FastQCFastQC Report
Thu 2 Feb 2017
SRR4062474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978413
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2650.019.0941664
GTATCAA36150.018.8109861
TTAACGG2250.017.59983835
GTATTAG4500.017.1165771
TAACGGC2350.016.85090836
GATATAC2900.016.6949861
ATACCGT5650.016.3538326
TATACCG953.347896E-416.2103775
CCCTTAT1653.101559E-816.0051121
CCGTCGT5300.015.773449
CATGGGT12950.015.6292014
GTATAGG2152.1464075E-1015.3537411
CGTCGTA5450.015.33930910
ATTTCGT2355.0931703E-1114.97858542
ACCGTCG5450.014.9356438
GACGGCC7700.014.85700611
AACGCAG46000.014.7781246
GTACCGT1201.181714E-414.6665326
CGCAATA6150.014.66653236
CGGATCG900.00436057814.66653126
Produced by FastQC (version 0.11.5)