FastQCFastQC Report
Thu 2 Feb 2017
SRR4062472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827079
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73340.8867351244560676No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65570.7927900478672534No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA26160.31629384859245613No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC24140.2918705468280539No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16610.20082724866669327No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG16530.19985998919087536No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16380.19804637767371677No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG16130.1950236918117858No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC16100.1946609695083541No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15590.18849469035001493No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA15050.18196568888824405No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12520.1513761079655027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10200.12332558316678335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA9970.12054471217380686No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC9380.11341117353964977No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG8880.10736580181578785No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8610.10410130108490241No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8410.10168315239535765No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA8280.10011135574715353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC6350.024.9446413
TCTAGGT450.001397919124.444213
CGATCAT450.001397919124.4442143
CTATACT651.8126275E-523.6920814
CTATAGC752.0566295E-623.4749551
TGTAGGA7050.023.0933742
CTATTCG500.00257989921.99978838
ATAACGC2000.021.9997883
TAACGCC1900.021.9997864
CTGTAGG6850.021.525851
AACGCCG2050.021.463215
GTCGTAT1451.2732926E-1121.24117543
CCTATAC856.1100945E-620.7056833
AACCTAT755.4703632E-520.5331366
ACGCCGC2150.020.464926
AATAACG2050.020.3912812
TATTAGC1301.6207196E-920.3087252
ATACACT4550.020.3074974
TAGGACC6350.020.0942964
GATATAC4400.020.0070651
Produced by FastQC (version 0.11.5)