FastQCFastQC Report
Thu 2 Feb 2017
SRR4062471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2458405
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA9750.019.860331
TAGGACG12550.018.5815624
CGCAATA6450.017.39524536
GTCCTAT6850.017.0252591
TTAGGAC10500.016.7618033
AGGACGT13850.016.5197565
TGTAGGA16950.016.3548812
GTATCAA64850.015.9476351
GCAATAC7100.015.80272237
TATACTG3500.015.7141915
CTGTAGG17050.015.6159871
GGACGTG13950.015.4551056
CAATACG6850.015.41596738
GTATTAT3150.015.3681131
TAGCGGC6900.015.30425630
TCTAGCG6900.015.30425628
CTAGCGG6900.015.30425629
TATTAGG2455.456968E-1215.2661462
TAGGACC16050.015.2148624
TATGGCG16100.015.1676116
Produced by FastQC (version 0.11.5)