FastQCFastQC Report
Thu 2 Feb 2017
SRR4062473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342546
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG88720.6608339677001757No Hit
TCCATGTACTCTGCGTTGATACCAC70730.5268348347095742No Hit
GAGTACATGGAAGCAGTGGTATCAA36440.2714245917830749No Hit
GTATCAACGCAGAGTACTTTTTTTT32900.2450567801773645No Hit
CATGTACTCTGCGTTGATACCACTG29650.22084904353370385No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26160.19485365864558832No Hit
CCCATGTACTCTGCGTTGATACCAC23170.17258254093342051No Hit
TATCAACGCAGAGTACTTTTTTTTT23080.17191217284174995No Hit
GTACATGGGAAGCAGTGGTATCAAC23030.17153974612415515No Hit
GCTTCCATGTACTCTGCGTTGATAC21800.1623780488713236No Hit
GCGTTGATACCACTGCTTCCATGTA19740.1470340681064187No Hit
CATGGAAGCAGTGGTATCAACGCAG19100.14226700612120555No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18620.13869170963229566No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15950.11880412291273446No Hit
GTATCAACGCAGAGTACATGGAAGC15860.11813375482106385No Hit
GAATAGGACCGCGGTTCTATTTTGT14710.10956794031638394No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14660.10919551359878917No Hit
GCGCAAGACGGACCAGAGCGAAAGC14100.10502433436172766No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13710.10211940596448836No Hit
ACGCAGAGTACATGGAAGCAGTGGT13480.10040624306355238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1058.913048E-1114.477649
GCGTTAT1550.014.0855721
CGAGCCG2500.013.30182815
CCGTCGT2150.013.2571419
CGCGTAA1153.765308E-1013.21871510
CGTCGTA2250.013.09019910
GCGTAAC1207.385097E-1012.66746211
TAATATA2800.012.5520194
TCTATAC853.9657752E-612.2875553
CGTTATT1700.012.2870972
GACGGAG701.0900446E-412.2150547
AGCCCCG1800.012.14191616
ACTATAC951.0461317E-611.9935933
GTCGTAG2300.011.97901311
AGACGAT802.863829E-511.8757477
ATATACG2650.011.8309336
TACCGTC2650.011.8309337
CGAACCT2350.011.72632916
CGGTCCA2600.011.69347810
GTCGCGT1302.6102498E-911.6930428
Produced by FastQC (version 0.11.5)