##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342546
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23250599979442	32.0	32.0	32.0	32.0	32.0
2	31.41683338969391	32.0	32.0	32.0	32.0	32.0
3	31.50999146397963	32.0	32.0	32.0	32.0	32.0
4	31.6115283945578	32.0	32.0	32.0	32.0	32.0
5	31.55724124164088	32.0	32.0	32.0	32.0	32.0
6	35.15652945969821	36.0	36.0	36.0	36.0	36.0
7	35.17087757142027	36.0	36.0	36.0	36.0	36.0
8	35.116168831459035	36.0	36.0	36.0	36.0	36.0
9	35.23673602245286	36.0	36.0	36.0	36.0	36.0
10	35.115353216947504	36.0	36.0	36.0	36.0	36.0
11	35.24089379432809	36.0	36.0	36.0	36.0	36.0
12	35.148993777494404	36.0	36.0	36.0	36.0	36.0
13	35.18975886114889	36.0	36.0	36.0	36.0	36.0
14	35.14980566773876	36.0	36.0	36.0	36.0	36.0
15	35.1119306154128	36.0	36.0	36.0	36.0	36.0
16	35.11815833498442	36.0	36.0	36.0	36.0	36.0
17	35.08717094237367	36.0	36.0	36.0	36.0	36.0
18	35.097843202393065	36.0	36.0	36.0	36.0	36.0
19	35.08079648667532	36.0	36.0	36.0	36.0	36.0
20	35.062075340435264	36.0	36.0	36.0	36.0	36.0
21	35.04874693306598	36.0	36.0	36.0	36.0	36.0
22	35.024925775355186	36.0	36.0	36.0	36.0	36.0
23	34.96597658478741	36.0	36.0	36.0	36.0	36.0
24	34.944549386017314	36.0	36.0	36.0	32.0	36.0
25	34.9120134431148	36.0	36.0	36.0	32.0	36.0
26	34.85750357902076	36.0	36.0	36.0	32.0	36.0
27	34.84584662276004	36.0	36.0	36.0	32.0	36.0
28	34.80704646246758	36.0	36.0	36.0	32.0	36.0
29	34.78282159419491	36.0	36.0	36.0	32.0	36.0
30	34.767616901022386	36.0	36.0	36.0	32.0	36.0
31	34.7520531884941	36.0	36.0	36.0	32.0	36.0
32	34.70090037883246	36.0	36.0	36.0	32.0	36.0
33	34.671407907066126	36.0	36.0	36.0	32.0	36.0
34	34.643957823419086	36.0	36.0	36.0	32.0	36.0
35	34.59168102992374	36.0	36.0	36.0	32.0	36.0
36	34.55670792658129	36.0	36.0	36.0	32.0	36.0
37	34.52431946465894	36.0	36.0	36.0	32.0	36.0
38	34.466906161874526	36.0	36.0	36.0	32.0	36.0
39	34.40681138672343	36.0	36.0	36.0	32.0	36.0
40	34.342478395526115	36.0	36.0	36.0	32.0	36.0
41	34.33492185742612	36.0	36.0	36.0	32.0	36.0
42	34.235602355524506	36.0	36.0	36.0	32.0	36.0
43	34.256680218033495	36.0	36.0	36.0	32.0	36.0
44	34.22892027535742	36.0	36.0	36.0	32.0	36.0
45	34.10909346867817	36.0	36.0	36.0	32.0	36.0
46	34.165652424572414	36.0	36.0	36.0	32.0	36.0
47	34.10255291066377	36.0	36.0	36.0	32.0	36.0
48	34.08641119186978	36.0	36.0	36.0	32.0	36.0
49	34.093263098620085	36.0	36.0	36.0	32.0	36.0
50	33.554995508533786	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	40.0
22	108.0
23	359.0
24	1114.0
25	2523.0
26	5473.0
27	10366.0
28	16882.0
29	24956.0
30	34459.0
31	47771.0
32	70147.0
33	103672.0
34	222499.0
35	802167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.89777218226313	17.360961600780723	11.56990192536196	26.17136429159419
2	15.917216885562416	20.52765892784783	36.30163516315877	27.253489023430983
3	18.850299543924542	24.45772605827308	28.211801352807846	28.48017304499453
4	12.362211117260303	15.71525083367907	35.321326765697634	36.601211283362986
5	14.229754511204831	36.7585915119482	33.44391924001114	15.567734736835833
6	34.86081223711192	34.59534508867154	16.796607025941146	13.747235648275394
7	30.17788589739197	30.291773987632453	21.032128508073466	18.49821160690211
8	28.711046027473174	32.35129373593158	19.752023394356694	19.18563684223855
9	27.38468551543113	14.284203297317186	18.296728752683332	40.03438243456835
10	15.744041544945201	26.866714436600308	31.72122221510473	25.668021803349756
11	37.65636335738217	20.95041808623317	21.645440826608546	19.74777772977611
12	24.433799661240656	23.751737370637578	28.799013218169062	23.0154497499527
13	30.315311356184445	19.14742586101333	25.086961638558382	25.45030114424385
14	23.9600728764601	19.417286260582507	24.874305982811762	31.748334880145634
15	25.690069465031367	26.639683109554536	22.91846983269102	24.75177759272308
16	26.250869616385586	25.25105285033064	24.108000768688743	24.39007676459503
17	24.29034088962315	25.572308136927894	25.69774145541382	24.439609518035134
