##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062473_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342546
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085343072043713	32.0	32.0	32.0	32.0	32.0
2	30.648259352007305	32.0	32.0	32.0	32.0	32.0
3	30.626873865029577	32.0	32.0	32.0	32.0	32.0
4	30.71523806260642	32.0	32.0	32.0	32.0	32.0
5	30.433377329342907	32.0	32.0	32.0	32.0	32.0
6	34.10409997124866	36.0	36.0	36.0	32.0	36.0
7	33.96149703622818	36.0	36.0	36.0	32.0	36.0
8	33.93616606060426	36.0	36.0	36.0	32.0	36.0
9	34.16087493463911	36.0	36.0	36.0	32.0	36.0
10	33.713179287711554	36.0	36.0	36.0	27.0	36.0
11	34.1705163175042	36.0	36.0	36.0	32.0	36.0
12	33.87548955492028	36.0	36.0	36.0	32.0	36.0
13	34.03167042321083	36.0	36.0	36.0	32.0	36.0
14	33.89844593779282	36.0	36.0	36.0	32.0	36.0
15	33.8159623580868	36.0	36.0	36.0	32.0	36.0
16	33.83118194832803	36.0	36.0	36.0	32.0	36.0
17	33.71403512430859	36.0	36.0	36.0	27.0	36.0
18	33.74649732672102	36.0	36.0	36.0	32.0	36.0
19	33.740036468024186	36.0	36.0	36.0	32.0	36.0
20	33.717471133205116	36.0	36.0	36.0	27.0	36.0
21	33.690094790048164	36.0	36.0	36.0	27.0	36.0
22	33.63289898446683	36.0	36.0	36.0	27.0	36.0
23	33.56485364374852	36.0	36.0	36.0	27.0	36.0
24	33.529559508575495	36.0	36.0	36.0	27.0	36.0
25	33.0229131813733	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	57.0
5	120.0
6	231.0
7	66.0
8	200.0
9	227.0
10	161.0
11	51.0
12	99.0
13	90.0
14	219.0
15	315.0
16	458.0
17	611.0
18	897.0
19	1412.0
20	2111.0
21	3286.0
22	4923.0
23	7554.0
24	10405.0
25	14585.0
26	19738.0
27	25003.0
28	33055.0
29	43411.0
30	55265.0
31	73806.0
32	105173.0
33	146464.0
34	301368.0
35	491174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.55756144617803	16.75209600464827	11.057444122061776	25.63289842711193
2	16.539702332136315	20.053511984703203	36.6768640368029	26.729921646357585
3	19.455959263240302	24.317211593827214	27.736125791729144	28.49070335120334
4	12.620852212029424	15.262876375767128	35.12212238013859	36.994149032064854
5	14.513494704496447	36.73926034325771	33.362641030875906	15.38460392136993
6	34.761173053220205	34.332314405924585	17.10464150994013	13.801871030915084
7	30.32388670002877	30.198816077612356	21.003367515328232	18.47392970703064
8	28.762299952212928	32.198421461091556	19.824403015729473	19.214875570966036
9	27.390153907175552	14.52638296888828	18.29618799594114	39.78727512799503
10	15.738500775563773	26.886036869108697	31.688491826750987	25.68697052857654
11	37.55524980331182	21.139933853112147	21.54248278279286	19.762333560783173
12	24.485372649868435	23.82590380496071	28.793773158230756	22.8949503869401
13	30.180858743444876	19.290033230519576	25.01707762339142	25.512030402644125
14	23.90445080842432	19.545596921424735	24.849203508143905	31.70074876200704
15	25.634019807502867	26.67465568785249	23.040893895408452	24.650430609236192
16	26.170023011956374	25.32948259636909	24.134143449645872	24.36635094202866
17	24.212559521811926	25.588729915466907	25.78330145164237	24.415409111078795
18	25.397468226035368	24.08720471897727	26.961386456881307	23.553940598106056
19	25.218120279778695	26.037124824914564	25.06507435257545	23.679680542731287
20	25.26045589663201	23.871183771117995	26.308170969440337	24.560189362809655
21	26.208056444335053	24.719456439860082	23.98444200806987	25.08804510773499
22	25.175156954954524	24.94285765438877	24.789334224535104	25.0926511661216
23	23.823869891725618	24.544107862647575	25.29537514134745	26.336647104279358
24	24.136976860746806	25.931044667470193	25.032146138883256	24.899832332899745
25	25.10376461374951	24.521340269620183	24.719807573977736	25.65508754265257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	248.0
2	563.5
3	879.0
4	879.0
5	879.0
6	2391.5
7	3904.0
8	3904.0
9	3904.0
10	3921.0
11	3938.0
12	3938.0
13	3938.0
14	3912.0
15	3886.0
16	3886.0
17	3886.0
18	6230.5
19	8575.0
20	8575.0
21	8575.0
22	13599.5
23	18624.0
24	18624.0
25	18624.0
26	27553.0
27	36482.0
28	36482.0
29	36482.0
30	45227.5
31	53973.0
32	53973.0
33	53973.0
34	66226.0
35	78479.0
36	78479.0
37	78479.0
38	90038.0
39	101597.0
40	101597.0
41	101597.0
42	119729.0
43	137861.0
44	137861.0
45	137861.0
46	170885.5
47	203910.0
48	203910.0
49	203910.0
50	192831.5
51	181753.0
52	181753.0
53	181753.0
54	165452.0
55	149151.0
56	149151.0
57	149151.0
58	137498.5
59	125846.0
60	125846.0
61	125846.0
62	111514.5
63	97183.0
64	97183.0
65	97183.0
66	80788.0
67	64393.0
68	64393.0
69	64393.0
70	48654.5
71	32916.0
72	32916.0
73	32916.0
74	25725.0
75	18534.0
76	18534.0
