##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062471_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2458405
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125025372141693	32.0	32.0	32.0	32.0	32.0
2	30.80299950577712	32.0	32.0	32.0	32.0	32.0
3	30.7898360929139	32.0	32.0	32.0	32.0	32.0
4	30.84635729263486	32.0	32.0	32.0	32.0	32.0
5	30.682758129763	32.0	32.0	32.0	32.0	32.0
6	34.32443189791755	36.0	36.0	36.0	32.0	36.0
7	34.18512531499082	36.0	36.0	36.0	32.0	36.0
8	34.189727892678384	36.0	36.0	36.0	32.0	36.0
9	34.32132947988635	36.0	36.0	36.0	32.0	36.0
10	34.01238282544983	36.0	36.0	36.0	32.0	36.0
11	34.32374201972417	36.0	36.0	36.0	32.0	36.0
12	34.100710826735224	36.0	36.0	36.0	32.0	36.0
13	34.21326998602753	36.0	36.0	36.0	32.0	36.0
14	34.12722598595431	36.0	36.0	36.0	32.0	36.0
15	34.07811975650879	36.0	36.0	36.0	32.0	36.0
16	34.07963252596704	36.0	36.0	36.0	32.0	36.0
17	34.006186938279086	36.0	36.0	36.0	32.0	36.0
18	34.02578379070983	36.0	36.0	36.0	32.0	36.0
19	34.00239748942912	36.0	36.0	36.0	32.0	36.0
20	33.98682723147732	36.0	36.0	36.0	32.0	36.0
21	33.95449081823377	36.0	36.0	36.0	32.0	36.0
22	33.91359316304677	36.0	36.0	36.0	32.0	36.0
23	33.88930587108308	36.0	36.0	36.0	32.0	36.0
24	33.85966754867485	36.0	36.0	36.0	32.0	36.0
25	33.417928290904065	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	102.0
5	240.0
6	412.0
7	113.0
8	373.0
9	436.0
10	315.0
11	90.0
12	170.0
13	145.0
14	469.0
15	631.0
16	890.0
17	1286.0
18	1873.0
19	2638.0
20	4002.0
21	5644.0
22	8377.0
23	12144.0
24	16337.0
25	22914.0
26	30940.0
27	39283.0
28	51881.0
29	68437.0
30	86957.0
31	117278.0
32	164890.0
33	236624.0
34	532112.0
35	1050386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14814002711761	17.399166294101207	11.204992634797845	26.247701043983334
2	16.58040888750041	20.138598210410404	37.5392797302558	25.741713171833386
3	19.164026273622685	24.17667127828651	28.136182856082886	28.523119592007916
4	12.680148101317833	15.614103391839132	35.803343060568004	35.90240544627503
5	14.558933755221723	36.61855316046001	33.52584432611387	15.296668758204401
6	33.66654946314275	35.34817585737633	17.2994029131587	13.685871766322219
7	29.955714029860463	30.396090786238787	21.026962340643774	18.621232843256973
8	27.77164582049999	33.18394066852946	19.711688985317366	19.332724525653184
9	27.75411022181904	14.306409865428126	18.718808669830878	39.22067124292196
10	16.0115252649691	26.878669096610636	31.692116596585183	25.417689041835086
11	37.09133856664325	21.197348727816976	22.5699420670556	19.141370638484172
12	24.861167116748334	23.697023758609596	29.102199086802628	22.339610037839446
13	29.90213367326575	19.828418296075164	25.0648573139094	25.20459071674968
14	23.414406070721363	20.096801809617133	25.17307274064625	31.31571937901525
15	24.958013924227963	27.743185305606506	22.46316139268343	24.835639377482106
16	25.25821580949022	25.783178944787856	24.560749079349616	24.397856166372307
17	23.779106303655727	25.929265567924446	25.716424481530765	24.57520364688906
18	24.45448854316987	24.96479881458455	27.052105900480335	23.528606741765245
19	25.479442346355246	25.003502849527358	25.516670305285555	24.00038449883184
20	25.62880645899634	24.595640731361435	25.545845303714394	24.229707505927834
21	26.341110751328312	24.311854300185118	24.42752786449901	24.91950708398756
22	25.54001252332847	25.163375660336694	24.866425298590126	24.43018651774471
23	24.201682726089587	24.87652472865991	25.60786494198444	25.313927603266063
24	24.549773303174977	25.339821208688573	25.533707991027445	24.57669749710901
25	24.597842069462715	25.00625221475034	25.661207349508985	24.734698366277957
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	393.0
1	393.0
2	1020.0
3	1647.0
4	1647.0
5	1647.0
6	4014.0
7	6381.0
8	6381.0
9	6381.0
10	6689.5
11	6998.0
12	6998.0
13	6998.0
14	7582.0
15	8166.0
16	8166.0
17	8166.0
18	13494.0
19	18822.0
20	18822.0
21	18822.0
22	29667.0
23	40512.0
24	40512.0
25	40512.0
26	60255.0
27	79998.0