18	25.54631275203978	23.974820974476852	26.97010009340462	23.508766180078748
19	25.345574751256194	25.897734602762213	25.128152033524366	23.628538612457227
20	25.361291158738698	23.67397467200379	26.318800249674872	24.645933919582642
21	26.378611980520596	24.638112958513155	24.107702827314668	24.875572233651585
22	25.237943429871308	24.777996433641754	24.85017273151162	25.133887404975326
23	23.861156340266927	24.236041074197832	25.447694157220685	26.45510842831456
24	24.278721176034193	25.63003427815509	25.057390957181358	25.033853588629366
25	25.170981106047762	24.22903945190705	24.84868302464124	25.751296417403946
26	24.1526174894566	25.05582676496746	25.643069213270902	25.148486532305036
27	25.69967807434531	24.898141292737826	24.861345533039465	24.5408350998774
28	24.63997509210113	24.101892970520193	25.708392859537028	25.549739077841654
29	24.09705142319146	25.14625197199947	26.121265118662603	24.63543148614647
30	24.910356889074937	24.727346400048862	26.08439487362072	24.277901837255484
31	24.561765481406223	24.85017273151162	24.920635866480552	25.667425920601605
32	24.326317310542805	25.04055726954607	25.0383227092405	25.59480271067062
33	23.960221847147135	24.607797423700937	25.576777257539035	25.855203471612892
34	24.539494363694057	25.046665067714624	26.026743217737046	24.387097350854273
35	25.749955681220605	24.574353504460927	25.648953555408905	24.026737258909563
36	24.50649735651516	25.454621294167946	25.27228117323354	24.766600176083355
37	25.725822429920463	25.00927342526811	25.110126580392777	24.15477756441865
38	24.561095113314554	24.915496377777743	25.12636438527991	25.397044123627794
39	24.854865308153315	24.69382799546533	25.21611922422025	25.2351874721611
40	25.519870455090555	24.848087141893092	25.063200813975833	24.568841589040524
41	23.927671752029354	25.264758153538132	25.842243021840595	24.965327072591926
42	26.091098554537425	25.398459345154656	25.15690337612268	23.353538724185242
43	24.98655539549483	23.95873214027676	25.183792585133023	25.87091987909539
44	24.538079142167195	24.82313455181424	25.745858987327065	24.892927318691502
45	24.871103113040448	24.977617154272554	25.236602693687963	24.914677038999038
46	24.09760871896936	24.6582607348437	25.967566053701034	25.27656449248591
47	24.90439806159342	24.249150494657165	26.331984155477727	24.51446728827169
48	25.11332945016409	26.270980659135702	24.336149375887306	24.2795405148129
49	23.999028711120513	25.13835652558646	26.008196367200824	24.8544183960922
50	24.018320415091925	26.444457024191347	24.37376447436438	25.163458086352346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	27.5
2	27.0
3	137.5
4	248.0
5	273.5
6	299.0
7	272.5
8	246.0
9	347.5
10	449.0
11	630.0
12	811.0
13	1463.5
14	2116.0
15	2878.5
16	3641.0
17	4190.5
18	4740.0
19	5163.0
20	5586.0
21	6269.0
22	6952.0
23	7501.0
24	8050.0
25	8466.5
26	8883.0
27	10538.0
28	12193.0
29	14028.0
30	15863.0
31	18063.0
32	20263.0
33	23357.5
34	26452.0
35	29871.5
36	33291.0
37	39768.5
38	46246.0
39	50088.0
40	53930.0
41	60403.5
42	66877.0
43	70479.5
44	74082.0
45	85380.0
46	96678.0
47	104724.0
48	112770.0
49	111714.5
50	110659.0
51	102838.5
52	95018.0
53	92215.0
54	89412.0
55	90357.5
56	91303.0
57	88410.0
58	85517.0
59	79003.5
60	72490.0
61	63799.0
62	55108.0
63	47894.0
64	40680.0
65	35272.0
66	29864.0
67	25994.5
68	22125.0
69	20371.5
70	18618.0
71	14527.0
72	10436.0
73	9418.5
74	8401.0
75	6017.5
76	3634.0
77	3386.0
78	3138.0
79	2692.5
80	2247.0
81	1749.5
82	1252.0
83	1117.5
84	983.0
85	780.0
86	577.0
87	408.5
88	240.0
89	157.0
90	74.0
91	47.5
92	21.0
93	19.5
94	18.0
95	12.0
96	6.0
97	4.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014971554047310112
2	0.0037242671759477883
3	3.724267175947789E-4
4	2.2345603055686734E-4
5	0.0
6	5.213974046326905E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.4897068703791154E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1342546.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.89091141910018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.011214853143	44.160088104725084
