##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062472_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827079
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.911819789887062	32.0	32.0	32.0	32.0	32.0
2	30.69624787958587	32.0	32.0	32.0	32.0	32.0
3	30.70678012620318	32.0	32.0	32.0	32.0	32.0
4	30.708172979848356	32.0	32.0	32.0	32.0	32.0
5	30.60452145441971	32.0	32.0	32.0	32.0	32.0
6	34.17897443895928	36.0	36.0	36.0	32.0	36.0
7	33.96209551929139	36.0	36.0	36.0	32.0	36.0
8	33.95514817810632	36.0	36.0	36.0	32.0	36.0
9	34.06606140404968	36.0	36.0	36.0	32.0	36.0
10	33.833515299022224	36.0	36.0	36.0	32.0	36.0
11	34.1669767942361	36.0	36.0	36.0	32.0	36.0
12	33.94745604712488	36.0	36.0	36.0	32.0	36.0
13	34.03282274123754	36.0	36.0	36.0	32.0	36.0
14	33.94271889384206	36.0	36.0	36.0	32.0	36.0
15	33.92719558832953	36.0	36.0	36.0	32.0	36.0
16	33.89596036170668	36.0	36.0	36.0	32.0	36.0
17	33.80670528450124	36.0	36.0	36.0	32.0	36.0
18	33.850186016087946	36.0	36.0	36.0	32.0	36.0
19	33.82992072099521	36.0	36.0	36.0	32.0	36.0
20	33.77507710871634	36.0	36.0	36.0	32.0	36.0
21	33.663737079529284	36.0	36.0	36.0	27.0	36.0
22	33.623196816749065	36.0	36.0	36.0	27.0	36.0
23	33.54109825059033	36.0	36.0	36.0	27.0	36.0
24	33.537238885281816	36.0	36.0	36.0	27.0	36.0
25	32.96393573044413	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	9.0
4	38.0
5	90.0
6	133.0
7	34.0
8	125.0
9	154.0
10	109.0
11	40.0
12	61.0
13	45.0
14	246.0
15	324.0
16	444.0
17	656.0
18	862.0
19	1241.0
20	1800.0
21	2611.0
22	3762.0
23	5155.0
24	6543.0
25	8561.0
26	11475.0
27	14088.0
28	18631.0
29	24699.0
30	31421.0
31	42784.0
32	61425.0
33	87117.0
34	183297.0
35	319099.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.08510527671337	16.642583955957054	12.131156659343103	24.14115410798647
2	13.859896886275042	21.538894961356537	38.69533569998356	25.905872452384866
3	19.46805281988593	27.275079591360484	27.856311144483257	25.40055644427033
4	12.018265083144582	16.61875871443031	35.11953205398745	36.24344414843766
5	12.832828159433365	38.49376737165237	33.88164658469384	14.791757884220432
6	32.54922169480372	34.287767277794714	18.902872737302037	14.260138290099533
7	27.910606495076244	31.483370296296563	22.28679145750227	18.31923175112492
8	28.677943292537556	32.76369308892173	21.073179468747238	17.48518414979348
9	27.455494112478352	15.422051004612467	18.38148526031814	38.740969622591045
10	15.47966215979014	28.397195882678368	32.37772540132749	23.745416556204006
11	36.088749216156735	21.918194606189882	21.26170317338492	20.731353004268467
12	23.245939548764763	24.547100023609033	30.50468851208601	21.70227191554019
13	31.47546222821054	20.593957046339643	24.923098154718886	23.007482570730932
14	24.415991942044705	19.927701158331075	25.3797747259133	30.27653217371092
15	25.703820002203127	26.62792231923777	26.072177789829066	21.596079888730042
16	24.930184250632184	25.61956559896284	27.103278888952104	22.346971261452868
17	23.052701539534326	25.710477477183	28.50311550805117	22.7337054752315
18	25.066860377130055	23.630981567238717	30.36411513665668	20.938042918974546
19	23.670561171983774	28.836612385737638	25.84236333918518	21.650463103093408
20	23.448558056651148	23.69491476179062	30.557553042353984	22.29897413920425
21	24.848481913474274	26.36959516885519	23.97484666587568	24.80707625179485
22	23.019206083944876	27.222504783356342	25.511153091622468	24.247136041076317
