##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062475_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1047826
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.999457925266217	32.0	32.0	32.0	32.0	32.0
2	30.723623960466718	32.0	32.0	32.0	32.0	32.0
3	30.738999604896232	32.0	32.0	32.0	32.0	32.0
4	30.748668194910223	32.0	32.0	32.0	32.0	32.0
5	30.622192997692366	32.0	32.0	32.0	32.0	32.0
6	34.23432039288966	36.0	36.0	36.0	32.0	36.0
7	34.053189174538524	36.0	36.0	36.0	32.0	36.0
8	34.03493709833503	36.0	36.0	36.0	32.0	36.0
9	34.15014038590377	36.0	36.0	36.0	32.0	36.0
10	33.88504866265964	36.0	36.0	36.0	32.0	36.0
11	34.22559757058901	36.0	36.0	36.0	32.0	36.0
12	34.01330850732851	36.0	36.0	36.0	32.0	36.0
13	34.11596581875235	36.0	36.0	36.0	32.0	36.0
14	34.03371265839939	36.0	36.0	36.0	32.0	36.0
15	33.99963829872517	36.0	36.0	36.0	32.0	36.0
16	33.98894186630223	36.0	36.0	36.0	32.0	36.0
17	33.92691725534583	36.0	36.0	36.0	32.0	36.0
18	33.94604065942246	36.0	36.0	36.0	32.0	36.0
19	33.9181171301342	36.0	36.0	36.0	32.0	36.0
20	33.87033247886576	36.0	36.0	36.0	32.0	36.0
21	33.82468749582469	36.0	36.0	36.0	32.0	36.0
22	33.79092521086516	36.0	36.0	36.0	32.0	36.0
23	33.77529570749342	36.0	36.0	36.0	32.0	36.0
24	33.76671031259007	36.0	36.0	36.0	32.0	36.0
25	33.30972317923014	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	50.0
5	99.0
6	182.0
7	49.0
8	182.0
9	187.0
10	134.0
11	35.0
12	77.0
13	66.0
14	314.0
15	391.0
16	520.0
17	763.0
18	1147.0
19	1533.0
20	2282.0
21	3185.0
22	4496.0
23	6277.0
24	8312.0
25	10700.0
26	14125.0
27	17510.0
28	22816.0
29	29444.0
30	36795.0
31	49842.0
32	69788.0
33	100461.0
34	224732.0
35	441318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.86964198791673	17.429585866587765	11.28745001097611	25.41332213451939
2	16.44726858696057	20.718921998757388	37.258002788724085	25.575806625557963
3	19.517847138511815	25.221162500155092	27.850488518829238	27.41050184250386
4	12.892624618077795	15.79833553029081	35.23611168116404	36.07292817046736
5	14.473015909386003	36.894710110059656	33.036034797485655	15.596239183068686
6	33.1807834944448	34.89813884740688	17.702624251891518	14.218453406256803
7	29.3990510427451	30.901853217247794	21.141613914983516	18.55748182502359
8	28.47796307724052	31.96992073241794	20.121432815518027	19.430683374823516
9	28.134377194281374	14.45247455230689	18.847008134663795	38.56614011874794
10	16.306538305343878	26.96227992642316	31.39026779102267	25.34091397721029
11	36.591434869980766	21.653436840360097	22.11201348496272	19.64311480469642
12	24.923352753184396	24.002438949798922	28.894769637750965	22.179438659265717
13	30.242438315402016	20.04781685113995	25.198257417870927	24.51148741558711
14	23.942984975073127	19.570055644691966	25.729864753679305	30.7570946265556
15	25.238256309707403	26.8186691280593	23.73543086792756	24.207643694305737
16	25.045074656243045	26.000647813819455	24.688012673455017	24.266264856482486
17	23.48026015278147	25.6000695677435	26.300907247468135	24.618763032006896
18	24.283383871155788	24.882383690538422	27.639808832284828	23.194423606020962
19	25.066897814543964	25.396608471939235	26.387747378561354	23.148746334955447
20	25.509522521808016	24.01160418492163	26.763298134099813	23.715575159170545
21	26.26754358300984	24.316270926629933	24.978163101088118	24.43802238927211
22	25.242736078639034	25.00004779210054	25.640175186723734	24.11704094253669
23	23.850361318427574	25.067690457963103	26.012155132289088	25.069793091320236
24	24.00717156908656	25.677638734118663	26.017963327898592	24.297226368896187
25	24.331688017545787	24.945121630726153	26.07298321395636	24.650207137771705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	386.0
1	386.0
2	879.0
3	1372.0
4	1372.0
5	1372.0
6	3433.5
7	5495.0
8	5495.0
9	5495.0
10	5275.0
11	5055.0
12	5055.0
13	5055.0
14	4468.5
15	3882.0
16	3882.0
17	3882.0
18	6291.0
19	8700.0
20	8700.0
21	8700.0
22	13499.0
23	18298.0
24	18298.0
25	18298.0
26	26755.0
27	35212.0
28	35212.0
29	35212.0
30	43332.5
31	51453.0
32	51453.0
33	51453.0
34	59155.0
35	66857.0
36	66857.0
37	66857.0
38	75065.5
39	83274.0
40	83274.0
41	83274.0
42	95461.5
43	107649.0
44	107649.0
45	107649.0
46	125945.0
47	144241.0
48	144241.0
