##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827079
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085734252713465	32.0	32.0	32.0	32.0	32.0
2	31.39522101274485	32.0	32.0	32.0	32.0	32.0
3	31.488463617139356	32.0	32.0	32.0	32.0	32.0
4	31.608778605187656	32.0	32.0	32.0	32.0	32.0
5	31.54113331374633	32.0	32.0	32.0	32.0	32.0
6	35.12196416545457	36.0	36.0	36.0	36.0	36.0
7	35.13154607963689	36.0	36.0	36.0	36.0	36.0
8	35.08875814764974	36.0	36.0	36.0	36.0	36.0
9	35.1656129583752	36.0	36.0	36.0	36.0	36.0
10	35.083071871006275	36.0	36.0	36.0	36.0	36.0
11	35.18823715751458	36.0	36.0	36.0	36.0	36.0
12	35.0867909836908	36.0	36.0	36.0	36.0	36.0
13	35.139165666157645	36.0	36.0	36.0	36.0	36.0
14	35.07408723955027	36.0	36.0	36.0	36.0	36.0
15	35.05838861825775	36.0	36.0	36.0	36.0	36.0
16	35.054060132103466	36.0	36.0	36.0	36.0	36.0
17	35.036977120686174	36.0	36.0	36.0	36.0	36.0
18	35.042920930165074	36.0	36.0	36.0	36.0	36.0
19	34.99335250925244	36.0	36.0	36.0	36.0	36.0
20	34.961362820238456	36.0	36.0	36.0	36.0	36.0
21	34.887797900805126	36.0	36.0	36.0	32.0	36.0
22	34.86319444696335	36.0	36.0	36.0	32.0	36.0
23	34.77276052227175	36.0	36.0	36.0	32.0	36.0
24	34.754185513113015	36.0	36.0	36.0	32.0	36.0
25	34.7191598384193	36.0	36.0	36.0	32.0	36.0
26	34.648600677807075	36.0	36.0	36.0	32.0	36.0
27	34.610684106355016	36.0	36.0	36.0	32.0	36.0
28	34.533576599091504	36.0	36.0	36.0	32.0	36.0
29	34.49609045810618	36.0	36.0	36.0	32.0	36.0
30	34.45741337889125	36.0	36.0	36.0	32.0	36.0
31	34.41735674584895	36.0	36.0	36.0	32.0	36.0
32	34.348651096207256	36.0	36.0	36.0	32.0	36.0
33	34.29041119409391	36.0	36.0	36.0	32.0	36.0
34	34.23217491920361	36.0	36.0	36.0	32.0	36.0
35	34.106101110051156	36.0	36.0	36.0	32.0	36.0
36	34.043512167519665	36.0	36.0	36.0	32.0	36.0
37	33.92717987036305	36.0	36.0	36.0	32.0	36.0
38	33.81519903177327	36.0	36.0	36.0	32.0	36.0
39	33.607139100376145	36.0	36.0	36.0	27.0	36.0
40	33.452298994412864	36.0	36.0	36.0	21.0	36.0
41	33.45877600567781	36.0	36.0	36.0	21.0	36.0
42	33.217160633990225	36.0	36.0	36.0	14.0	36.0
43	33.269466399219425	36.0	36.0	36.0	14.0	36.0
44	33.25195416640974	36.0	36.0	36.0	14.0	36.0
45	32.947862296104724	36.0	36.0	36.0	14.0	36.0
46	33.12754887985307	36.0	36.0	36.0	14.0	36.0
47	32.93423965546217	36.0	36.0	36.0	14.0	36.0
48	32.92573019022367	36.0	36.0	36.0	14.0	36.0
49	33.00358490543225	36.0	36.0	36.0	14.0	36.0
50	32.35255640634087	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	22.0
22	119.0
23	363.0
24	1047.0
25	2636.0
26	5518.0
27	10384.0
28	17089.0
29	23865.0
30	31370.0
31	41042.0
32	57633.0
33	71855.0
34	134659.0
35	429471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.334212069726526	17.3885808608795	12.787549353342726	24.489657716051248
2	13.447489523054118	22.102668024095163	38.073692890669776	26.376149562180945
3	19.06112618429262	27.229057547311253	28.443939855587637	25.265876412808495
4	11.81120545921248	17.0391220185738	35.20147410344114	35.94819841877257
5	12.646313109146767	38.40733472860512	33.98030901522104	14.966043147027067
6	32.91031494235085	34.53024645980036	18.414237212721503	14.145201385127294
7	27.635449576158987	31.43411935256487	22.376822528440453	18.55360854283569
