##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062474_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1978413
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21919336356969	32.0	32.0	32.0	32.0	32.0
2	30.920935113143717	32.0	32.0	32.0	32.0	32.0
3	30.89910802244021	32.0	32.0	32.0	32.0	32.0
4	30.973888161875198	32.0	32.0	32.0	32.0	32.0
5	30.794414007590934	32.0	32.0	32.0	32.0	32.0
6	34.440735579477085	36.0	36.0	36.0	32.0	36.0
7	34.33222689094744	36.0	36.0	36.0	32.0	36.0
8	34.348118416124436	36.0	36.0	36.0	32.0	36.0
9	34.48299419787476	36.0	36.0	36.0	32.0	36.0
10	34.18973793641671	36.0	36.0	36.0	32.0	36.0
11	34.461919730612365	36.0	36.0	36.0	32.0	36.0
12	34.264122304089184	36.0	36.0	36.0	32.0	36.0
13	34.37295953878184	36.0	36.0	36.0	32.0	36.0
14	34.28400440150767	36.0	36.0	36.0	32.0	36.0
15	34.22329008149461	36.0	36.0	36.0	32.0	36.0
16	34.233151015485646	36.0	36.0	36.0	32.0	36.0
17	34.15869436765731	36.0	36.0	36.0	32.0	36.0
18	34.1689030551255	36.0	36.0	36.0	32.0	36.0
19	34.15706275686624	36.0	36.0	36.0	32.0	36.0
20	34.1489850703569	36.0	36.0	36.0	32.0	36.0
21	34.13118191196681	36.0	36.0	36.0	32.0	36.0
22	34.08674225250238	36.0	36.0	36.0	32.0	36.0
23	34.051059611921275	36.0	36.0	36.0	32.0	36.0
24	34.02679319232132	36.0	36.0	36.0	32.0	36.0
25	33.58232583388807	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	65.0
5	173.0
6	364.0
7	88.0
8	257.0
9	328.0
10	257.0
11	68.0
12	160.0
13	113.0
14	266.0
15	396.0
16	555.0
17	823.0
18	1035.0
19	1461.0
20	2210.0
21	3331.0
22	4968.0
23	7396.0
24	10313.0
25	15000.0
26	21228.0
27	27794.0
28	38100.0
29	51229.0
30	65854.0
31	92047.0
32	132656.0
33	194955.0
34	446859.0
35	858054.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.347219624538354	17.270143732516182	11.105578746553785	26.277057896391675
2	17.07871858937064	19.880900810826795	36.58031360461314	26.46006699518943
3	19.338027528268793	23.45063765903565	28.07443651969085	29.136898293004705
4	13.07243630834063	15.180949222596762	35.33140490687136	36.41520956219125
5	15.168020338705379	35.96923103371733	33.19627224861924	15.666476378958055
6	34.57176074005262	34.68270997294032	16.844055644529583	13.901473642477475
7	30.551037253735693	30.157034591309483	20.61964126032297	18.672286894631853
8	28.332776005176324	32.41332872631208	19.53692353845539	19.71697173005621
9	27.448356463094413	14.522420605809168	18.516086272406692	39.513136658689724
10	16.176227985923948	26.43409668026569	31.342295667980014	26.04737966583035
11	37.48926551042023	21.087825709842637	21.979680250107915	19.443228529629216
12	25.018422245975447	23.541555323421758	28.329466686101124	23.110555744501667
13	29.540006467306956	19.353998015275597	25.050238020174152	26.0557574972433
14	23.685799319943058	19.62428346805762	24.78710897864435	31.90280823335498
15	25.472303901848836	26.730161718070434	22.135726997591863	25.661807382488867
16	25.97837037531796	25.53435232313787	23.55360170745814	24.933675594086033
17	24.342318343022782	25.47261380382504	24.95318901718191	25.231878835970267
18	25.095499817806388	24.70661767682902	25.885562168508848	24.312320336855745
19	25.667531436912093	24.889035772741213	25.069918850607987	24.373513939738707
20	25.83128293831811	24.231063914633395	24.781153437022088	25.15649971002641
21	26.707711773265007	24.1408996014424	24.18913139748213	24.962257227810465
22	25.904785431101864	24.399897544717884	24.511616323191745	25.18370070098851
23	24.726650800383464	24.168209596907996	25.139926647965915	25.965212954742622
24	24.889222255457653	24.93214151410844	24.914477809781644	25.26415842065226
25	24.99845638555339	24.490683148082727	25.039653177669518	25.471207288694366
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	214.0
1	214.0
2	583.5
3	953.0
4	953.0
5	953.0
6	2314.5
7	3676.0
8	3676.0
9	3676.0
10	3965.5
11	4255.0
