FastQCFastQC Report
Thu 2 Feb 2017
SRR4062473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342546
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA35850.26702995651545647No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31890.23753376048194996No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15680.11679301863772265No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14810.11031279375157349No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14090.10494984901820868No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13810.10286425939967793No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13740.10234286199504523No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13730.10226837665152629No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13500.1005552137505903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG654.9328647E-420.3075035
GTAATAC1051.7931488E-618.8569663
TCGTATA951.5974529E-518.52614244
TAACGCA600.007412917418.3331624
GATTTCG1355.527545E-817.92575841
CGCAATA4500.017.59983636
TAGGACG2500.017.5998364
GATCGAA750.001291052117.59983411
TAGCGGC4400.017.49983830
TCCTACA4150.016.964962
TTAACGG1053.775147E-516.76174735
CTAGCGG4600.016.73897629
TAGACCG800.001988628916.4998465
CGCTTTA800.001988628916.49984620
CAATACG4550.016.43940738
TAATACC1351.0857693E-616.2961444
ATTTCGT1351.0857693E-616.29614442
CGAATTT953.3468966E-416.21037522
TGTAGGA4500.016.1343842
GTATTAG3550.016.1179281
Produced by FastQC (version 0.11.5)