8	28.612537148854162	32.82084640142768	21.150363085464733	17.416253364253432
9	27.379609444805155	14.943675271648779	18.439351017254697	39.23736426629137
10	15.405420763917352	28.308420356459298	32.561702086499594	23.724456793123753
11	36.31817516827292	21.593705075331375	21.399285920691977	20.688833835703726
12	23.145672904281213	24.56693979656115	30.666961680806793	21.620425618350847
13	31.76322938921191	20.243410847089578	25.099295230564433	22.894064533134078
14	24.26829843340237	19.749503977250058	25.573252373715206	30.408945215632365
15	25.647852260787662	26.639655945804453	26.026050715832465	21.68644107757542
16	25.008735562140984	25.20593558777336	27.230893300398147	22.554435549687515
17	23.036614398382742	25.69343436358558	28.74574254696347	22.524208691068203
18	25.274127380818516	23.00094670521196	30.70758657879114	21.01733933517838
19	23.80074938427889	28.357992404594967	26.280802680276004	21.560455530850138
20	23.679962857236127	23.295356308164035	30.891486786631024	22.133194047968814
21	24.74866367058044	26.054463962934616	24.48907540875781	24.707796957727133
22	22.95971727005522	26.742185450241152	26.047572239169416	24.250525040534217
23	21.91011983135831	25.68654263982038	26.1209630518971	26.282374476924215
24	22.239834405177742	28.15208704368023	26.199431976872827	23.408646574269206
25	25.199527493746064	24.322706778917127	25.27461101055643	25.20315471678038
26	21.536878581127077	25.40893917026064	27.84933482774922	25.204847420863064
27	25.0394460504982	26.39276296460193	26.93479099336339	21.63299999153648
28	23.23417714631855	24.10942606449928	27.158711562015235	25.497685227166933
29	21.8894446600627	26.307523223295476	28.82808050984247	22.97495160679935
30	24.699937974486115	24.881299126201974	28.13491818798446	22.283844711327454
31	22.276590265258818	25.532627475730855	26.80505731616931	25.38572494284101
32	22.184216985318212	26.662144728617214	27.11736121942402	24.03627706664055
33	21.990885997589103	25.77698140080936	27.34382084419989	24.888311757401652
34	21.574964422987406	27.327740155414414	28.866045444268323	22.231249977329856
35	24.647464147922992	26.00876095270222	27.459287444125653	21.884487455249136
36	23.077602018670525	27.16125061813926	27.16294332222194	22.598204040968277
37	24.613610066269366	26.716553074131976	26.91834758227449	21.751489277324172
38	23.274681136868423	26.765036955357346	25.26106937789498	24.69921252987925
39	22.9587500105794	25.782059513057398	27.71367668626576	23.545513790097438
40	25.008614654706502	26.082151765429906	25.558622574143463	23.350611005720133
41	21.729484124249314	26.150706280778497	28.174213104189565	23.945596490782624
42	25.76150524919627	26.92620656551551	26.54595268408459	20.766335501203635
43	23.985737759029067	24.568390685774876	25.986997614496314	25.45887394069974
44	22.99949581599823	25.98784396653766	28.356178793077806	22.656481424386303
45	24.132761199353386	26.178273175839312	26.432299695675987	23.25666592913132
46	22.311411606388265	25.42332715496343	28.470073596355366	23.795187642292937
47	24.338182930530216	24.650245018915967	28.507796715912264	22.503775334641553
48	22.999979445736138	29.285594241904338	24.7706688236553	22.94375748870422