12	4255.0
13	4255.0
14	4916.5
15	5578.0
16	5578.0
17	5578.0
18	8991.5
19	12405.0
20	12405.0
21	12405.0
22	20103.0
23	27801.0
24	27801.0
25	27801.0
26	40937.5
27	54074.0
28	54074.0
29	54074.0
30	67363.0
31	80652.0
32	80652.0
33	80652.0
34	100825.5
35	120999.0
36	120999.0
37	120999.0
38	137766.5
39	154534.0
40	154534.0
41	154534.0
42	177533.5
43	200533.0
44	200533.0
45	200533.0
46	227209.5
47	253886.0
48	253886.0
49	253886.0
50	258492.0
51	263098.0
52	263098.0
53	263098.0
54	247642.5
55	232187.0
56	232187.0
57	232187.0
58	214174.5
59	196162.0
60	196162.0
61	196162.0
62	173715.5
63	151269.0
64	151269.0
65	151269.0
66	125739.0
67	100209.0
68	100209.0
69	100209.0
70	76408.0
71	52607.0
72	52607.0
73	52607.0
74	41554.0
75	30501.0
76	30501.0
77	30501.0
78	24672.5
79	18844.0
80	18844.0
81	18844.0
82	13390.0
83	7936.0
84	7936.0
85	7936.0
86	5785.0
87	3634.0
88	3634.0
89	3634.0
90	2451.5
91	1269.0
92	1269.0
93	1269.0
94	776.0
95	283.0
96	283.0
97	283.0
98	568.5
99	854.0
100	854.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00783456234871081
2	0.003133824939484324
3	0.0041952817738257885
4	0.014607667863080157
5	0.035685167859289234
6	0.047108465219345
7	0.0641423201323485
8	0.08693836928891996
9	0.09517729614595133
10	0.11579988607029977
11	0.11655806952340082
12	0.1284362769553172
13	0.1172657074129618
14	0.12141044362324752
15	0.11099805753399315
16	0.10826859710282939
17	0.12034898678890606
18	0.12601008990539386
19	0.12894173259071792
20	0.11984353115350536
21	0.128284640264697
22	0.14744140884638343
23	0.13824211628209074
24	0.13192392083958204
25	0.1279308213199165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1978413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38009939315637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9244127867529	44.26221924070033
2	12.298677126858298	13.622039233795025
3	3.5370814658307	5.876517694181862
4	1.390603947393135	3.0804713929258964
5	0.7419551077636704	2.054477380660605
6	0.4498616292735973	1.4948029045403446
7	0.3051572156184537	1.1829745852042168
8	0.22202015256312735	0.9836398492983786
9	0.15053533593978574	0.7502995679874767
>10	0.8129240015921697	8.254727947370208
>50	0.07735861427752935	3.0056488117783458
>100	0.07577795169266617	8.960003824226067
>500	0.01006585965653164	3.653364180954895
>1k	0.0035688047873157634	2.818813386376304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3359	0.16978254793109426	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2125	0.10740932252264822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0109112708013949E-4
2	0.0	0.0	0.0	0.0	1.0109112708013949E-4
3	0.0	0.0	0.0	0.0	1.0109112708013949E-4
4	0.0	0.0	0.0	0.0	1.0109112708013949E-4
5	0.0	0.0	0.0	0.0	1.5163669062020924E-4
6	0.0	0.0	0.0	0.0	2.0218225416027897E-4
7	0.0	0.0	0.0	0.0	2.0218225416027897E-4
8	0.0	0.0	0.0	0.0	2.5272781770034874E-4
9	0.0	0.0	0.0	5.0545563540069743E-5	2.5272781770034874E-4
10	0.0	0.0	0.0	1.5163669062020924E-4	2.5272781770034874E-4
11	0.0	0.0	0.0	1.5163669062020924E-4	2.5272781770034874E-4
12	0.0	0.0	0.0	1.5163669062020924E-4	3.538189447804882E-4
13	0.0	0.0	0.0	2.0218225416027897E-4	4.0436450832055795E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	225	0.0	12.244085	7
CGTCGTA	370	0.0	12.067519	10
CAAGACG	380	0.0	11.748763	4
CGAGCCG	430	0.0	11.710737	15
GCGTTAT	155	2.5465852E-11	11.638634	1
AAGGCGT	90	7.465753E-6	11.610771	6
AAGACGG	385	0.0	11.596476	5
CGCCAGT	380	0.0	11.503448	18
TTAATAC	100	1.9374183E-6	11.396493	3
ACCGTCG	360	0.0	11.347177	8
GATTAAA	255	0.0	11.170207	1
CGCGGTT	275	0.0	11.054502	10
GGTCCAA	430	0.0	11.046468	11
CCGTCGT	380	0.0	10.999957	9
CGGTCGG	225	0.0	10.977734	10
CGCATCG	390	0.0	10.962882	13
CGCAAGA	365	0.0	10.927592	2
ATACCGT	435	0.0	10.91922	6
GCATCGC	395	0.0	10.824111	14
CGGTCCA	405	0.0	10.79008	10
>>END_MODULE