28	79998.0
29	79998.0
30	100280.0
31	120562.0
32	120562.0
33	120562.0
34	142833.0
35	165104.0
36	165104.0
37	165104.0
38	184270.0
39	203436.0
40	203436.0
41	203436.0
42	228095.0
43	252754.0
44	252754.0
45	252754.0
46	286172.5
47	319591.0
48	319591.0
49	319591.0
50	317190.0
51	314789.0
52	314789.0
53	314789.0
54	289651.5
55	264514.0
56	264514.0
57	264514.0
58	242835.5
59	221157.0
60	221157.0
61	221157.0
62	196685.5
63	172214.0
64	172214.0
65	172214.0
66	145084.0
67	117954.0
68	117954.0
69	117954.0
70	90989.0
71	64024.0
72	64024.0
73	64024.0
74	51132.0
75	38240.0
76	38240.0
77	38240.0
78	30563.0
79	22886.0
80	22886.0
81	22886.0
82	16580.5
83	10275.0
84	10275.0
85	10275.0
86	7554.5
87	4834.0
88	4834.0
89	4834.0
90	3292.0
91	1750.0
92	1750.0
93	1750.0
94	1085.5
95	421.0
96	421.0
97	421.0
98	702.0
99	983.0
100	983.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009111598780510127
2	0.003091435300530222
3	0.004962567192956409
4	0.01456228733670815
5	0.03591759697852876
6	0.04641220628822346
7	0.06691330354437125
8	0.09103463424456101
9	0.09738021196670199
10	0.11918296619149407
11	0.1212168052050008
12	0.13402999099009316
13	0.12264049251445551
14	0.1252438064517441
15	0.1150746113842105
16	0.11405769187745714
17	0.12414553338445047
18	0.13146735383307467
19	0.1326469804609086
20	0.12361673524093873
21	0.1309792324698331
22	0.1540022901027292
23	0.14074165973466535
24	0.13683668882873246
25	0.13321645538469049
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2458405.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.653315678820796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7230556298641	39.630002122930996
2	14.194992954183917	14.664369042421976
3	4.228435854856003	6.552381961155648
4	1.7276288585240174	3.569510352207276
5	0.8498004781399634	2.194750618068819
6	0.4849890661284779	1.5030776000106458
7	0.3242711770944155	1.1724777033200453
8	0.22137333419114297	0.914773337107856
9	0.1624780453059828	0.755327678856091
>10	0.8880045695929997	8.52261643631499
>50	0.09421049043641586	3.417780134876868
>100	0.08522332000448662	9.40457988493946
>500	0.011277561928901385	3.972199002253598
>1k	0.004179795679942193	3.4904154332237396
>5k	7.886406943287156E-5	0.23573869231205105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5787	0.23539652742326833	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3848	0.15652425047947754	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3739	0.15209048143003287	No Hit
GTACATGGAAGCAGTGGTATCAACG	3372	0.13716210307089355	No Hit
TCCATGTACTCTGCGTTGATACCAC	2853	0.11605085411069373	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2746	0.11169843862178933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.0676780270134496E-5	0.0
4	0.0	0.0	0.0	8.135356054026899E-5	0.0
5	0.0	0.0	0.0	8.135356054026899E-5	4.0676780270134496E-5
6	0.0	0.0	0.0	8.135356054026899E-5	4.0676780270134496E-5
7	0.0	0.0	0.0	8.135356054026899E-5	4.0676780270134496E-5
8	0.0	0.0	0.0	2.44060681620807E-4	4.0676780270134496E-5
9	0.0	0.0	0.0	3.6609102243121046E-4	4.0676780270134496E-5
10	0.0	0.0	0.0	3.6609102243121046E-4	4.0676780270134496E-5
11	0.0	0.0	0.0	4.88121363241614E-4	4.0676780270134496E-5
12	0.0	0.0	0.0	4.88121363241614E-4	1.220303408104035E-4
13	0.0	0.0	0.0	5.69474923781883E-4	1.220303408104035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	150	0.0	13.934617	16
CGAACGA	205	0.0	13.440264	16
CGTCTTA	115	5.329639E-9	12.391185	15
ACGGTAT	395	0.0	12.266954	9
ATAATAC	140	5.2750693E-11	12.210935	3
AACCGCG	180	0.0	12.140255	7
CGAACGT	110	3.8222424E-8	12.0898075	4
GTCTTAC	165	0.0	12.085131	1
CGTCGTA	335	0.0	11.911546	10
CGACCAT	305	0.0	11.525653	10
CGCCAGT	350	0.0	11.402678	18
ATCGCCA	375	0.0	11.401051	16
CTAATCT	125	1.835906E-8	11.398961	4
CGCTTAC	120	1.2721648E-7	11.085936	18
ACCGAGT	60	0.00587238	11.084581	8
CGCATCG	370	0.0	11.040658	13
ACGAACG	250	0.0	11.019894	15
TCGCCAG	380	0.0	11.001463	17
CCGTCGT	365	0.0	10.932514	9
AGGACGT	910	0.0	10.8570385	5
>>END_MODULE