49	21.92317783428185	26.128942942572596	29.377483892107044	22.570395331038508
50	21.850875188464464	29.720256468850014	23.844155153256217	24.584713189429305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	29.0
2	36.0
3	131.5
4	227.0
5	264.5
6	302.0
7	306.5
8	311.0
9	413.0
10	515.0
11	758.0
12	1001.0
13	1814.0
14	2627.0
15	3557.5
16	4488.0
17	5326.0
18	6164.0
19	6637.5
20	7111.0
21	7536.0
22	7961.0
23	8295.0
24	8629.0
25	8217.5
26	7806.0
27	9275.5
28	10745.0
29	12513.0
30	14281.0
31	16163.0
32	18045.0
33	21007.0
34	23969.0
35	26769.0
36	29569.0
37	32020.5
38	34472.0
39	34875.0
40	35278.0
41	37660.0
42	40042.0
43	43505.0
44	46968.0
45	57697.5
46	68427.0
47	77357.0
48	86287.0
49	80446.5
50	74606.0
51	62933.5
52	51261.0
53	46600.5
54	41940.0
55	41981.0
56	42022.0
57	40320.5
58	38619.0
59	35289.5
60	31960.0
61	28167.5
62	24375.0
63	21517.5
64	18660.0
65	16300.0
66	13940.0
67	12161.0
68	10382.0
69	9580.0
70	8778.0
71	6876.0
72	4974.0
73	4553.5
74	4133.0
75	3039.0
76	1945.0
77	1741.5
78	1538.0
79	1297.0
80	1056.0
81	834.0
82	612.0
83	543.0
84	474.0
85	400.0
86	326.0
87	225.0
88	124.0
89	84.5
90	45.0
91	28.5
92	12.0
93	8.5
94	5.0
95	3.0
96	1.0
97	3.5
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016201596219949967
2	0.0030226858619309643
3	3.6272230343171574E-4
4	0.0
5	0.0
6	8.463520413406699E-4
7	0.0
8	1.2090743447723857E-4
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	827079.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.506270678228475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50166475689078	41.218296584168556
2	13.004506386251165	13.656362647065093
3	3.9141235921368227	6.165480983903279
4	1.5891759785924133	3.337668163492475
5	0.78539543856527	2.061909274337702
6	0.4657794492678835	1.4673805103769408
7	0.29875094773167954	1.0980408688883798
8	0.20624667957716028	0.8663395187491387
9	0.15118666408886597	0.7144423116829561
>10	0.8684530067095781	8.65345225942933
>50	0.10909619636172076	3.9836090306401206
>100	0.09357641479542946	9.62250128048119
>500	0.009033366773390731	3.210216551588612
>1k	0.0025478726796743087	2.254910785081242
>5k	4.6324957812260165E-4	1.6893892301150708
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	7334	0.8867351244560676	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6557	0.7927900478672534	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2616	0.31629384859245613	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2414	0.2918705468280539	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1661	0.20082724866669327	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1653	0.19985998919087536	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1638	0.19804637767371677	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1613	0.1950236918117858	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1610	0.1946609695083541	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1559	0.18849469035001493	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1505	0.18196568888824405	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1252	0.1513761079655027	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1020	