTGCGTTGATACCAC34200.3263900685800887No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28880.2756182801342971No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21010.20051039008384977No Hit
GTACATGGGGTGGTATCAACGCAAA16400.15651453581033492No Hit
GAGTACATGGAAGCAGTGGTATCAA16300.15556017888466214No Hit
GGTATCAACGCAGAGTACTTTTTTT15140.14448963854685798No Hit
CATGTACTCTGCGTTGATACCACTG13550.12931536342866087No Hit
GTACATGGGTGGTATCAACGCAAAA10870.10373859782063052No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10700.10211619104698681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT456.785927E-414.7702382
AATTCCG759.667583E-713.9328785
TCGCGTA551.9549718E-413.8216929
TTCCGAT707.248831E-613.572937
GTCTTAA655.4777785E-513.1465071
CGCGTAA604.0829307E-412.6692810
CGTCAAT751.4758107E-512.66806819
GTATAGA604.1093442E-412.6595991
GTTCTAG1001.4511716E-712.3431081
TATACTG853.941277E-612.29371555
CCGATAA802.8568285E-511.8780169
ATTCCGA802.8610237E-511.8763146
TAACGAA802.8638235E-511.87517913
TTAACCA658.031428E-411.690254
CCCCTAT658.0572156E-411.6857841
CGAACCT1552.5465852E-1111.64589316
TCCGTCA907.444758E-611.61239617
TCCAGTA907.49641E-611.6051872
CCTATAC752.0805834E-411.3952723
CGATAAC855.3076117E-511.17877710