FastQCFastQC Report
Thu 2 Feb 2017
SRR4062474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978413
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33590.16978254793109426No Hit
TATCAACGCAGAGTACTTTTTTTTT21250.10740932252264822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG2250.012.2440857
CGTCGTA3700.012.06751910
CAAGACG3800.011.7487634
CGAGCCG4300.011.71073715
GCGTTAT1552.5465852E-1111.6386341
AAGGCGT907.465753E-611.6107716
AAGACGG3850.011.5964765
CGCCAGT3800.011.50344818
TTAATAC1001.9374183E-611.3964933
ACCGTCG3600.011.3471778
GATTAAA2550.011.1702071
CGCGGTT2750.011.05450210
GGTCCAA4300.011.04646811
CCGTCGT3800.010.9999579
CGGTCGG2250.010.97773410
CGCATCG3900.010.96288213
CGCAAGA3650.010.9275922
ATACCGT4350.010.919226
GCATCGC3950.010.82411114
CGGTCCA4050.010.7900810
Produced by FastQC (version 0.11.5)