0.12332558316678335	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	997	0.12054471217380686	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	938	0.11341117353964977	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	888	0.10736580181578785	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	861	0.10410130108490241	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	841	0.10168315239535765	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	828	0.10011135574715353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2090743447723857E-4	0.0
4	0.0	0.0	0.0	1.2090743447723857E-4	0.0
5	0.0	0.0	0.0	1.2090743447723857E-4	0.0
6	0.0	0.0	0.0	1.2090743447723857E-4	0.0
7	0.0	0.0	0.0	1.2090743447723857E-4	0.0
8	0.0	0.0	0.0	2.4181486895447714E-4	0.0
9	0.0	0.0	0.0	2.4181486895447714E-4	0.0
10	0.0	0.0	0.0	2.4181486895447714E-4	1.2090743447723857E-4
11	0.0	0.0	0.0	2.4181486895447714E-4	1.2090743447723857E-4
12	0.0	0.0	0.0	2.4181486895447714E-4	4.836297379089543E-4
13	0.0	0.0	0.0	3.6272230343171574E-4	8.4635204134067E-4
14	0.0	0.0	0.0	3.6272230343171574E-4	8.4635204134067E-4
15	0.0	0.0	0.0	4.836297379089543E-4	9.672594758179086E-4
16	0.0	0.0	0.0	4.836297379089543E-4	0.0010881669102951472
17	0.0	0.0	0.0	6.045371723861928E-4	0.0012090743447723856
18	0.0	0.0	0.0	7.254446068634315E-4	0.0013299817792496243
19	0.0	0.0	0.0	8.4635204134067E-4	0.0013299817792496243
20	0.0	0.0	0.0	0.0010881669102951472	0.001450889213726863
21	0.0	0.0	0.0	0.002297241255067533	0.001450889213726863
22	0.0	0.0	0.0	0.004473575075657827	0.00169270408268134
23	0.0	0.0	0.0	0.008705335282361178	0.00169270408268134
24	0.0	0.0	0.0	0.014508892137268628	0.00169270408268134
25	0.0	0.0	0.0	0.018136115171585785	0.00169270408268134
26	0.0	0.0	0.0	0.02357694972306152	0.0019345189516358171
27	0.0	0.0	0.0	0.031919562701990985	0.0019345189516358171
28	0.0	0.0	0.0	0.0454611953634417	0.0019345189516358171
29	0.0	0.0	0.0	0.06311368079711853	0.0019345189516358171
30	0.0	0.0	0.0	0.09926500370581287	0.0019345189516358171
31	0.0	0.0	0.0	0.1635877588477038	0.0019345189516358171
32	0.0	0.0	0.0	0.24846477785072527	0.0019345189516358171
33	0.0	0.0	0.0	0.31641475602693336	0.0019345189516358171
34	0.0	0.0	0.0	0.41241525900186077	0.0021763338205902944
35	0.0	0.0	0.0	0.5067230578941069	0.0021763338205902944
36	0.0	0.0	0.0	0.6455247926739768	0.0021763338205902944
37	0.0	0.0	0.0	0.8474402082509651	0.0021763338205902944
38	0.0	0.0	0.0	1.069426257951175	0.0021763338205902944
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGAC	635	0.0	24.944641	3
TCTAGGT	45	0.0013979191	24.44421	3
CGATCAT	45	0.0013979191	24.44421	43
CTATACT	65	1.8126275E-5	23.692081	4
CTATAGC	75	2.0566295E-6	23.474955	1
TGTAGGA	705	0.0	23.093374	2
CTATTCG	50	0.002579899	21.999788	38
ATAACGC	200	0.0	21.999788	3
TAACGCC	190	0.0	21.999786	4
CTGTAGG	685	0.0	21.52585	1
AACGCCG	205	0.0	21.46321	5
GTCGTAT	145	1.2732926E-11	21.241175	43
CCTATAC	85	6.1100945E-6	20.705683	3
AACCTAT	75	5.4703632E-5	20.533136	6
ACGCCGC	215	0.0	20.46492	6
AATAACG	205	0.0	20.391281	2
TATTAGC	130	1.6207196E-9	20.308725	2
ATACACT	455	0.0	20.307497	4
TAGGACC	635	0.0	20.094296	4
GATATAC	440	0.0	20.007065	1
>>END_MODULE