FastQCFastQC Report
Thu 2 Feb 2017
SRR4062475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047826
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15970.15241080102994198No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14560.13895436837795588No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13790.13160582005027552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11540.11013278922263811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT752.0596708E-623.4721151
ATGCGTT500.002580308621.99985740
GGCGTTA2250.019.55542842
CGCGAAG901.0018071E-519.55542831
CCGACCA2800.018.071319
CGACCAT2450.017.95906610
ACGCTTA1509.591531E-917.59988617
TCGACGC1509.591531E-917.59988614
CGCAATA2550.017.2547936
CCCTTAT902.2110928E-417.1150841
AGCGCGT1451.2488454E-716.68954732
GTCTAGA2007.2759576E-1116.503831
TACACCG800.00198819916.4998935
ATGGGTG8200.016.3657475
GACGCTT1501.8370338E-716.13322816
CGGTCCA3000.016.13322810
CGCTTAC1653.1090167E-815.99989718
TAGCGGC2750.015.99989630
CGTTATT2750.015.99989644
ATAGCAC1805.2623363E-915.8887843
Produced by FastQC (version 0.11.5)