49	144241.0
50	139491.5
51	134742.0
52	134742.0
53	134742.0
54	121199.5
55	107657.0
56	107657.0
57	107657.0
58	98254.5
59	88852.0
60	88852.0
61	88852.0
62	79663.5
63	70475.0
64	70475.0
65	70475.0
66	60419.5
67	50364.0
68	50364.0
69	50364.0
70	39268.5
71	28173.0
72	28173.0
73	28173.0
74	22703.5
75	17234.0
76	17234.0
77	17234.0
78	13601.0
79	9968.0
80	9968.0
81	9968.0
82	7359.5
83	4751.0
84	4751.0
85	4751.0
86	3520.0
87	2289.0
88	2289.0
89	2289.0
90	1516.0
91	743.0
92	743.0
93	743.0
94	467.0
95	191.0
96	191.0
97	191.0
98	352.0
99	513.0
100	513.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00916182648645863
2	0.003340249239854709
3	0.006394191402007586
4	0.01574688927360077
5	0.03760166287150729
6	0.04943568874984968
7	0.07463071158761092
8	0.09467220702673917
9	0.10221162673955408
10	0.12416183603002788
11	0.12664316403677708
12	0.1406722108441669
13	0.12721577819218075
14	0.13026972035433365
15	0.12063071540503861
16	0.11729046616518392
17	0.13017428466176637
18	0.1350415049826975
19	0.13876349699282134
20	0.12473445018543156
21	0.13666391175634124
22	0.1553693074995276
23	0.14496681700969435
24	0.14048133945903232
25	0.13561411913810117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1047826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.01774346548707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37689178080805	43.90496617399548
2	13.669026133876386	15.31415998781052
3	3.8854630494814697	6.529646170514461
4	1.4313641114508233	3.207271504038282
5	0.7113312464105275	1.9923585640205048
6	0.39437275955077117	1.325512324457479
7	0.2705215670482336	1.0607805421352656
8	0.17529891305932035	0.7855879633228584
9	0.13151290101053736	0.663035035608925
>10	0.7751740519234177	8.359994721488793
>50	0.09963002868499024	3.944446831703968
>100	0.07239631980691631	7.870312465967512
>500	0.00479219104590428	1.7953400595160838
>1k	0.0020537961625304055	2.7302092745295057
>5k	1.7114968021086712E-4	0.5163783808903999
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5386	0.514016640167356	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4188	0.39968468047175776	No Hit
GTACATGGAAGCAGTGGTATCAACG	4010	0.3826971271947823	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3574	0.3410871652354494	No Hit
TCCATGTACTCTGCGTTGATACCAC	3420	0.3263900685800887	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2888	0.2756182801342971	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2101	0.20051039008384977	No Hit
GTACATGGGGTGGTATCAACGCAAA	1640	0.15651453581033492	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1630	0.15556017888466214	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1514	0.14448963854685798	No Hit
CATGTACTCTGCGTTGATACCACTG	1355	0.12931536342866087	No Hit
GTACATGGGTGGTATCAACGCAAAA	1087	0.10373859782063052	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1070	0.10211619104698681	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.863070777018322E-4	0.0	0.0	0.0
6	0.0	2.863070777018322E-4	0.0	0.0	0.0
7	0.0	3.817427702691096E-4	0.0	0.0	0.0
8	0.0	3.817427702691096E-4	0.0	0.0	0.0
9	0.0	3.817427702691096E-4	0.0	9.54356925672774E-5	0.0
10	9.54356925672774E-5	3.817427702691096E-4	0.0	9.54356925672774E-5	0.0
11	9.54356925672774E-5	3.817427702691096E-4	0.0	9.54356925672774E-5	0.0
12	9.54356925672774E-5	4.77178462836387E-4	0.0	9.54356925672774E-5	0.0
13	9.54356925672774E-5	4.77178462836387E-4	0.0	1.908713851345548E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	45	6.785927E-4	14.770238	2
AATTCCG	75	9.667583E-7	13.932878	5
TCGCGTA	55	1.9549718E-4	13.821692	9
TTCCGAT	70	7.248831E-6	13.57293	7
GTCTTAA	65	5.4777785E-5	13.146507	1
CGCGTAA	60	4.0829307E-4	12.66928	10
CGTCAAT	75	1.4758107E-5	12.668068	19
GTATAGA	60	4.1093442E-4	12.659599	1
GTTCTAG	100	1.4511716E-7	12.343108	1
TATACTG	85	3.941277E-6	12.2937155	5
CCGATAA	80	2.8568285E-5	11.878016	9
ATTCCGA	80	2.8610237E-5	11.876314	6
TAACGAA	80	2.8638235E-5	11.875179	13
TTAACCA	65	8.031428E-4	11.69025	4
CCCCTAT	65	8.0572156E-4	11.685784	1
CGAACCT	155	2.5465852E-11	11.645893	16
TCCGTCA	90	7.444758E-6	11.612396	17
TCCAGTA	90	7.49641E-6	11.605187	2
CCTATAC	75	2.0805834E-4	11.395272	3
CGATAAC	85	5.3076117E-5	11.178777	10
>>END_MODULE