FastQCFastQC Report
Thu 2 Feb 2017
SRR4062471_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062471_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458405
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57870.23539652742326833No Hit
GTACTTTTTTTTTTTTTTTTTTTTT38480.15652425047947754No Hit
TATCAACGCAGAGTACTTTTTTTTT37390.15209048143003287No Hit
GTACATGGAAGCAGTGGTATCAACG33720.13716210307089355No Hit
TCCATGTACTCTGCGTTGATACCAC28530.11605085411069373No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27460.11169843862178933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA1500.013.93461716
CGAACGA2050.013.44026416
CGTCTTA1155.329639E-912.39118515
ACGGTAT3950.012.2669549
ATAATAC1405.2750693E-1112.2109353
AACCGCG1800.012.1402557
CGAACGT1103.8222424E-812.08980754
GTCTTAC1650.012.0851311
CGTCGTA3350.011.91154610
CGACCAT3050.011.52565310
CGCCAGT3500.011.40267818
ATCGCCA3750.011.40105116
CTAATCT1251.835906E-811.3989614
CGCTTAC1201.2721648E-711.08593618
ACCGAGT600.0058723811.0845818
CGCATCG3700.011.04065813
ACGAACG2500.011.01989415
TCGCCAG3800.011.00146317
CCGTCGT3650.010.9325149
AGGACGT9100.010.85703855
Produced by FastQC (version 0.11.5)