FastQCFastQC Report
Thu 2 Feb 2017
SRR4062472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827079
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG164651.990740908667733No Hit
TCCATGTACTCTGCGTTGATACCAC139611.6879886927367278No Hit
GAGTACATGGAAGCAGTGGTATCAA63930.7729612286129861No Hit
CATGTACTCTGCGTTGATACCACTG55390.6697062795694244No Hit
CCCATGTACTCTGCGTTGATACCAC43460.5254637102380788No Hit
GTACATGGGAAGCAGTGGTATCAAC41980.5075694099354475No Hit
GCTTCCATGTACTCTGCGTTGATAC41540.5022494828184491No Hit
GTATCAACGCAGAGTACTTTTTTTT40950.4951159441842919No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39470.47722164388166066No Hit
CATGGAAGCAGTGGTATCAACGCAG36890.4460275257865331No Hit
GCGTTGATACCACTGCTTCCATGTA36060.4359922087249223No Hit
TATCAACGCAGAGTACTTTTTTTTT30830.3727576204933265No Hit
GTATCAACGCAGAGTACATGGAAGC29200.35304970867353663No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27450.3318909076400199No Hit
ACTCTGCGTTGATACCACTGCTTCC23620.2855833602352375No Hit
ACGCAGAGTACATGGAAGCAGTGGT22400.2708326532290144No Hit
CAGTGGTATCAACGCAGAGTACATG21880.264545466636198No Hit
GTACATGGGGTGGTATCAACGCAAA19470.2354067749271835No Hit
GTATCAACGCAGAGTACATGGGAAG19160.23165864445838913No Hit
TATCAACGCAGAGTACATGGAAGCA18950.2291195883343671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16720.20215723044594286No Hit
GGTATCAACGCAGAGTACTTTTTTT13920.1683031487923161No Hit
GAGTACATGGGAAGCAGTGGTATCA13290.16068598042025006No Hit
GGTATCAACGCAGAGTACATGGAAG13230.15996053581338662No Hit
ATACCACTGCTTCCATGTACTCTGC12780.1545197012619109No Hit
GTACATGGGTGGTATCAACGCAAAA11970.14472619906925457No Hit
TATCAACGCAGAGTACATGGGAAGC11720.1417035132073236No Hit
GCGTTGATACCACTGCTTCCCATGT11630.14061534629702846No Hit
AAGCAGTGGTATCAACGCAGAGTAC11600.14025262399359675No Hit
GTACTCTGCGTTGATACCACTGCTT11070.13384452996630308No Hit
GCTTCCCATGTACTCTGCGTTGATA10590.12804097311139565No Hit
GGTATCAACGCAGAGTACATGGGAA10570.12779915824244117No Hit
AAAAAGTACTCTGCGTTGATACCAC10400.12574373185632812No Hit
GTACATGGGAGTGGTATCAACGCAA10380.12550191698737365No Hit
CCACTGCTTCCATGTACTCTGCGTT10250.12393012033916953No Hit
GTGGTATCAACGCAGAGTACATGGA9060.10954213563637814No Hit
ACATGGAAGCAGTGGTATCAACGCA9010.10893759846399195No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8600.10398039365042518No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8580.10373857878147069No Hit
ACGCAGAGTACATGGGAAGCAGTGG8530.10313404160908451No Hit
GAAGCAGTGGTATCAACGCAGAGTA8480.10252950443669831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG456.7531824E-414.7790496
TTATACT456.7624863E-414.7763654
CAAGACG1350.014.7763654
GCGTTAT400.00529453714.2426051
GCGCAAG1350.014.0667711
AGACGAT954.8821676E-914.00120457
AAGACGA1101.8553692E-1013.819376
GTTTTCG551.9569753E-413.8193715
TTCGTTC707.2372004E-613.57423918
AGGACCT3150.013.5709525
GGACCTG3300.013.5314676
AAGACGG1500.013.2995345
TTTACAC500.001501643413.2963153
CGTTATT500.001503013513.2947052
TCGTTCT655.435886E-513.1565719
CGCAAGA1550.012.8658452
CGCCACT751.4734587E-512.66928917
AGCCCCG1051.9841536E-812.66852316
CGGTTCT1051.9876097E-812.66698912
CGATCAG1051.9876097E-812.66698910
Produced by FastQC (version 0.11.5)