2	13.245912171166054	14.806522076476458
3	3.6320905077226637	6.090025464998459
4	1.4649834841286895	3.2751704856752535
5	0.7081336238376937	1.9789116821399473
6	0.42543115357282435	1.4266640951558596
7	0.2766191731503949	1.082234839236142
8	0.20176323161365659	0.9021384724600217
9	0.13741349287212998	0.6912148822114833
>10	0.7306140254701968	7.569187175462335
>50	0.07498076507251514	2.9376000237930433
>100	0.07903522566587363	9.325024937912943
>500	0.009124589724311807	3.5388225701624867
>1k	0.002683702860091708	2.216395189590386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3585	0.26702995651545647	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3189	0.23753376048194996	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1568	0.11679301863772265	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1481	0.11031279375157349	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1409	0.10494984901820868	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1381	0.10286425939967793	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1374	0.10234286199504523	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1373	0.10226837665152629	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1350	0.1005552137505903	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.448534351895577E-5	0.0
5	0.0	0.0	0.0	1.4897068703791154E-4	0.0
6	0.0	0.0	0.0	1.4897068703791154E-4	0.0
7	0.0	0.0	0.0	1.4897068703791154E-4	0.0
8	0.0	0.0	0.0	1.4897068703791154E-4	0.0
9	0.0	0.0	0.0	1.4897068703791154E-4	0.0
10	0.0	0.0	0.0	1.4897068703791154E-4	0.0
11	0.0	0.0	0.0	2.2345603055686734E-4	0.0
12	0.0	0.0	0.0	2.2345603055686734E-4	0.0
13	0.0	0.0	0.0	2.979413740758231E-4	7.448534351895577E-5
14	0.0	0.0	0.0	3.724267175947789E-4	7.448534351895577E-5
15	0.0	0.0	0.0	5.213974046326905E-4	7.448534351895577E-5
16	0.0	0.0	0.0	5.213974046326905E-4	1.4897068703791154E-4
17	0.0	0.0	0.0	5.213974046326905E-4	1.4897068703791154E-4
18	0.0	0.0	0.0	5.213974046326905E-4	1.4897068703791154E-4
19	0.0	0.0	0.0	5.213974046326905E-4	1.4897068703791154E-4
20	0.0	0.0	0.0	7.448534351895577E-4	2.979413740758231E-4
21	0.0	0.0	0.0	0.001042794809265381	2.979413740758231E-4
22	0.0	0.0	0.0	0.0017131629009359828	3.724267175947789E-4
23	0.0	0.0	0.0	0.0028304430537203196	3.724267175947789E-4
24	0.0	0.0	0.0	0.00580985679447855	3.724267175947789E-4
25	0.0	0.0	0.0	0.007820961069490356	3.724267175947789E-4
26	0.0	0.0	0.0	0.010874860153767544	4.4691206111373467E-4
27	0.0	0.0	0.0	0.013630817863968907	4.4691206111373467E-4
28	0.0	0.0	0.0	0.02026001343715597	4.4691206111373467E-4
29	0.0	0.0	0.0	0.03068796152980978	4.4691206111373467E-4
30	0.0	0.0	0.0	0.049458268096586634	4.4691206111373467E-4
31	0.0	0.0	0.0	0.0889355001616332	4.4691206111373467E-4
32	0.0	0.0	0.0	0.1407772992508264	4.4691206111373467E-4
33	0.0	0.0	0.0	0.18852240444647705	4.4691206111373467E-4
34	0.0	0.0	0.0	0.24446089742921284	4.4691206111373467E-4
35	0.0	0.0	0.0	0.3144771203370313	4.4691206111373467E-4
36	0.0	0.0	0.0	0.40155048691069056	4.4691206111373467E-4
37	0.0	0.0	0.0	0.5388269750161261	4.4691206111373467E-4
38	0.0	0.0	0.0	0.702322304040234	4.4691206111373467E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	65	4.9328647E-4	20.307503	5
GTAATAC	105	1.7931488E-6	18.856966	3
TCGTATA	95	1.5974529E-5	18.526142	44
TAACGCA	60	0.0074129174	18.333162	4
GATTTCG	135	5.527545E-8	17.925758	41
CGCAATA	450	0.0	17.599836	36
TAGGACG	250	0.0	17.599836	4
GATCGAA	75	0.0012910521	17.599834	11
TAGCGGC	440	0.0	17.499838	30
TCCTACA	415	0.0	16.96496	2
TTAACGG	105	3.775147E-5	16.761747	35
CTAGCGG	460	0.0	16.738976	29
TAGACCG	80	0.0019886289	16.499846	5
CGCTTTA	80	0.0019886289	16.499846	20
CAATACG	455	0.0	16.439407	38
TAATACC	135	1.0857693E-6	16.296144	4
ATTTCGT	135	1.0857693E-6	16.296144	42
CGAATTT	95	3.3468966E-4	16.210375	22
TGTAGGA	450	0.0	16.134384	2
GTATTAG	355	0.0	16.117928	1
>>END_MODULE