77	18534.0
78	15173.0
79	11812.0
80	11812.0
81	11812.0
82	8320.0
83	4828.0
84	4828.0
85	4828.0
86	3516.0
87	2204.0
88	2204.0
89	2204.0
90	1516.0
91	828.0
92	828.0
93	828.0
94	497.0
95	166.0
96	166.0
97	166.0
98	371.0
99	576.0
100	576.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00826787313060409
2	0.0032028697713150987
3	0.005437430076883772
4	0.015195010077866979
5	0.034561199392795484
6	0.045585030233600936
7	0.06733475054113602
8	0.08781822000884885
9	0.09556469573482025
10	0.11813375482106385
11	0.11850618153865865
12	0.13183905802855173
13	0.1199214030655188
14	0.12409258230258033
15	0.11463294367567292
16	0.11202595665250949
17	0.12297530214979598
18	0.13012589512761574
19	0.13198802871558962
20	0.12126213924886001
21	0.13079626321928634
22	0.15150318871755605
23	0.13988347512859894
24	0.13362670627300666
25	0.13191354337207067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1342546.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.548643821382534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18291702839483	41.60954903663206
2	12.86961081032229	13.52561189182881
3	3.7169637664602377	5.859642151821107
4	1.4926552975306417	3.137480463121499
5	0.724170215860307	1.9027081369648493
6	0.4215911015530373	1.3292424380264924
7	0.27610613719772337	1.0156302142350662
8	0.20287533128266208	0.8528658818974078
9	0.14164834925345063	0.6699085787525737
>10	0.7815817951387795	7.607995284220408
>50	0.08333488913220725	3.0394881243361436
>100	0.09020835283281567	10.016714489444139
>500	0.01108069698441014	3.926777059647281
>1k	0.004972107621209679	4.316080553165789
>5k	2.841204354976959E-4	1.1903056959065414
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8872	0.6608339677001757	No Hit
TCCATGTACTCTGCGTTGATACCAC	7073	0.5268348347095742	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3644	0.2714245917830749	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3290	0.2450567801773645	No Hit
CATGTACTCTGCGTTGATACCACTG	2965	0.22084904353370385	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2616	0.19485365864558832	No Hit
CCCATGTACTCTGCGTTGATACCAC	2317	0.17258254093342051	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2308	0.17191217284174995	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2303	0.17153974612415515	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2180	0.1623780488713236	No Hit
GCGTTGATACCACTGCTTCCATGTA	1974	0.1470340681064187	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1910	0.14226700612120555	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1862	0.13869170963229566	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1595	0.11880412291273446	No Hit
GTATCAACGCAGAGTACATGGAAGC	1586	0.11813375482106385	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1471	0.10956794031638394	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1466	0.10919551359878917	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1410	0.10502433436172766	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1371	0.10211940596448836	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1348	0.10040624306355238	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.448534351895577E-5
2	0.0	0.0	0.0	0.0	7.448534351895577E-5
3	0.0	0.0	0.0	0.0	7.448534351895577E-5
4	0.0	0.0	0.0	7.448534351895577E-5	7.448534351895577E-5
5	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
6	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
7	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
8	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
9	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
10	0.0	0.0	0.0	1.4897068703791154E-4	7.448534351895577E-5
11	0.0	0.0	0.0	2.2345603055686734E-4	7.448534351895577E-5
12	0.0	0.0	0.0	2.2345603055686734E-4	2.2345603055686734E-4
13	0.0	0.0	0.0	2.979413740758231E-4	3.724267175947789E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	105	8.913048E-11	14.47764	9
GCGTTAT	155	0.0	14.085572	1
CGAGCCG	250	0.0	13.301828	15
CCGTCGT	215	0.0	13.257141	9
CGCGTAA	115	3.765308E-10	13.218715	10
CGTCGTA	225	0.0	13.090199	10
GCGTAAC	120	7.385097E-10	12.667462	11
TAATATA	280	0.0	12.552019	4
TCTATAC	85	3.9657752E-6	12.287555	3
CGTTATT	170	0.0	12.287097	2
GACGGAG	70	1.0900446E-4	12.215054	7
AGCCCCG	180	0.0	12.141916	16
ACTATAC	95	1.0461317E-6	11.993593	3
GTCGTAG	230	0.0	11.979013	11
AGACGAT	80	2.863829E-5	11.875747	7
ATATACG	265	0.0	11.830933	6
TACCGTC	265	0.0	11.830933	7
CGAACCT	235	0.0	11.726329	16
CGGTCCA	260	0.0	11.693478	10
GTCGCGT	130	2.6102498E-9	11.693042	8
>>END_MODULE