23	21.83115304328453	26.522489841188353	25.632050021552665	26.01430709397446
24	22.036375171318294	28.807382547085286	25.7605805692368	23.395661712359615
25	24.845207798088495	25.336281064379513	24.788666571422343	25.02984456610965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	282.0
2	700.5
3	1119.0
4	1119.0
5	1119.0
6	3085.0
7	5051.0
8	5051.0
9	5051.0
10	4721.5
11	4392.0
12	4392.0
13	4392.0
14	3537.0
15	2682.0
16	2682.0
17	2682.0
18	4492.0
19	6302.0
20	6302.0
21	6302.0
22	10187.5
23	14073.0
24	14073.0
25	14073.0
26	21443.5
27	28814.0
28	28814.0
29	28814.0
30	36552.0
31	44290.0
32	44290.0
33	44290.0
34	48871.5
35	53453.0
36	53453.0
37	53453.0
38	58361.0
39	63269.0
40	63269.0
41	63269.0
42	77329.0
43	91389.0
44	91389.0
45	91389.0
46	128976.5
47	166564.0
48	166564.0
49	166564.0
50	138908.0
51	111252.0
52	111252.0
53	111252.0
54	90957.0
55	70662.0
56	70662.0
57	70662.0
58	63398.5
59	56135.0
60	56135.0
61	56135.0
62	49931.0
63	43727.0
64	43727.0
65	43727.0
66	36634.5
67	29542.0
68	29542.0
69	29542.0
70	22360.0
71	15178.0
72	15178.0
73	15178.0
74	11960.0
75	8742.0
76	8742.0
77	8742.0
78	7285.5
79	5829.0
80	5829.0
81	5829.0
82	4092.5
83	2356.0
84	2356.0
85	2356.0
86	1714.0
87	1072.0
88	1072.0
89	1072.0
90	748.5
91	425.0
92	425.0
93	425.0
94	256.5
95	88.0
96	88.0
97	88.0
98	239.5
99	391.0
100	391.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008221705544452222
2	0.003748130468794396
3	0.00507811224804402
4	0.01765248543367683
5	0.03639313777764881
6	0.04969295557014506
7	0.06964268225888941
8	0.09285690967851923
9	0.1015622449608804
10	0.12187469395305649
11	0.12344649060126059
12	0.13589995635241617
13	0.12405102777364677
14	0.1285246028493046
15	0.1184892857876938
16	0.11800565604978486
17	0.1269528062011005
18	0.13348180766287138
19	0.13650449352480234
20	0.12586463929080535
21	0.1336027150973486
22	0.1570587573859329
23	0.14448438420030008
24	0.13674630839375682
25	0.1369881232627113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	827079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.52280649454612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30138477952687	34.416745962993275
2	13.6684382005854	12.171144581746518
3	4.149454698464903	5.542361057929143
4	1.7022456401473813	3.031550129698666
5	0.814849736599022	1.8139698572365068
6	0.45239315982461187	1.208508786859648
7	0.3073161642572028	0.9577804679708714
8	0.22117937851583927	0.7878021336195745
9	0.15658196667638327	0.6274321742581273
>10	0.9630453234625651	8.332568224329014
>50	0.12433021480591144	3.8797259058211986
>100	0.11724109423890036	10.333464763642688
>500	0.011996763131396922	3.5800033990912548
>1k	0.008452264933484195	8.174974361839286
>5k	5.453074150634965E-4	1.4484622616376812
>10k+	5.453074150634965E-4	3.693505931326524
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	16465	1.990740908667733	No Hit
TCCATGTACTCTGCGTTGATACCAC	13961	1.6879886927367278	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6393	0.7729612286129861	No Hit
CATGTACTCTGCGTTGATACCACTG	5539	0.6697062795694244	No Hit
CCCATGTACTCTGCGTTGATACCAC	4346	0.5254637102380788	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4198	0.5075694099354475	No Hit
GCTTCCATGTACTCTGCGTTGATAC	4154	0.5022494828184491	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4095	0.4951159441842919	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3947	0.47722164388166066	No Hit
CATGGAAGCAGTGGTATCAACGCAG	3689	0.4460275257865331	No Hit
GCGTTGATACCACTGCTTCCATGTA	3606	0.4359922087249223	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3083	0.3727576204933265	No Hit
GTATCAACGCAGAGTACATGGAAGC	2920	0.35304970867353663	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2745	0.3318909076400199	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2362	0.2855833602352375	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2240	0.2708326532290144	No Hit
CAGTGGTATCAACGCAGAGTACATG	2188	0.264545466636198	No Hit
GTACATGGGGTGGTATCAACGCAAA	1947	0.2354067749271835	No Hit
GTATCAACGCAGAGTACATGGGAAG	1916	0.23165864445838913	No Hit
TATCAACGCAGAGTACATGGAAGCA	1895	0.2291195883343671	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1672	0.20215723044594286	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1392	0.1683031487923161	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1329	0.16068598042025006	No Hit
GGTATCAACGCAGAGTACATGGAAG	1323	0.15996053581338662	No Hit
ATACCACTGCTTCCATGTACTCTGC	1278	0.1545197012619109	No Hit
GTACATGGGTGGTATCAACGCAAAA	1197	0.14472619906925457	No Hit
TATCAACGCAGAGTACATGGGAAGC	1172	0.1417035132073236	No Hit
GCGTTGATACCACTGCTTCCCATGT	1163	0.14061534629702846	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1160	0.14025262399359675	No Hit
GTACTCTGCGTTGATACCACTGCTT	1107	0.13384452996630308	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1059	0.12804097311139565	No Hit
GGTATCAACGCAGAGTACATGGGAA	1057	0.12779915824244117	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1040	0.12574373185632812	No Hit
GTACATGGGAGTGGTATCAACGCAA	1038	0.12550191698737365	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1025	0.12393012033916953	No Hit
GTGGTATCAACGCAGAGTACATGGA	906	0.10954213563637814	No Hit
ACATGGAAGCAGTGGTATCAACGCA	901	0.10893759846399195	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	860	0.10398039365042518	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	858	0.10373857878147069	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	853	0.10313404160908451	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	848	0.10252950443669831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.2090743447723857E-4
7	0.0	0.0	0.0	1.2090743447723857E-4	1.2090743447723857E-4
8	0.0	0.0	0.0	1.2090743447723857E-4	1.2090743447723857E-4
9	0.0	0.0	0.0	2.4181486895447714E-4	1.2090743447723857E-4
10	0.0	0.0	0.0	2.4181486895447714E-4	1.2090743447723857E-4
11	0.0	0.0	0.0	2.4181486895447714E-4	1.2090743447723857E-4
12	0.0	0.0	0.0	2.4181486895447714E-4	2.4181486895447714E-4
13	0.0	0.0	0.0	2.4181486895447714E-4	2.4181486895447714E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGG	45	6.7531824E-4	14.779049	6
TTATACT	45	6.7624863E-4	14.776365	4
CAAGACG	135	0.0	14.776365	4
GCGTTAT	40	0.005294537	14.242605	1
GCGCAAG	135	0.0	14.066771	1
AGACGAT	95	4.8821676E-9	14.0012045	7
AAGACGA	110	1.8553692E-10	13.81937	6
GTTTTCG	55	1.9569753E-4	13.81937	15
TTCGTTC	70	7.2372004E-6	13.574239	18
AGGACCT	315	0.0	13.570952	5
GGACCTG	330	0.0	13.531467	6
AAGACGG	150	0.0	13.299534	5
TTTACAC	50	0.0015016434	13.296315	3
CGTTATT	50	0.0015030135	13.294705	2
TCGTTCT	65	5.435886E-5	13.15657	19
CGCAAGA	155	0.0	12.865845	2
CGCCACT	75	1.4734587E-5	12.669289	17
AGCCCCG	105	1.9841536E-8	12.668523	16
CGGTTCT	105	1.9876097E-8	12.666989	12
CGATCAG	105	1.9876097E-8	12.666989	10
>>END_MODULE